Purification and characterization of the NAD(P)H-nitroreductase for the catabolism of 2,4,6-trinitrotoluene (TNT) inPseudomonas sp. HK-6

Kahng, Hyung-Yeel; Lee, Bheong-Uk; Cho, Yun-Seok; Oh, Keun-Yeob

doi:10.1007/bf02931067

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…DL6 [12] and Pseudomonas sp. HK 6 [10]. In contrast, the nitrore ductase purified from S. mirabilis DUT001 was active as a homodimer of 68 kDa [11].…”

Section: Resultsmentioning

confidence: 52%

“…Similarly, the pH optimum at 7.5 was revealed for the nitroreductase purified from Pseudomonas sp. HK 6 [10] and S. mirabilis DUT001 [11]. A higher optimum pH of 9.5 and a low optimum of 5.5-6.5 were observed for nitrogenases isolated from Aquiflexum sp.…”

Section: Resultsmentioning

confidence: 90%

“…The nitroreductases purified from Pseudomonas sp. HK 6 [10] and C. acetobutylicum ATCC 824 [9] were also active at 30°C. Yang et al [11] and Misal et al [12] reported bacterial nitroreductases that are active at 40°C.…”

Section: Resultsmentioning

confidence: 95%

“…A low specific activity of 1.47 U/mg with 10.1 fold increase was observed for the purification of NAD(P)H dependent nitroreductase of Pseudomonas sp. HK 6 [10]. Yang et al [11] reported 33.79 fold purification for a NADH dependent nitroreductase from Streptomyces mirabilis DUT001 with specific activity of 1151.5 U/mg and 32% yield.…”

Section: Resultsmentioning

confidence: 97%

“…Nitroreductases are classified into oxygen sensitive and insensitive based on the behavior of nitroreductases towards oxygen [1]. Some reports for the purification and biochemical properties of the nitroreductases obtainable from bacterial sources are available [1,[4][5][6][7][8][9][10][11][12]. Ammonium sulfate is often used as a precipitating agent for nitroreductases, whereas ion exchange chro matography and/or gel filtration chromatography are used to purify various nitroreductases [5,8,11,12].…”

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confidence: 99%

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Purification and properties of pendimethalin nitroreductase from Bacillus circulans

More¹,

Tallur²,

Ninnekar

et al. 2015

Appl Biochem Microbiol

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A pendimethalin nitroreductase was purified from Bacillus circulans to 52 fold increase with a recovery of 21.1% and had a specific activity of 4.16 U/mg. The molecular weight was 27 kDa. The optimum pH and temperature were 7.5 and 35°C, respectively. Fe 2+ , Ca 2+ , Mg 2+ and Mn 2+ did not cause any effect on the enzyme activity, whereas Ag + , Hg 2+ significantly inhibited it. The pendimethalin nitroreductase from B. circulans required NADPH as cofactor. It was not affected by GSH, DTT, L Cys and β mercaptoethanol but inhibited by p hydroxymercuribenzoate, N ethylmaleimide, and iodoacetamide. EDTA, 1,10 phenan throline and α,α' dipyridyl had a moderate effect on the enzyme activity. The nitroreductase reduced all the tested nitroaromatic compounds but was more active towards pendimethalin and trifluralin. The K M and V max for the enzyme reaction using pendimethalin as a substrate were 4.35 μM and 620 μmoles mg -1 min -1 , respectively. The broad substrate specificity towards dinitroaniline herbicides suggested the enzyme to be used as a potent reducing agent of these toxic compounds. This is the first report for the purification and charac terization of pendimethalin nitroreductase from any bacterial or fungal species.

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“…DL6 [12] and Pseudomonas sp. HK 6 [10]. In contrast, the nitrore ductase purified from S. mirabilis DUT001 was active as a homodimer of 68 kDa [11].…”

Section: Resultsmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 97%

mentioning

confidence: 99%

See 3 more Smart Citations

Purification and properties of pendimethalin nitroreductase from Bacillus circulans

More¹,

Tallur²,

Ninnekar

et al. 2015

Appl Biochem Microbiol

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Simultaneous degradation of nitroaromatic compounds TNT, RDX, atrazine, and simazine by Pseudomonas putida HK‐6 in bench‐scale bioreactors

Cho

Lee

2008

J of Chemical Tech & Biotech

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BACKGROUND: Environmental contamination by nitroaromatic compounds such as 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-s-triazine (RDX), atrazine, and/or simazine (TRAS) generated as waste from military and agricultural activities is a serious worldwide problem. Microbiological treatment of these compounds is an attractive method because many explosives and herbicides are biodegradable and the process can be made cost-effective. We explored the feasibility of using cultures of Pseudomonas putida HK-6 for simultaneous degradation of TRAS with the aim of microbial application in wastewater treatment in bench-scale bioreactors.

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Aerobic Biodegradation of 2,4,6-Trinitrotoluene (TNT) by Bacillus cereus İsolated from Contaminated Soil

et al. 2013

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In this study, biological degradation of 2,4,6-trinitrotoluene (TNT) which is very highly toxic environmentally and an explosive in nitroaromatic character was researched in minimal medium by Bacillus cereus isolated from North Atlantic Treaty Organization (NATO) TNT-contaminated soils. In contrast to most previous studies, the capability of this bacteria to transform in liquid medium containing TNT was investigated. During degradation, treatment of TNT was followed by high-performance liquid chromatography (HPLC) and achievement of degradation was calculated as percentage. At an initial concentration of 50 and 75 mg L(-1), TNT was degraded respectively 68 % and 77 % in 96 h. It transformed into 2,4-dinitrotoluene and 4-aminodinitrotoluene derivates, which could be detected as intermediate metabolites by using thin-layer chromatography and gas chromatography-mass spectrometry analyses. Release of nitrite and nitrate ions were searched by spectrophotometric analyses. Depending upon Meisenheimer complex, while nitrite production was observed, nitrate was detected in none of the cultures. Results of our study propose which environmental pollutant can be removed by using microorganisms that are indigenous to the contaminated site.

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Purification and characterization of the NAD(P)H-nitroreductase for the catabolism of 2,4,6-trinitrotoluene (TNT) inPseudomonas sp. HK-6

Cited by 10 publications

References 25 publications

Purification and properties of pendimethalin nitroreductase from Bacillus circulans

Purification and properties of pendimethalin nitroreductase from Bacillus circulans

Simultaneous degradation of nitroaromatic compounds TNT, RDX, atrazine, and simazine by Pseudomonas putida HK‐6 in bench‐scale bioreactors

Aerobic Biodegradation of 2,4,6-Trinitrotoluene (TNT) by Bacillus cereus İsolated from Contaminated Soil

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