Purification and characterization of two thermostable laccases from Pycnoporus sanguineus and potential role in degradation of endocrine disrupting chemicals

Ramírez-Cavazos, Leticia I.; Junghanns, Charles; Ornelas‐Soto, Nancy; Cárdenas‐Chávez, Diana L.; Hernández-Luna, Carlos E.; Demarche, Philippe; Enaud, Estelle; García-Morales, Raúl; Agathos, Spiros N.; Parra‐Saldívar, Roberto

doi:10.1016/j.molcatb.2014.06.006

Cited by 135 publications

(88 citation statements)

References 51 publications

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“…3d). Earlier, two thermostable laccase isozymes have been reported from Pycnoporus sanguineus by using ultrafiltration, ion exchange and hydrophobic interaction chromatography [38]. High throughput techniques currently used to purify proteins are sometimes cost intensive, time consuming and practically impossible.…”

Section: Purification and Characterization Of Isozymesmentioning

confidence: 99%

Laccase isozymes from Ganoderma lucidum MDU-7: Isolation, characterization, catalytic properties and differential role during oxidative stress

Kumar

Sharma

Kumar

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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Section: Purification and Characterization Of Isozymesmentioning

confidence: 99%

Laccase isozymes from Ganoderma lucidum MDU-7: Isolation, characterization, catalytic properties and differential role during oxidative stress

Kumar

Sharma

Kumar

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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“…Cerrena unicolor (Wang et al, 2017), Lentinula edodes (Nagai et al, 2003), Pycnoporus sanguineus (Ramírez-Cavazos et al, 2014), Trametes orientalis (Zheng et al, 2017), Trichoderma harzianum (El Monssef et al, 2016;Bagewadi et al, 2017), Pleurotus ostreatus (Othman et al, 2014;El-Batal et al, 2015), Pleurotus sajor-caju (Fokina et al, 2016), Pleurotus pulmonarius (Marques de Souza and Peralta, 2003;Behrens et al, 2017), Pleurotus sapidus (Schwienheer et al, 2015), Pleurotus eryngii (Loi et al, 2018), Pleurotus ferulae (Ding et al, 2014;Wang et al, 2015), and Pleurotus djamor (Salmones and Mata 2015) have been purified, characterized and studied in detail by many authors. However, laccase produced by P. tuber-regium has never been studied in detail, even though a previous study demonstrated the versatility of this fungus (Isikhuemhen and Nerud, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Its basidiocarps develop from underground sclerotia, and emerge from the soil when the atmospheric conditions are favorable (Okhuoya and Okogbo, 1990). Although a number of studies are available on the laccases obtained from white-rot fungi and their applications (Nagai et al, 2003;Ramírez-Cavazos et al, 2014;Pandey et al, 2018), there is only one preliminary investigation on the ligninolytic enzymes from P. tuber-regium reported so far (Isikhuemhen and Nerud, 1999). Laccase produced by P. tuber-regium has been poorly studied, and the characterization as well as purification of laccase from this fungus has received no attention.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of laccase from Pleurotus tuber-regium and its application in dye decolourization

Bamigboye¹,

Oloke²,

Olaogun³

et al. 2019

bta

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Pleurotus tuber-regium is a unique sclerotium-forming white rot fungus. It is edible and has medicinal value and a high potentials of application in various industries. However, to date, there is no information on the production of laccase by this fungus, the purification and determination of decolourization potential of laccase have also not been reported. In this study, purification of laccase from P. tuber-regium is demonstrated for the first time. Laccase was purified from the submerged culture of P. tuber-regium using ammonium sulfate precipitation, ion-exchange chromatography and gel filtration chromatography. The molecular weight of laccase was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified laccase was characterized to determine its optimum pH and temperature, substrate specificity, Michaelis-Menten constant, and inhibitors. The decolourization potential of the crude laccase was evaluated using Congo red, trypan blue, textile dyes and textile effluent. The purified laccase had a molecular weight of 52 kDa, optimum pH and temperature were 4 and 60EC, respectively. The best substrate for laccase was found to be 2,2N-azino-bis (3-ethylbenzothiazoline-6-sulfonate), with a Michaelis-Menten constant (K m ) of 7.82 μM. Laccase activity was mildly inhibited by ethylenediamine tetraacetic acid (EDTA) and L-cysteine, but strongly inhibited by sodium azide. The activity of the purified laccase was enhanced by more than two fold by Na + , Ca 2+ and Ba 2+ , and slightly enhanced by Hg 2+ and Mn 2+ . The crude laccase was able to efficiently decolourize trypan blue and Congo red dyes and red and blue textile dyes. Notably, on solid agar, trypan blue was completely degraded by intracellular laccase.

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“…Therefore, enzymatic treatment process, involving the use of ligninolytic oxidative enzymes for EDC removal, has recently attracted attention as an environmentally friendly alternative. The potential of some LEs including laccase, MnP, and VP for efficient removal of EDC in water has recently been reported (Diano & Mita, ; Garcia‐Morales et al., ; Ramírez‐Cavazos et al., ; Taboada‐Puig et al., ; Touahar et al., ; Wen, Jia, & Li, , ; Zhang & Geissen, ).…”

Section: Potential Of Les In the Elimination Of Edc In Wastewatermentioning

confidence: 99%

“…Therefore, enzymatic treatment process, involving the use of ligni- Garcia-Morales et al, 2015;Ramírez-Cavazos et al, 2014;Taboada-Puig et al, 2011;Touahar et al, 2014;Wen, Jia, & Li, 2009, 2010Zhang & Geissen, 2010).…”

Section: P Otential Of Le S In the Eliminati On Of Ed C In Wa S Te mentioning

confidence: 99%

Ligninolytic enzymes: Versatile biocatalysts for the elimination of endocrine‐disrupting chemicals in wastewater

et al. 2018

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Direct municipal wastewater effluent discharge from treatment plants has been identified as the major source of endocrine‐disrupting chemicals (EDC) in freshwaters. Consequently, efficient elimination of EDC in wastewater is significant to good water quality. However, conventional wastewater treatment approaches have been deficient in the complete removal of these contaminants. Hence, the exploration of new and more efficient methods for elimination of EDC in wastewater is imperative. Enzymatic treatment approach has been suggested as a suitable option. Nonetheless, ligninolytic enzymes seem to be the most promising group of enzymes for EDC elimination, perhaps, owing to their unique catalytic properties and characteristic high redox potentials for oxidation of a wide spectrum of organic compounds. Therefore, this paper discusses the potential of some ligninolytic enzymes (laccase, manganese peroxidase, and versatile peroxidase) in the elimination of EDC in wastewater and proposes a new scheme of wastewater treatment process for EDC removal.

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Purification and characterization of two thermostable laccases from Pycnoporus sanguineus and potential role in degradation of endocrine disrupting chemicals

Cited by 135 publications

References 51 publications

Laccase isozymes from Ganoderma lucidum MDU-7: Isolation, characterization, catalytic properties and differential role during oxidative stress

Laccase isozymes from Ganoderma lucidum MDU-7: Isolation, characterization, catalytic properties and differential role during oxidative stress

Purification and characterization of laccase from Pleurotus tuber-regium and its application in dye decolourization

Ligninolytic enzymes: Versatile biocatalysts for the elimination of endocrine‐disrupting chemicals in wastewater

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