Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotán, Atacama Desert

Contreras, Francisca; Amenábar, Maximiliano J.; Blamey, Jenny M.

doi:10.1007/s00792-020-01210-z

Cited by 5 publications

(1 citation statement)

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“…In this regard, a recent study reported the characterization of two thermostable xylanases resistant to freeze-drying cycles from a halotolerant Bacillus sp. isolated from Salar de Ascotán, with proposed industrial applications [84]. Moreover, among our set of MAGs, we found several forming deep isolated branches within their respective lineages, with proposed taxonomic novelty at high taxonomic ranks.…”

Section: Discussionmentioning

confidence: 55%

Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascotán Microbiota, as Revealed by Metagenome-Assembled Genomes

Veloso,

Waldisperg,

Arros

et al. 2023

Microorganisms

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Salar de Ascotán is a high-altitude arsenic-rich salt flat exposed to high ultraviolet radiation in the Atacama Desert, Chile. It hosts unique endemic flora and fauna and is an essential habitat for migratory birds, making it an important site for conservation and protection. However, there is limited information on the resident microbiota’s diversity, genomic features, metabolic potential, and molecular mechanisms that enable it to thrive in this extreme environment. We used long- and short-read metagenomics to investigate the microbial communities in Ascotán’s water, sediment, and soil. Bacteria predominated, mainly Pseudomonadota, Acidobacteriota, and Bacteroidota, with a remarkable diversity of archaea in the soil. Following hybrid assembly, we recovered high-quality bacterial (101) and archaeal (6) metagenome-assembled genomes (MAGs), including representatives of two putative novel families of Patescibacteria and Pseudomonadota and two novel orders from the archaeal classes Halobacteriota and Thermoplasmata. We found different metabolic capabilities across distinct lineages and a widespread presence of genes related to stress response, DNA repair, and resistance to arsenic and other metals. These results highlight the remarkable diversity and taxonomic novelty of the Salar de Ascotán microbiota and its rich functional repertoire, making it able to resist different harsh conditions. The highly complete MAGs described here could serve future studies and bioprospection efforts focused on salt flat extremophiles, and contribute to enriching databases with microbial genome data from underrepresented regions of our planet.

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Section: Discussionmentioning

confidence: 55%