Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides from Royal Jelly Treated with Protease

Maruyama, Haruhiko; Tokunaga, Katsuhiko; Suzuki, Kunihiro; Yoshida, Chie; Futamura, Yoshihiro; Araki, Yasufumi; Mishima, Satoshi

doi:10.3136/nskkk.50.310

Cited by 13 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5) They have an antihypertensive effect in repeated oral administration for 28 d on SHR. 6) In this study, we investigated the contributive ratio of these peptides in ProRJ for antihypertensive effect and MIX in single oral administration using SHR.…”

Section: Discussionmentioning

confidence: 99%

“…We have shown that Protease N treated RJ (ProRJ) and peptides (IY, VY, IVY) from ProRJ inhibited angiotensin I-converting enzyme (ACE) activity 6) and they have an antihypertensive effect in repeated oral administration for 28 d on spontaneously hypertensive rats (SHR). 7) Their ACE inhibitory activity (IC 50 ) of IY, VY and IVY are 0.008, 0.020 and 0.018 (mg/ml) respectively.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Tokunaga¹,

Yoshida²,

Suzuki³

et al. 2004

Biological & Pharmaceutical Bulletin

Self Cite

135

View full text Add to dashboard Cite

We have shown that Protease N treated Royal Jelly (ProRJ) and peptides from ProRJ (Ile-Tyr (IY), Val-Tyr (VY), Ile-Val-Tyr (IVY)) inhibited angiotensin I-converting enzyme (ACE) activity and they have an antihypertensive effect in repeated oral administration for 28 d on spontaneously hypertensive rats (SHR). We investigated the contributive ratio of these peptides in ProRJ for antihypertensive effect in single oral administration on SHR. In single oral administration of each peptide and peptides mixture (MIX; IY, VY and IVY) at doses of 0.5, 1 and 10 mg/kg, systolic blood pressure (SBP) of SHR was reduced dose-dependently. This antihypertensive effect was held for 8 h. These results suggest that peptides contributed to the antihypertensive effect of ProRJ. And the contributive ratio of MIX in ProRJ for antihypertensive effect was computed to be about 38%. Therefore it is considered that intake of peptides, as a functional food would be beneficial for improving blood pressure in people with hypertension.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Tokunaga¹,

Yoshida²,

Suzuki³

et al. 2004

Biological & Pharmaceutical Bulletin

Self Cite

135

View full text Add to dashboard Cite

show abstract

“…3.4.15.1) inhibitory activity, which contributes to the regulation of blood pressure [5][6][7][8]. Among them, Tyr-Tyr is isolated from royal jelly treated with protease [8]. According to some research [9,10], TyrTyr is effectively transported through PEPT1, a proton-coupled peptide transporter expressed on the intestinal epithelium.…”

Section: Introductionmentioning

confidence: 98%

“…Furthermore, a number of Tyr-containing di-or tri-peptides from several food protein sources exhibit angiotensin I-converting enzyme (ACE, EC. 3.4.15.1) inhibitory activity, which contributes to the regulation of blood pressure [5][6][7][8]. Among them, Tyr-Tyr is isolated from royal jelly treated with protease [8].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides

Mitsuhashi

Nakayama

Narai-Kanayama

2015

Enzyme and Microbial Technology

View full text Add to dashboard Cite

“…Some of the peptides from RJ have recently shown antihypertensive effects in spontaneously hypertensive rats (SHR) by inhibiting ACE (9)(10)(11)(12). The inhibitors of ACE have been studied previously (13,14), but their potential interaction with the kallikrein system (15,16) and their inhibition of other enzymes unrelated to the RA system cloud interpretation of their physiologic responses (17).…”

Section: Introductionmentioning

confidence: 99%

A dipeptide YY derived from royal jelly proteins inhibits renin activity

Sultana

Nabi²,

Nasir³

et al. 2008

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Abstract. Renin is the rate limiting enzyme in the reninangiotensin (RA) system that regulates blood pressure and electrolyte balance. In this study, we investigated the renin inhibitory effect of a royal jelly (RJ)-derived peptide. A dipeptide YY was isolated from the digested fraction of RJ proteins by proteases and was found to inhibit human renin activity. The inhibition constant (K i ) of YY was estimated to be 10 μM when the K m was 0.16 μM using sheep angiotensinogen as the substrate. The peptide was observed to lower blood pressure in spontaneously hypertensive rats.

show abstract

Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides from Royal Jelly Treated with Protease

Cited by 13 publications

References 0 publications

Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

Mechanism of papain-catalyzed synthesis of oligo-tyrosine peptides

A dipeptide YY derived from royal jelly proteins inhibits renin activity

Contact Info

Product

Resources

About