Purification and characterization of the (1→3)-β-glucanases fromAcremoniumsp. IMI 383068

Abstract

Three extracellular (1C3)-L-glucanases were purified from the fungus Acremonium sp. IMI 383068. Higher activities were unexpectedly obtained with pustulan, a (1C6)-L-glucan as carbon source, than when grown with laminarin, a (1C3)-L-glucan. Preliminary evidence suggests that these enzymes are not constitutive, but are inducible, and that their synthesis is repressed by glucose. All three had the same molecular masses, similar pH and temperature optima and none were glycosylated. They all appeared to have an exo-hydrolytic mode of substrate attack. N-terminal amino acid sequence data indicate that substantial post-translational modification of these had occurred, and that while two may be encoded by the same gene, the third may be genetically different.

Studies on the microbial transformation of androst-1,4-dien-3,17-dione with Acremonium strictum

“…The genus is well known for producing numerous important enzymes including alkaline protease [4], isopenicillin N synthetase [5], glucooligosaccharide oxidase [6], protease [7], ascorbate oxidase [8], phenol oxidase [9] and -glucanases [10]. Xenovulene A, a novel GABA-benzodiazepine receptor binding compound [11]; acremonol and acremodiol, two new fungal bislactones [12]; acremostatins A, B and C, three new lipoaminopeptides [13]; UCS 1025 A and B, new antitumor antibiotics [14] and the polyketide-derived antibiotics [15] are some new secondary metabolites isolated from the genus belonging to Acremonium.…”

Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01

“…Both Exo (1→3)-and (1→6)-β-glucanases were purified from culture media of Acremonium sp. IMI 383068 grown on pustulan as substrate, as described by Jayus et al (2001) and Jayus et al (2002), and purified with FPLC (AKTA, Amersham Pharmacia Biotech, Sweden) using an anion exchange column (Sigma) as described by Jayus et al (2004). EPS hydrolysis was performed with each enzyme in sodium acetate buffer (50 mM pH 5.0) containing the EPS (2 mg/mL) as a substrate at 40 o C for 30 min.…”

Effectiveness of Immunotherapies from Oyster Mushroom (Pleurotus species) in the Management of Immunocompromised Patients

“…About half the mass of the fungal cell wall consists of β-glucans [78,79,77], with many of them excreted into the growth medium, making their recovery, purification and chemical characterization much simpler [78,76] and they are referred to as extracellular metabolite . β-glucans homeostasis is maintained by β-glucan synthases and β-(1-3) or β-(1-6)-glucanase activities [80,81]. Some fungal β-glucans that markedly stimulate the immune system, offer protection from attack by pathogenic microbes and from harmful effects of environmental toxins and carcinogens [82,83].…”