Purification and Initial Characterization of Human Placental Trophoblast Interferon Induced by Polyriboinosinic {middle dot} Polyribocytidylic Acid

Aboagye-Mathiesen, George; Tóth, Ferenc; Juhl, Claus Bogh; Nørskov‐Lauritsen, Niels; Petersen, Peter M.; Ebbesen, Peter

doi:10.1099/0022-1317-71-12-3061

Cited by 18 publications

(3 citation statements)

References 25 publications

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“…Interferons (IFNs) or antiviral activity associated with IFNs are present in the pregnant mammalian uterus [6][7][8][9]. However, a role for IFNs during the period of early pregnancy has not been elucidated in species other than ruminants.…”

Section: Introductionmentioning

confidence: 98%

A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium1

Nagaoka

Sakai

Nojima

et al. 2003

Biology of Reproduction

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Proper distribution of immune cells in the uterus is a prerequisite for successful implantation and subsequent placentation, but biochemical signals that govern such events have not been well characterized. In the present study, the cDNA of a chemokine, interferon (IFN)-gamma-inducible protein 10 kDa (IP-10), was identified from a cDNA subtraction study between uterine endometrial tissues from Day 17 pregnant and Day 15 cyclic ewes. The effect of IFN-tau on IP-10 expression and the involvement of IP-10 in the recruitment of immune cells were then investigated. Northern blot analysis revealed that large amounts of IP-10 mRNA were present during conceptus attachment to maternal endometrium and early placentation. IP-10 mRNA was localized to monocytes distributed in the subepithelial stroma of pregnant but not cyclic uteri. This finding was supported by the discovery of IP-10 mRNA expression in monocytes but not in lymphocytes, uterine epithelial cells, or stromal cells. Moreover, the expression of IP-10 mRNA by the monocytes was stimulated by IFN-alpha, IFN-gamma, and IFN-tau in a dose-dependent manner, but the expression of IP-10 mRNA by the endometrial explants was most stimulated by IFN-tau. In a chemotaxis assay, migration of peripheral blood mononuclear cells was stimulated by the addition of IFN-tau stimulated-endometrial culture medium, and the effect was significantly reduced by neutralization with an anti-IP-10 antibody. These results suggest that endometrial IP-10 regulated by conceptus IFN-tau regulates recruitment and/or distribution of immune cells seen in the early pregnant uterus.

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Section: Introductionmentioning

confidence: 98%

A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium1

Nagaoka

Sakai

Nojima

et al. 2003

Biology of Reproduction

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“…However, no evidence for constitutive IFN production by first trimester cytotrophoblast cells (96) or placental tissue (97) was found by this laboratory. Recently, production of IFN-P has been described from full-term cytotrophoblast and choriocarcinoma cells that had been induced by virus or double-stranded RNA (98). Although touted as a "trophoblast IFN" and undoubtedly having potential importance in protecting the fetus against viral infection, it seems unlikely that this IFN has a role in the normal physiology of pregnancy, since noninduced tissue produced little or no activity.…”

Section: Ifn Expression In Trophoblast and Placenta Of Nonruminant Mamentioning

confidence: 98%

Role of Interferons in Maternal Recognition of Pregnancy in Ruminants

Roberts

Leaman

Cross

1992

Experimental Biology and Medicine

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It has recently become evident that a type I interferon (IFN) subtype signals the presence of a viable conceptus to the mother during early pregnancy in cattle, sheep, and related mammalian species. This IFN, which is a product of the epithelium (trophectoderm) of the expanding trophoblast, is expressed in extremely large quantities for a few days just prior to implantation. It appears to be involved in modulating the release of the luteolytic hormone, prostaglandin F2 alpha, from the uterine endometrium and, hence, preventing the destruction of the corpus luteum that normally occurs at the end of an estrous cycle if an egg has not been fertilized. These trophoblast IFN have antiviral, antiproliferative, and immunomodulatory properties quite similar to other type I IFN, such as IFN-alpha, -beta, and -omega. However, they constitute a structurally and serologically distinct subtype. In addition, they are poorly inducible by virus, and the promoter regions of their genes are organized differently than other type I IFN. The genes for these trophoblast IFN are confined to ruminant species in the Artiodactyla order and probably evolved from IFN-omega less than 55 million years ago. There is no evidence for comparable production of type I IFN by trophoblast and placental tissues of mammals outside this ruminant group. Recent experiments have indicated that IFN treatment may have value in improving reproductive performance of sheep when provided during the period of maternal recognition of pregnancy, when much embryonic loss is believed to occur.

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“…45 Finally, human trophoblast cultures have been found to produce another type of IFN, IFN-v, when infected by a virus. 46,47 Human IFN-t and -v mRNAs have been detected in citotrophoblast cells throughout pregnancy, suggesting that they protect the fetus from viral transmission and play a role in placental development. 45 In this study, we investigated IFN-g production by PBMCs stimulated with SEA, a bacterial superantigen, which activates large populations of T lymphocytes to proliferation and production of mainly T H 1-type cytokines.…”

Section: Aa In Plasmamentioning

confidence: 99%

Plasma Antiviral Activity and Interferon‐γ Production by Superantigen‐stimulated Lymphocytes during Normal Human Pregnancy

Grasso¹,

Massai²,

Migliaccio³

et al. 2001

American J Rep Immunol

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Purification and Initial Characterization of Human Placental Trophoblast Interferon Induced by Polyriboinosinic {middle dot} Polyribocytidylic Acid

Cited by 18 publications

References 25 publications

A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium1

A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium1

Role of Interferons in Maternal Recognition of Pregnancy in Ruminants

Plasma Antiviral Activity and Interferon‐γ Production by Superantigen‐stimulated Lymphocytes during Normal Human Pregnancy

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