Purification and characterization of α-l-arabinofuranosidase from Arthrobacter sp. MTCC 5214 in solid-state fermentation

Khandeparker, Rakhee; Numan, Mondher Th.; Mukherjee, Bishwajit; Satwekar, Abhijeet; Bhosle, N.B.

doi:10.1016/j.procbio.2008.02.014

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…Relatively very few attempts have been reported on the production of these enzymes under SSF. Khandeparker et al [28] reported wheat bran was the best substrate for α-L-arabinofuranosidase production by Arthrobacter sp. MTCC 5214 under SSF.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, it was proved that the statistical optimization increased α-L-arabinofuranosidase production by 2.34-fold as compared to their initial production which was 9.45 U/g (1.35 U/ml) before statistical optimization. Khandeparker [28] reported a maximum 3 U/g of α-Larabinofuranosidase production after 120 h of incubation by Arthrobacter sp. MTCC 5214 under SSF.…”

Section: Validation Of the Experimental Modelmentioning

confidence: 99%

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Improved yield of α-L-arabinofuranosidase by newly isolated Aspergillus niger ADH-11 and synergistic effect of crude enzyme on saccharification of maize stover

Patel

Chapla

Divecha

et al. 2015

Bioresour. Bioprocess.

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Background: In the view of depleting resources and ever-increasing price of crude oil, there is an urge for the development of alternative sources to solve the issue of fuel in the coming years. Lignocellulosic biomass is considered to be the most potential alternative resources for fossil fuel. Bioconversion of cellulosic and hemicellulosic components into fermentable sugars is the key step in producing fuel ethanol from lignocellulose. The enzymatic hydrolysis of lignocellulosic biomass needs a highly balanced composition of cellulases and hemicellulases. Commercial enzymes are usually poor in accessory hemicellulolytic enzymes like α-L-arabinofuranosidase. The addition of such accessory enzymes in combination with cellulase or hemicellulase plays a vital role in improving the total yield of fuel ethanol by enhancing the saccharification yield. Results: The newly isolated fungal strain Aspergillus niger ADH-11 produced a maximum of 22.14 U/g of α-L-arabinofuranosidase under solid-state fermentation using wheat bran as the substrate and modified Mandels-Weber medium at 30°C after 180 h of incubation. The optimization of various fermentation parameters was performed by response surface methodology employing Plackett-Burman design followed by Box-Behnken design. The yield of α-L-arabinofuranosidase was enhanced by 2.34-fold after executing statistical optimization of various fermentative parameters. Crude α-L-arabinofuranosidase was found to be highly stable for 3 h at its optimum temperature (55°C) and pH (4.0). The assessment of the crude enzyme extract in saccharification of alkali-treated maize stover revealed that the supplementation of crude α-L-arabinofuranosidase to commercial cellulase and crude xylanase mixture increased the saccharification yield up to 730 mg/g of maize stover.

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Section: Resultsmentioning

confidence: 99%

Section: Validation Of the Experimental Modelmentioning

confidence: 99%

Improved yield of α-L-arabinofuranosidase by newly isolated Aspergillus niger ADH-11 and synergistic effect of crude enzyme on saccharification of maize stover

Patel

Chapla

Divecha

et al. 2015

Bioresour. Bioprocess.

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show abstract

“…The inhibitory effect of Hg 2+ on AFases activities has been reported [96][97][98][99]. Besides Hg 2+ , Ag 2+ , and Pb 2+ are mixed inhibitors, which do not bind to the active site, but to another region of the enzyme, and thus do not interfere with substrate binding to the catalytic site.…”

Section: Metal Ions Associate To Hemicellulases Activitiesmentioning

confidence: 93%

Effect of Metal Ions, Chemical Agents and Organic Compounds on Lignocellulolytic Enzymes Activities

Pereira¹,

Giese²,

Moretti³

et al. 2017

Enzyme Inhibitors and Activators

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“…Collected pellet was used for extraction of total protein. Cellular protein content was used as parameter to determine bacterial growth as per method described by Khandeparker et al, (2008). In brief, pellet was treated with 0.1N NaOH and kept in boiling water bath for 30 min, this was done to extract total proteins, further sample was cooled and neutralized by adding equal volume of 0.1N HCl.…”

Section: Growth Curve and Xylanase Productionmentioning

confidence: 99%

Isolation, Purification and Characterization of Xylanase produced by Bacillus sp. NIORKP76 strain under solid state fermentation and its application in saccharification of various agro-residues into fermentable sugars

Parab

Khandeparker

Amberkar

2023

Preprint

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Microbial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. Increasing demand for these enzymes versus inadequate supply makes these biomolecules a value-added product. The current study is focused on xylanase from bacterial isolate identified as Bacillus sp. NIORKP76. The bacterial isolate used in this study showed ability to produce xylanase on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth media supplemented with phosphate, NaCl and NH4Cl concentration of 64 mM, 15 mg/mL and 0.3 mg/mL respectively. The maximum xylanase titer volume was obtained with 1:3 substrate to moisture ratio (w/v). Using optimized conditions maximum xylanase production in wheat bran was achieved in 72 h at room temperature 28 ±2°C. Xylanase exhibited pH optima of 8.0 and retained 92% of its residual activity after 24h incubation period at pH 8.0, thus proving its high stability at alkaline pH. Xylanase displayed optimum activity at 60°C. Xylanase stability at 30°C and 40 °C remained unhindered even after 12h of incubation period. The xylanase isolated in this study was purified up to homogeneity and its molecular weight was found to be ~28kDa. Xylanase produced by Bacillus sp. NIORKP76 strain was found to have essential qualities required for saccharification of various agro-residues to generate fermentable sugars which can be a raw-material for biofuel production. Wheat bran with heat pre-treatment was found to be par excellence agro-waste as compared to other heat pre-treated and untreated lignocellulosic agro-wastes in production of fermentable reducing sugars. 141mg/g fermentable sugars were whipped up when reaction mixture of pre-treated wheat bran with 5U/g partially purified xylanase incubated at 40°C for 8h, which was found to be best results among all agro-residues studied.

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Purification and characterization of α-l-arabinofuranosidase from Arthrobacter sp. MTCC 5214 in solid-state fermentation

Cited by 10 publications

References 44 publications

Improved yield of α-L-arabinofuranosidase by newly isolated Aspergillus niger ADH-11 and synergistic effect of crude enzyme on saccharification of maize stover

Improved yield of α-L-arabinofuranosidase by newly isolated Aspergillus niger ADH-11 and synergistic effect of crude enzyme on saccharification of maize stover

Effect of Metal Ions, Chemical Agents and Organic Compounds on Lignocellulolytic Enzymes Activities

Isolation, Purification and Characterization of Xylanase produced by Bacillus sp. NIORKP76 strain under solid state fermentation and its application in saccharification of various agro-residues into fermentable sugars

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