Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon

Abstract

ABSTRACT. The causative viral agent was purified from diseased shrimp Penaeus monodon with white spot syndrome. Negatively stained preparations show that the virus is pleiomorphic. It is fusiform or rod-shaped. In negatively stained preparations, the virion measures 70 to 150 nm at its broadest point and is 250 to 380 nm long. In some virions, a tail-like projection extends from one end. The capsid is apparently composed of rings of subunits in a stacked series. The rings are aligned perpendicular to the longitudinal axis of the capsid. The genome of the virus is a double-stranded DNA molecule which produces at least 22 Hind 111 fragments. The full length of the DNA is estimated to be longer than 150 kbp. Based on the morphological characteristics and genomic structures of the virus, we confirm that white spot syndrome associated virus (MJSSV) is a member of genus NOB (Non-Occluded Baculovirus) of the subfamily Nudibaculovirinae of Baculoviridae, name the present isolate PmNOBIII, and propose the use of WSBV (Baculovirus associated with White Spot syndrome) to indicate PmNOBIII related agents.

Association of dual viral infection with mortality of Pacific white shrimp (Litopenaeus vannamei) in culture ponds in India

“…WSSV is a double-stranded rod-shaped DNA virus and belongs to the family Nimaviridae [17]. This virus is well known for its high virulence and thereby bringing severe mortality within a short span of time [19,34]. IHHNV was discovered as early as 1981 when it was highly virulent to Penaeus stylirostris resulting in high mortality [14].…”

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

“…1995). WSSV is an enveloped virus with a large, double-stranded, circular DNA genome (∼300 kb) containing approximately 180 putative open reading frames (ORFs), most of which have no homology with any known genes or proteins in public databases Wongteerasupaya et al 1995;Wang et al 1995;Chang et al 1996;Chen et al 1997;Yang et al 2001). Proper husbandry and management of farms with the application of immunostimulants, probiotics and bioremediators can save the industry from the onslaught of diseases to a certain extent.…”

Generation of recombinant monoclonal antibodies to study structure–function of envelope protein VP28 of white spot syndrome virus from shrimp

“…It is an enveloped, non-occluded, rod-shaped and circular double-stranded DNA (about 300 kbp) virus [3][4][5][6]. Based on its unique genetic information and morphological feature, WSSV was classified in a new virus family, Nimaviridae in 2002 [7].…”