Purification and Characterization of Three Galactose Specific Lectins from Sajna (Moringa Oleifera L.) Leaves

Abstract

Three lectins designated as SLL‐1, SLL‐2 and SLL‐3 were purified from small sized Sajna (Moringa oleifera L.) leaves by gel filtration of 100% ammonium sulfate saturated crude protein extract on Sephadex G‐75 followed by ion‐exchange chromatography on DEAE and affinity chromatography on Sepharose‐4B. The molecular weight of the lectins SLL‐1, SLL‐2 and SLL‐3 were 1,55,000, 1,15,000 and 85,000, respectively, as determined by gel filtration on Sephadex G‐150 and 1,60,000; 1,20,000 and 85,500, respectively, by SDS‐polyacrylamide slab gel electrophoresis. SLL‐1 and SLL‐2 are dimer in nature held together by disulfide bond (s), while SLL‐3 is a monomer. The lectins agglutinated specifically rat red blood cells and the agglutination was inhibited specifically by methyl‐α‐D‐galactopyranoside, methyl‐β‐D‐galactopyranoside and D‐galactose. The lectins SLL‐1, SLL‐2 and SLL‐3 contain 3.9, 3.4 and 2.8% neutral sugar, respectively, and the sugar compositions were glucose for SLL‐1, mannose for SLL‐2 and SLL‐3 contained either N‐acetyl‐D‐glucosamine or N‐acetyl‐D‐galactosamine or both. The lectins exhibited cytotoxicity in brine shrimp lethality bioassay.

Milk casein modification in response to dual oxidative enzymatic system

“…Extraction of peroxidase from the fresh leaves (0.5 g/ml) of moringa (Moringa oleifera) was performed according to Khatun et al (2012) using 100 mM Tris-HCl pH 7.5 with 0.1% PVP as an extraction buffer. After macerating for 24 h/5 °C, the extract was filtrated through gauze and centrifugation (5000 rpm, 20 min, 5 °C), the recovered fractions were pooled and considered as a crude enzymatic extract, and then peroxidase activity and protein content were determined.…”

Effect of autoclaving-time treatment on physicochemical, antioxidant properties and shelf-life prediction of Indonesian instant cassava leaves porridge

“…Calcium is an essential micronutrient for linear growth early (Gupta et al, 2018). As a result, insufficient calcium intake in toddlers impairs the basic structure of the bones, leading to stunting or other growth problems (Khatun et al, 2012;Fatima et al, 2014). The essential amino acids are amino acids that the body can't synthesize.…”