Purification and immunochemical characterization of Streptococcus sanguis ATCC 10557 serotype II carbohydrate antigen

Kinoshita, Toshihiko; Okahashi, Nobuo; Yamamoto, Toshiko; Mizuno, Junn; Inoue, Makoto; Hamada, Shigeyuki

doi:10.1128/iai.42.2.696-700.1983

Cited by 12 publications

(7 citation statements)

References 23 publications

(30 reference statements)

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“…In addition to epitopes that do not include the Gn or G motifs, other epitopes may overlap with these regions as suggested by the demonstration of cross-reactivity between the 2G and 3G polysaccharides. A possible structural basis for these cross-reactions is suggested by the previous identification of GalNAc as the immunodominant sugar of the 3G polysaccharide, a molecule designated as the serotype II carbohydrate in earlier studies (22,38). Significantly, the only GalNAc unit in the 3G structure is GalNAc␣ in the G motif ( Table 2).…”

Section: Discussionmentioning

confidence: 92%

Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins

et al. 1997

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Lectin-mediated interactions between oral viridans group streptococci and actinomyces may play an important role in microbial colonization of the tooth surface. The presence of two host-like motifs, either GalNAc␤133Gal (Gn) or Gal␤133GalNAc (G), in the cell wall polysaccharides of five streptococcal strains accounts for the lactose-sensitive coaggregations of these bacteria with Actinomyces naeslundii. Three streptococcal strains which have Gn-containing polysaccharides also participate in GalNAc-sensitive coaggregations with strains of Streptococcus gordonii and S. sanguis. Each Gn-or G-containing polysaccharide is composed of a distinct phosphodiester-linked hexa-or heptasaccharide repeating unit. The occurrence of these polysaccharides on 19 additional viridans group streptococcal strains that participate in lactose-sensitive coaggregations with actinomyces was examined. Negatively charged polysaccharides that reacted with Bauhinia purpurea agglutinin, a Gal and GalNAc binding plant lectin, were isolated from 17 strains by anion exchange column chromatography of mutanolysin-cell wall digests. Results from nuclear magnetic resonance and immunodiffusion identified each of 16 polysaccharides as a known Gn-or G-containing structural type and one polysaccharide as a new but closely related Gn-containing type. Unlike the reactions of lectins, the cross-reactions of most rabbit antisera with these polysaccharides were correlated with structural features other than the host-like motifs. Gn-containing polysaccharides occurred primarily on the strains of S. sanguis and S. oralis while G-containing polysaccharides were more common among the strains of S. gordonii and S. mitis examined. The findings strongly support the hypothesis that lectin-mediated recognition of these streptococci by other oral bacteria depends on a family of antigenically diverse Gn-and G-containing cell wall polysaccharides, the occurrence of which may differ between streptococcal species.

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Section: Discussionmentioning

confidence: 92%

Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins

et al. 1997

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“…Results from previous studies of the 10557 polysaccharide suggested that GalNAc was an immunodeterminant (Koga et al, 1983); however, this proposal was based on the inhibition of immunoprecipitation by a very high concentration of the monosaccharide. Further studies with oligosaccharide fragments of these polysaccharides are in progress to firmly establish their respective antigenic and receptor regions.…”

Section: Discussionmentioning

confidence: 99%

“…When applied to a calibrated column of Sephacryl S-400 (Pharmacia) in a 0.1 M NaCl, 0.01 M Tris HC1 buffer (pH 7.6), the 10557 polysaccharide emerged at a position similar to that of a clinical dextran (Sigma D4751) with average molecular mass of 80 kDa determined by light scattering. The 10557 polysaccharide gave a reaction of complete identity with a sample of the previously studied ATCC 10557 serotype II carbohydrate antigen (Koga et al, 1983) kindly provided by Dr. S. Hamada (Osaka University, Suita-Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Streptococcus oralis ATCC 10557 was isolated from a patient with subacute bacterial endocarditis, and, like strains 34 and J22, this organism participates in lectin-mediated coaggregations with representative Actinomyces strains (Sato et al, 1984). The cell wall polysaccharide of strain 10557 was previously shown to contain GalNAc, Gal, Rha, and Glc along with some phosphate (Koga et al, 1983) and also was found to inhibit the lactose-sensitive coaggregations of strain 10557 with A. viscosus 19246 (Sato et al, 1984) and of S. oralis 34 with A. viscosus T14V (Mclntire et al, 1988). In spite of their similar inhibitory activities, antiserum against strain 34 failed to react with the 10557 polysaccharide.…”

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confidence: 99%

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Complete structure of the cell surface polysaccharide of Streptococcus oralis ATCC 10557: a receptor for lectin-mediated interbacterial adherence

Abeygunawardana¹,

Bush²,

Cisar³

1991

Biochemistry

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Lectin-carbohydrate binding is known to play an important role in a number of different cell-cell interactions including those between certain species of oral streptococci and actinomyces that colonize teeth. The cell wall polysaccharides of Streptococcus oralis ATCC 10557, S. oralis 34, and Streptococcus mitis J22, although not identical antigenically, each function as a receptor molecule for the galactose and N-acetylgalactosamine reactive fimbrial lectins of Actinomyces viscosus and Actinomyces naeslundii. Carbohydrate analysis of the receptor polysaccharide isolated from S. oralis ATCC 10557 shows galactose (3 mol), glucose (1 mol), GalNAc (1 mol), and rhamnose (1 mol). 1H NMR spectra of the polysaccharide show that is is partially O-acetylated. Analysis of the 1H NMR spectrum of the de-O-acetylated polysaccharide shows that it is composed of repeating subunits containing six monosaccharides and that the subunits are joined by a phosphodiester linkage. The 1H and 13C NMR spectra were completely assigned by two-dimensional homonuclear correlation methods and by 1H-detected heteronuclear multiple-quantum correlation (1H[13C]HMQC). The linkage of the component monosaccharides in the polymer, deduced from two-dimensional 1H-detected heteronuclear multiple-bond correlation spectra (1H[13C]HMBC), shows that the repeating unit of the de-O-acetylated polymer is a linear hexasaccharide with no branch points. The complete 1H and 13C assignment of the native polysaccharide was carried out by the same techniques augmented by a 13C-coupled hybrid HMQC-COSY method, which is shown to be especially useful for carbohydrates in which strong coupling and overlapping peaks in the 1H spectrum pose difficulties. The fully assigned spectra of the native polymer show that each of two different positions is acetylated in one-third of the repeating subunits and that the acetylation is randomly distributed along the polymer. The exact positions of acetylation were assigned by a carbonyl-selective HMBC method that unambiguously defines the positions of O-acetylation. The complete structure of the native polysaccharide in S. oralis ATCC 10557 is [formula: see text] Comparison of this structure with those previously determined for the polysaccharides of strains 34 and J22 suggests that the similar lectin receptor activities of these molecules may depend on internal galactofuranose linked (beta 1----6)- to Gal(beta 1----3)GalNAc(alpha) or GalNAc(beta 1----3)Gal(alpha).

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“…Immunodiffusion was performed in 1% (wt/vol) Noble agar (Difco) in saline (13). Quantitative precipitin reaction and hapten inhibition tests were performed as previously described (13). Monosaccharides and the partial hydrolysate of the purified antigen were used as haptens.…”

Section: Methodsmentioning

confidence: 99%

Immunochemical and structural characterization of a serotype-specific polysaccharide antigen from Actinobacillus actinomycetemcomitans Y4 (serotype b)

et al. 1989

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A serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b) was extracted from whole cells by autoclaving. The extract was purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300 columns. The purified polysaccharide antigen formed a single precipitin line with anti-type b serum but not with anti-type a serum and anti-type c serum. The antigen was composed of 43.9% L-rhamnose, 49.1% D-fucose, and a trace amount of fatty acid. Methylation analysis, Smith degradation, and optical rotation data showed that the antigen was a polymer consisting of a disaccharide repeating unit,-3)-aD -fucopyranosyl-(1-*2)-,1-L-rhamnopyranosyl-(l-. In quantitative precipitin inhibition tests, D-fucose and L-rhamnose showed very low inhibition, but the partial hydrolysate of the purified antigen was an effective inhibitor, suggesting that the serotype b specific antiserum recognizes the larger oligosaccharide units.

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Purification and immunochemical characterization of Streptococcus sanguis ATCC 10557 serotype II carbohydrate antigen

Cited by 12 publications

References 23 publications

Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins

Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins

Complete structure of the cell surface polysaccharide of Streptococcus oralis ATCC 10557: a receptor for lectin-mediated interbacterial adherence

Immunochemical and structural characterization of a serotype-specific polysaccharide antigen from Actinobacillus actinomycetemcomitans Y4 (serotype b)

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