Purification and cloning of lectin that induce cell apoptosis from Allium chinense

Xiao, Xiuqing; He, Hao; Ding, Xuezhi; Yang, Qi; Liu, Xuemei; Liu, Shuang; Rang, Jie; Wang, Ting; Zuo, Mingxing; Xia, Liqiu

doi:10.1016/j.phymed.2014.12.004

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…Plant lectins represent the most studied group of carbohydrate‐binding proteins, which comprises a large group of homologous proteins found primarily in the seeds. However, lectins isolated from other plant components, such as bulb and rhizome, have also been reported (Loris et al ., ; Prasanna and Venkatesh, ; Xiao et al ., ; Albuquerque et al ., ). Leguminous seeds are a rich source of lectins, and several genera of the subtribe Diocleinae (tribe Phaseoleae ) have been the main source for isolation and characterization of novel lectins (Cavada et al ., ; Peumans and Van Damme, Correia et al ., ; Van Damme et al ., ; Rocha et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

Pinto-Junior

Correia

Pereira

et al. 2015

J of Molecular Recognition

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A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α-methyl-D-mannoside, D-mannose, and D-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25,562, 12,874, and 12,706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp.

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Section: Introductionmentioning

confidence: 99%

Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

Pinto-Junior

Correia

Pereira

et al. 2015

J of Molecular Recognition

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“…The endogenous lectin, galectin1 promoted anoikis in cancer cells by inhibiting the signaling through α5β1 integrin, the FN receptor present on the cell surface ( Sanchez-Ruderisch et al, 2011 ). The anticancer activity of lectins from various sources was studied previously ( Panda et al, 2014 ; Xiao et al, 2015 ; Singh et al, 2016 ). But anoikis inducing effects of lectin purified from any natural sources are seldom reported.…”

Section: Discussionmentioning

confidence: 99%

Morus alba Leaf Lectin (MLL) Sensitizes MCF-7 Cells to Anoikis by Inhibiting Fibronectin Mediated Integrin-FAK Signaling through Ras and Activation of P38 MAPK

et al. 2017

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Lectins are a unique class of carbohydrate binding proteins/glycoproteins, and many of them possess anticancer properties. They can induce cell cycle arrest and apoptosis, inhibit protein synthesis, telomerase activity and angiogenesis in cancer cells. In the present study, we have demonstrated the effect of Morus alba leaf lectin (MLL) on anoikis induction in MCF-7 cells. Anoikis induction in cancer cells has a significant role in preventing early stage metastasis. MLL treatment in monolayers of MCF-7 cells caused significant detachment of cells in a time and concentration dependent manner. The detached cells failed to re-adhere and grew even to culture plates coated with different matrix proteins. DNA fragmentation, membrane integrity studies, annexin V staining, caspase 9 activation and upregulation of Bax/Bad confirmed that the detached cells underwent apoptosis. Upregulation of matrix metalloproteinase 9 (MMP-9) caused a decrease in fibronectin (FN) production which facilitated the cells to detach by blocking the FN mediated downstream signaling. On treatment with MLL, we have observed downregulation of integrin expression, decreased phosphorylation of focal adhesion kinase (FAK), loss in FAK-integrin interaction and active Ras. MLL treatment downregulated the levels of phosphorylated Akt and PI3K. Also, we have studied the effect of MLL on two stress activated protein kinases p38 MAPK and JNK. p38 MAPK activation was found to be elevated, but there was no change in the level of JNK. Thus our study substantiated the possible antimetastatic effect of MLL by inducing anoikis in MCF-7 cells by activation of caspase 9 and proapoptotic Bax/Bad by blockage of FN mediated integrin/FAK signaling and partly by activation of p38 MAPK.

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“…Allium chinense lectins (ACL) were studied on Hep-3B human hepatoma cells, where a cytotoxic effect was observed in a dose-dependent manner. Apoptosis by mitochondrial route was determined [ 54 ]. Additionally, Broccolini lectin (BL) from Brassica oleracea Italica showed a selective dose-dependent cytotoxic effect on HepG2 cells [ 162 ].…”

Section: Plant Lectins Against Liver Cancermentioning

confidence: 99%

Plant Lectins as Medical Tools against Digestive System Cancers

Estrada-Martínez

Moreno-Celis

Cervantes-Jiménez

et al. 2017

IJMS

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Digestive system cancers—those of the esophagus, stomach, small intestine, colon-rectum, liver, and pancreas—are highly related to genetics and lifestyle. Most are considered highly mortal due to the frequency of late diagnosis, usually in advanced stages, caused by the absence of symptoms or masked by other pathologies. Different tools are being investigated in the search of a more precise diagnosis and treatment. Plant lectins have been studied because of their ability to recognize and bind to carbohydrates, exerting a variety of biological activities on animal cells, including anticancer activities. The present report integrates existing information on the activity of plant lectins on various types of digestive system cancers, and surveys the current state of research into their properties for diagnosis and selective treatment.

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Purification and cloning of lectin that induce cell apoptosis from Allium chinense

Cited by 14 publications

References 24 publications

Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

Morus alba Leaf Lectin (MLL) Sensitizes MCF-7 Cells to Anoikis by Inhibiting Fibronectin Mediated Integrin-FAK Signaling through Ras and Activation of P38 MAPK

Plant Lectins as Medical Tools against Digestive System Cancers

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