Purification and characterization of thermostable chitinase from a novel S. maltophilia strain

Abstract

Aims: The presents study examines the purification and characterization of a chitinase from S. maltophilia SJ602 strain isolated from a soil sample collected from Jamia Hamdard, New Delhi. Methodology and Results: The purification steps included chitin affinity using colloidal chitin as the affinity matrix and column chromatography using Sephadex G-100. The chitinase was purified to 5.85 fold having a yield of 17%. The molecular weight of the chitinase was found to be around 29 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The pH and temperature optima of the purified chitinase were found to be at pH 5.5 and 60 °C, respectively. Conclusion, Significance and Impact of the study: Besides showing a significant yield, the enzyme has a high thermal stability which has its applicability in the recycling of chitin waste.

Adsorption and recovery characteristics of phosphorylated sawdust bead for indium(III) in industrial wastewater

“…where k f is the constant related to the adsorprion capacity and 1/n is an empirical parameter respect to the adsorption intensity which varies with the heterogeneity of material [25]. The estimated values of 1/n and K f for indium(III) were 0.337 and 1.246, respectively, and correlation coefficient (r 2 ) was 0.964, as shown in Fig.…”

Hydrolysis of chitozan with an enzyme complex from Myceliophthora sp.

“…Colloid chitin was obtained using the method described in [18]. Chitin/chitosan with a DD of 42% was obtained by acetylation of chi tosan with a MM of 190-310 kDa and a DD of 75-85% "Sigma" (United States) using the modified method described earlier [19].…”

Polsko-postkolonialne podobieństwa? Recepcja tłumaczonej literatury postkolonialnej w Polsce (1970–2010)

“…Fakt, że Polacy stali się liczną grupą imigrantów w tym samym społeczeństwie, tworzy punkt styczny polskich i postkolonialnych doświadczeń. Mohsin Hamid (2008) sugeruje, że przynależność rasowa czy etniczna pozostaje istotną kategorią, kiedy mówi, że dzięki wschodnim Europejczykom "my, imigranci", to w dużej mierze grupa białych. Nikita Lalwani (2008) z kolei opowiada o fazie adaptacji, po której nowo przybyli imigranci przestają dzielić ludzi na swoich i obcych.…”