Purification and Identification of a Growth-Stimulating Peptide for<i>Bifidobacterium bifidum</i>from Natural Rubber Serum Powder

Etoh, S.; Asamura, Kayoko; Obu, Azumi; Sonomoto, Kenji; Ishizaki, Ayaaki

doi:10.1271/bbb.64.2083

Cited by 22 publications

(16 citation statements)

References 19 publications

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“…3, the growth‐stimulating activity of CHPu or CHTu was higher than other ultrafiltered fractions. These findings were similar to the previous finding that peptides with highly hydrophilic residua were more active in stimulating the growth of Bifidobacterium (Etoh et al. , 2000).…”

Section: Resultssupporting

confidence: 92%

Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Zhang

Ren

Zhao

et al. 2011

Int J of Food Sci Tech

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The growth performance of Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus) and Streptococcus thermophilus (S. thermophilus) was determined in the presence of casein hydrolysates produced by the action of five proteolytic enzymes (Alcalase, Flavorzyme, Neutrase, Papain and Trypsin) with various degrees of hydrolysis (DH). In addition, these five kinds of casein hydrolysates were fractionated by ultrafiltration and the influence of the amino acid composition of the peptides on the growth and lactic acid yield of yoghurt lactic acid bacteria (LAB) was studied. The results showed that the ultrafiltered fraction (<3000 Da) was the determinant stimulator in crude hydrolysates. Furthermore, the hydrophilic amino acid residua including His, Lys, Glu and Ser were beneficial for bacterial growth. Compared with control, the cell growth and lactic acid yield of yoghurt LAB were increased with the supplementation of the peptides fraction (<3000 Da) produced with papain by 65.1% and 49.6%, respectively.

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Section: Resultssupporting

confidence: 92%

Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Zhang

Ren

Zhao

et al. 2011

Int J of Food Sci Tech

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“…This adds to the ®ndings of Ibrahim and Bezkorovainy who suggest that the diverse bi®dobacterial growth-promoting factors reported have a common component, namely sulfur containing peptides. In contrast with these ®ndings Etoh et al [20] identi®ed a decapeptide with growth promoting activity towards B. bi®dum, which contains neither cystine nor cysteine. Therefore, they suggest that sulfhydryl groups are not indispensable to growth-stimulating activity.…”

Section: Discussionmentioning

confidence: 89%

“…In␣contrast with these findings Etoh et al . [20] identified a decapeptide with growth promoting activity towards B. bifidum , which contains neither cystine nor cysteine. Therefore, they suggest that sulfhydryl groups are not indispensable to growth‐stimulating activity.…”

Section: Discussionmentioning

confidence: 99%

Human milk provides peptides highly stimulating the growth of bifidobacteria

Liepke¹,

Adermann²,

Raida³

et al. 2002

European Journal of Biochemistry

188

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The large intestine of breast‐fed infants is colonized predominantly by bifidobacteria, which have a protective effect against acute diarrhea. In this study we report for the first time the identification of human milk peptides that selectively stimulate the growth of bifidobacteria. Several bifidogenic peptides were purified chromatographically from pepsin‐treated human milk and identified as proteolytically generated fragments from the secretory component of the soluble polyimmunoglobulin receptor and lactoferrin; both of these proteins exhibit antimicrobial effects. Hydrolysis of the identified peptides with the gastrointestinal proteases pepsin, trypsin and chymotrypsin did not lead to the loss of bifidogenic activity, indicating their potential function in vivo. Sequential comparison revealed a similar structural motif within the identified peptides. A correspondingly designed small peptide (prebiotic lactoferrin‐derived peptide‐I, PRELP‐I) was found to stimulate the growth of bifidobacteria as effectively as the native peptides. The combination of antimicrobial and bifidobacterial growth stimulatory activity in human milk proteins leads to highly specific compounds capable of regulating the microbial composition of infants' large intestine.

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“…() reported that bovine GMP that contained sialic acid stimulated the growth of B. infantis . Peptides with hydrophilic residues were found to be more effective in promoting the growth of bifidobacteria (Etoh et al ., ; Zhang et al ., ). On the contrary, Poch & Bezkorovainy () observed that bifidobacterium growth promoter existed in para‐κ‐casein rather than GMP.…”

Section: Introductionmentioning

confidence: 99%

Bifidobacterial growth–promoting effect of yak milk κ‐casein hydrolysates produced with different proteases

Tang

Tian

Cheng

et al. 2013

Int J of Food Sci Tech

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Summary The growth‐promoting effects of κ‐casein hydrolysates produced by four different proteases (trypsin, alcalase, papain and chymosin) on bifidobacteria (Bb12 and BBMN68) viability were evaluated. Results showed that the obtained κ‐casein hydrolysates possessed better property for improving the viability of Bb12 and BBMN68 than native κ‐casein. The bifidobacterium viability and pH drop were dependent on the added hydrolysates. The addition of κ‐casein hydrolysates improved the viability of Bb12 and BBMN68 to a variable extent. The κ‐casein hydrolysate produced with papain (KCHP) is the best for Bb12 and κ‐casein hydrolysate produced with trypsin (KCHT) is the best for BBMN68. Proper amino acid profiles improved the viability of Bb12 and BBMN68. The bifidobacterial growth‐promoting capacity of κ‐casein hydrolysate may be due to its high content of Ala, Met, Tyr, Phe and Arg residues, especially Tyr which is richer in para‐κ‐casein than in casein glycomacropeptide (GMP). In addition, the sialic acid content of κ‐casein hydrolysate produced with the tested proteases did not show a direct relationship with its bifidobacterial growth–promoting effect. The obtained peptides possessed growth‐promoting activity for Bb12 and BBMN68 existed in para‐κ‐casein.

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Purification and Identification of a Growth-Stimulating Peptide forBifidobacterium bifidumfrom Natural Rubber Serum Powder

Cited by 22 publications

References 19 publications

Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Influence of casein hydrolysates on the growth and lactic acid production of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Human milk provides peptides highly stimulating the growth of bifidobacteria

Bifidobacterial growth–promoting effect of yak milk κ‐casein hydrolysates produced with different proteases

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