Purification and identification of novel antioxidant peptides from watermelon seed protein hydrolysates and their cytoprotective effects on H2O2-induced oxidative stress

Wen, Chaoting; Zhang, Jixian; Feng, Yiyu; Duan, Yuqing; Ma, Haile; Zhang, Haihui

doi:10.1016/j.foodchem.2020.127059

Cited by 106 publications

(94 citation statements)

References 32 publications

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“…The performance of enzymatic hydrolysis can be improved by the previous treatment of proteins with ultrasound. Indeed, Wen et al demonstrated that ultrasound treatment had a significant impact on proteins structure by improving their susceptibility to hydrolysis [51,53]. They submitted watermelon seed proteins to three different ultrasound treatments-single (20 kHz), dual (20 kHz/28 kHz), and tri-frequency (20/28/40 kHz))-before hydrolysis with Alcalase.…”

Section: Methods Used For the Release Of Antioxidant Peptidesmentioning

confidence: 99%

“…Molecular weights between 0.5 and 1.5 kDa are also a common feature within antioxidant peptides [22]. Antioxidant peptides have been obtained from rapeseed residues (Brassica napus) [23,24], peels of pomegranate (Punica granatum) [3,25] and mango (Mangifera indica) [26], and seeds of apricot (Prunus armeniaca) [11,27], peach (Prunus persica L.) [11,16,28,29], bottle gourd (Lagenaria sciceraria) [30][31][32], cherry (Prunus cerasus L.) [11,33], olive (Olea europaea) [11,16,17,34], plum (Prunus domestica L.) [11,14,29,35], tomato (Solanum lycopersicum) [21,[36][37][38][39][40][41][42][43], wax gourd (Benincasa hispida) [44], jujube (Ziziphus jujube) [45,46], muskmelon (Cucumis melo) [47], watermelon (Citrullus lanatus) [32,[48][49][50][51][52][53], papaya (Carica papaya)…”

Section: Antioxidant Peptides In Fruit Residuesmentioning

confidence: 99%

“…The method evaluates the reduction in the absorbance of ABTS radicals due to the presence of potential antioxidants. [3,11,14,16,17,20,24,25,28,31,[33][34][35][36][37]41,42,45,46,49,51,52,54,55,58,62,65] Scavenging effect on nitric oxide (NO) radicals The assay measures the rate of pyrogallol autooxidation in presence and absence of potential antioxidants at 320-420 nm. [50,55] Inhibition of formation of hydroxyl (OH•) radicals…”

Section: Evaluation Of Antioxidant Activity Of Peptidesmentioning

confidence: 99%

“…The inhibition of fluorescein oxidation by the presence of potential antioxidants is measured from the increase in fluorescence intensity. [51,65] EVALUATION OF THE REDUCING POWER…”

Section: Evaluation Of Antioxidant Activity Of Peptidesmentioning

confidence: 99%

“…The presence of a potential antioxidant inhibits ROS generation and DCF signal decreases. [16,51] Intracellular concentration of Ca 2+ determination Intracellular Ca 2+ is measured with fluorescent dye Fura-2 AM. Fura-2AM is cleaved by intracellular esterase, and the resulting Fura-2 can bind to Ca 2+ and cause strong fluorescence under a 330-350 nm excitation light.…”

Section: Evaluation Of the Capacity To Inhibit Dna Oxidationmentioning

confidence: 99%

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Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Olivares-Galván

Marina

García

2020

Foods

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Fruit residues with high protein contents are generated during the processing of some fruits. These sustainable sources of proteins are usually discarded and, in all cases, underused. In addition to proteins, these residues can also be sources of peptides with protective effects against oxidative damage. The revalorization of these residues, as sources of antioxidant peptides, requires the development of suitable methodologies for their extraction and the application of analytical techniques for their characterization. The exploitation of these residues involves two main steps: the extraction and purification of proteins and their hydrolysis to release peptides. The extraction of proteins is mainly carried out under alkaline conditions and, in some cases, denaturing reagents are also employed to improve protein solubilization. Alternatively, more sustainable strategies based on the use of high-intensity focused ultrasounds, microwaves, pressurized liquids, electric fields, or discharges, as well as deep eutectic solvents, are being implemented for the extraction of proteins. The scarce selectivity of these extraction methods usually makes the subsequent purification of proteins necessary. The purification of proteins based on their precipitation or the use of ultrafiltration has been the usual procedure, but new strategies based on nanomaterials are also being explored. The release of potential antioxidant peptides from proteins is the next step. Microbial fermentation and, especially, digestion with enzymes such as Alcalase, thermolysin, or flavourzyme have been the most common. Released peptides are next characterized by the evaluation of their antioxidant properties and the application of proteomic tools to identify their sequences.

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Section: Methods Used For the Release Of Antioxidant Peptidesmentioning

confidence: 99%

Section: Antioxidant Peptides In Fruit Residuesmentioning

confidence: 99%

Section: Evaluation Of Antioxidant Activity Of Peptidesmentioning

confidence: 99%

“…The inhibition of fluorescein oxidation by the presence of potential antioxidants is measured from the increase in fluorescence intensity. [51,65] EVALUATION OF THE REDUCING POWER…”

Section: Evaluation Of Antioxidant Activity Of Peptidesmentioning

confidence: 99%

Section: Evaluation Of the Capacity To Inhibit Dna Oxidationmentioning

confidence: 99%

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Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Olivares-Galván

Marina

García

2020

Foods

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Chinese Propolis: Ultrasound‐assisted enhanced ethanolic extraction, volatile components analysis, antioxidant and antibacterial activity comparison

Ding

Sheikh

et al. 2020

Food Science & Nutrition

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This study was aimed to enhance the extraction yield of propolis samples using ultrasound technology, analyze the volatile compounds, and compare the antioxidant and antimicrobial effect of propolis extracts of different areas. Four propolis samples were collected from different regions of China, namely: Linqing, Shandong Province (LSP); Yingchun, Heilongjiang Province (YHP); Changge, Henan Province (CHP); and Raohe, Heilongjiang Province (RHP). The ultrasound extracts of CHP and RHP showed a higher total phenolic content (TPC) of 201.78 ± 4.60 mgGAE/g and 166.071 ± 1.53 mgGAE/g, total flavonoid content (TFC) of 519.77 ± 29.90 and 341.227 ± 10.82 mg quercetin/g respectively, as well as high antioxidant and antibacterial activity. Conventional extraction showed 15%–20% lower yield for TPC ranging from 72.02 ± 1.99 to 155.95 ± 3.69 mg GAE/g, TFC ranges from 129.675 ± 6.82 to 412.83 ± 12.14 mg quercetin/g, with lower antibacterial activity. The antioxidant activity of propolis extracts was determined by assays of reducing power, DPPH*, FRAP*, TEAC*, hydroxyl radical scavenging activity and superoxide anion scavenging activity. Collectively, the antioxidant activities of extracts from CHP and RHP were higher than those of the other two extracts(YHP and LSP). All the extracts showed high antimicrobial activity on Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis, but no effect on Escherichia coli. A total of 150 compounds in propolis were detected by GC/MS. Terpenes (RHP 34%, YHP 5%, LSP 18%, and CHP 12%) and alcohols (RHP 12%, YHP 13%, LSP 12%, and CHP 10%) showed the highest relative content among all other extracts.

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Identification of novel antioxidant collagen peptides for preventing and treating H₂O₂‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches

Du,

Zhang,

Deng

et al. 2023

J Sci Food Agric

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BACKGROUNDNowadays, the prevalence of oxidative stress‐related chronic diseases is increasing. The identification of novel antioxidant collagen peptides to counteract oxidative stress for individuals' health has gained significant attention.RESULTSIn this study, collagen peptides with antioxidant activities were separated and identified by ion chromatography, reversed‐phase high‐performance liquid chromatography and liquid chromatography–tandem mass spectrometry. The identified antioxidant collagen peptides were further screened by molecular docking for Keap1‐targeted peptide inhibitors and their theoretical interaction mechanisms were investigated. Four novel antioxidant collagen peptides, GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA, with high binding affinity to Keap1 were selected. Molecular docking results demonstrated that the putative antioxidant mechanism of the four antioxidant collagen peptides contributed to their blockage of Keap1–Nrf2 interactions. The results of antioxidant activity of the four antioxidant collagen peptides proved that IDGRPGPIGPA exerted a high scavenging capacity for DPPH and ABTS free radicals, while GPAGPpGPIG improved the resistance of cells to hydrogen peroxide‐induced oxidative damage by promoting the activation of intracellular antioxidant enzymes and the production of reduced glutathione in human hepatoma (HepG2) cells.CONCLUSIONThe antioxidant collagen peptides (GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA) will be developed as novel functional food for human health in the near future. © 2023 Society of Chemical Industry.

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Purification and identification of novel antioxidant peptides from watermelon seed protein hydrolysates and their cytoprotective effects on H2O2-induced oxidative stress

Cited by 106 publications

References 32 publications

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Chinese Propolis: Ultrasound‐assisted enhanced ethanolic extraction, volatile components analysis, antioxidant and antibacterial activity comparison

Identification of novel antioxidant collagen peptides for preventing and treating H₂O₂‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches

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Purification and identification of novel antioxidant peptides from watermelon seed protein hydrolysates and their cytoprotective effects on H2O2-induced oxidative stress

Cited by 106 publications

References 32 publications

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Chinese Propolis: Ultrasound‐assisted enhanced ethanolic extraction, volatile components analysis, antioxidant and antibacterial activity comparison

Identification of novel antioxidant collagen peptides for preventing and treating H2O2‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches

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Product

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Identification of novel antioxidant collagen peptides for preventing and treating H₂O₂‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches