Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates

Separation and purification of angiotensin-I-converting enzyme (ACE) inhibitory peptides from walnuts (Juglans regia L.) meal

“…Studies of walnut protein or peptides were reported previously, e.g., the chemical composition, molecular weight distribution of walnut protein isolates and concentrates [17], the effect of different oil extraction methods on protein characteristics of walnut [18], and the antioxidant activities of the walnut hydrolysates [19]. Statistics show that China's walnut production is ranked first globally, but it is still far from self-sufficient in terms of processing capabilities and utilization.…”

Purification and identification of angiotensin I-converting enzyme inhibitory peptides from fermented walnut residues

“…The contents of the filtrated protein and free amino acid were determined by the Kjeldahl method and an automatic amino acid analyser (Hitachi L-8900, Tokyo, Japan), respectively. [17] The peptide content and the yield of peptide were then calculated according to Eqs. (1) ACE-inhibition pattern determination of the purified peptides…”

“…The inhibition pattern was determined by spectrophotometry. [16] ACE kinetics in the presence of the inhibitor were analysed by Lineweaver-Burk plots [17] at three peptide concentrations (0, 0.2, and 0.4 mg/mL), in which the reciprocal of HHL concentration was used as an independent variable (X-axis), and the reciprocal of the HA production rate was used as a dependent variable (Y-axis). The Lineweaver-Burk plot, showing correlations between 1/V and 1/[S], was used to determine the X-axis intercepts of −1/K m (without inhibitor) and −1/{K m (1 + [I]/K i )} (with inhibitor), which were used to calculate the inhibitory constant K i .…”

Antimicrobial, antioxidant and anti-inflammatory assessment of a phytocosmetic produced with glycinin peptides

“…36,37,38,4 Alanine, aspartic acid, phenylalanine, histidine, tyrosine, methionine, cysteine are responsible antioxidant capacity peptides. 39,40 And the glycinin constituted in aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine and arginine amino acids. 14 However, the amino acids arginine, lysine, histidine (cationic), alanine, leucine, isoleucine and methionine (hydrophobic) are responsible for the connections with the epithelial cells of the stratum corneum, which have negative charges, increasing their permeability and easing the passage of the phytocosmetic.…”