Purification and characterization of α- and β-benincasins, arginine/glutamate-rich peptides with translation-inhibiting activity from wax gourd seeds

Ng, T.B.; Parkash, A.; Tso, Wung-Wai

doi:10.1016/s0196-9781(02)00271-1

Cited by 20 publications

(4 citation statements)

References 20 publications

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“…For example, luffacylin, luffangulin, and luffin P1 were isolated from the seeds of Luffa cylindrical , and the molecular weights of luffacylin, luffangulin, and luffin P1 are only 7.8, 5.6, and 5.226 kDa, respectively ( Parkash et al, 2002a ; Wang and Ng, 2002 ; Li et al, 2003b ). Various low molecular weight type I RIPs were isolated and purified from Cucurbitaceae plants, such as α-benincasin and β-benincasin with molecular weights of 12 kDa, which have been isolated and purified from the seeds of Benincasa hispida ( Ng et al, 2003 ). In addition, S-trichokirin, trichokirin S1, and trichosanthrip isolated and purified from the seeds of the Trichosanthes kirilowii were also low molecular weight type I RIPs.…”

Section: Introductionmentioning

confidence: 99%

“…It demonstrates antifungal activity against Fusarium oxysporum and Mycosphaerella arachidicola in vivo ( Parkash et al, 2002a ). Two 11 kDa have been proteins are isolated from winter melon ( Benincasa hispida ) seeds ( Ng et al, 2003 ). They exhibited antifungal activity toward Coprinus comatus and Physalospora piricola ( Ng et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

“…Two 11 kDa have been proteins are isolated from winter melon ( Benincasa hispida ) seeds ( Ng et al, 2003 ). They exhibited antifungal activity toward Coprinus comatus and Physalospora piricola ( Ng et al, 2003 ). The isolated TRIP was showed to inhibit several fungi pathogens, including Trichoderma reesei, Cytospora canker, Fusarium oxysporum , and Cochliobolus heterostrophus ( Sharma et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

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The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

et al. 2018

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Ribosome-inactivating proteins (RIPs) are toxic N-glycosidases that depurinate eukaryotic and prokaryotic rRNAs, thereby arresting protein synthesis during translation. RIPs are widely found in various plant species and within different tissues. It is demonstrated in vitro and in transgenic plants that RIPs have been connected to defense by antifungal, antibacterial, antiviral, and insecticidal activities. However, the mechanism of these effects is still not completely clear. There are a number of reviews of RIPs. However, there are no reviews on the biological functions of RIPs in defense against pathogens and insect pests. Therefore, in this report, we focused on the effect of RIPs from plants in defense against pathogens and insect pest attacks. First, we summarize the three different types of RIPs based on their physical properties. RIPs are generally distributed in plants. Then, we discuss the distribution of RIPs that are found in various plant species and in fungi, bacteria, algae, and animals. Various RIPs have shown unique bioactive properties including antibacterial, antifungal, antiviral, and insecticidal activity. Finally, we divided the discussion into the biological roles of RIPs in defense against bacteria, fungi, viruses, and insects. This review is focused on the role of plant RIPs in defense against bacteria, fungi, viruses, and insect attacks. The role of plant RIPs in defense against pathogens and insects is being comprehended currently. Future study utilizing transgenic technology approaches to study the mechanisms of RIPs will undoubtedly generate a better comprehending of the role of plant RIPs in defense against pathogens and insects. Discovering additional crosstalk mechanisms between RIPs and phytohormones or reactive oxygen species (ROS) against pathogen and insect infections will be a significant subject in the field of biotic stress study. These studies are helpful in revealing significance of genetic control that can be beneficial to engineer crops tolerance to biotic stress.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

et al. 2018

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“…In addition, peptides of around 10 kDa obtained from the cucurbits Cucumis melo L. and Benincasa hispida Cogn. have been reported as RIPs with antifungal activity [ 89 , 101 ]. On the other hand, the ribotoxin α-sarcin [ 108 ] and the RLPs ageritin [ 114 , 115 ] and eryngitins 3 and 4 [ 116 ] have also shown antifungal activity, indicating that, although never used for that purpose, these proteins could also be tools to defend crops against fungal diseases.…”

Section: Inhibition Of Fungal Growth By Ripsmentioning

confidence: 99%

Antifungal Activity of Ribosome-Inactivating Proteins

Iglesias,

Citores,

Gay

et al. 2024

Toxins

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The control of crop diseases caused by fungi remains a major problem and there is a need to find effective fungicides that are environmentally friendly. Plants are an excellent source for this purpose because they have developed defense mechanisms to cope with fungal infections. Among the plant proteins that play a role in defense are ribosome-inactivating proteins (RIPs), enzymes obtained mainly from angiosperms that, in addition to inactivating ribosomes, have been studied as antiviral, fungicidal, and insecticidal proteins. In this review, we summarize and discuss the potential use of RIPs (and other proteins with similar activity) as antifungal agents, with special emphasis on RIP/fungus specificity, possible mechanisms of antifungal action, and the use of RIP genes to obtain fungus-resistant transgenic plants. It also highlights the fact that these proteins also have antiviral and insecticidal activity, which makes them very versatile tools for crop protection.

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Ribosome Inactivating Proteins and Apoptosis

Sikriwal

Batra

2010

Toxic Plant Proteins

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Purification and characterization of α- and β-benincasins, arginine/glutamate-rich peptides with translation-inhibiting activity from wax gourd seeds

Cited by 20 publications

References 20 publications

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

Antifungal Activity of Ribosome-Inactivating Proteins

Ribosome Inactivating Proteins and Apoptosis

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