Purification and characterization of Streptomyces albidoflavus antifungal components

Abstract

Chitinolytic strain Streptomyces albidoflavus was isolated from soil of the central region of Poland. Its identification was based on analysis of 16S rRNA gene sequence. The colloidal chitin was revealed as the finest substrate for the production of chitinases by S. albidoflavus. The enzyme catalyzed the hydrolysis of the disaccharide 4 methylumbelliferyl β D N,N',N'' triacetylchitotriose most efficiently and was, therefore, classified as an endochitinase. The chitinase of S. albidoflavus was purified by applying the two step proce dure: fractionation with ammonium sulphate and chitin affinity chromatography. The molecular weight of the purified enzyme determined by SDS PAGE was approximately 50 kDa. The enzyme was characterised as thermostable during 180 min of preincubation at the temperature of 35°C and 40°C. The activity of the enzyme was strongly inhibited in the presence of Hg 2+ and Mn 2+ ions, SDS but stabilized by Ca 2+ and Mg 2+ ions. Both purified and crude chitinases from S. albidoflavus inhibited the development of fungal phytopatho gens. Purified chitinase inhibited the growth of Alternaria alternata, Fusarium culmorum, Fusarium oxysporum and Botrytis cinerea. Additionally, the crude chitinase inhibited the growth of Fusarium solani.

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

“…The genus Streptomyces has been implicated over the years as a contraption of several metabolites and biomolecules, including hydrolytic enzymes [21,33]. However there has been scant report on hemicellulolytic potential of Streptomyces albidoflavus [34,35]. Microbial hydrolytic enzyme production is a multivariable controlled process involving the composition and microbial cell culture conditions.…”

Bioleaching of heavy metals from printed circuit board (PCB) by Streptomyces albidoflavus TN10 isolated from insect nest

“…Previously, several authors have reported the bioleaching potential of Streptomyces sp. isolated from different ecosystem ex (Brzezinskaa et al 2013;Long et al 2018;Daboor et al 2014;El Baz et al 2015). However, no previous evidence has been reported on bioleaching of heavy metals in printed circuit boards by Streptomyces albidoflavus.…”

Microbial Degradation of Lobster Shells to Extract Chitin Derivatives for Plant Disease Management

“…Chitinase obtained from S223 purified by affinity chromatography resulted in 3.2-fold purification at the final stage ( Table 1 ) and its molecular weight was found to be ∼30kDa ( Figure 3B ). Brzezinska et al (2013) purified chitinase up to 3.9-fold from S. albidoflavus and Mukherjee and Sen (2006) obtained a 3.19-fold purified chitinase from S. venezulae P10. Many researches have reported the molecular weight of purified chitinase from Streptomyces species was between 20 and 71 kDa (Joo, 2005; Han et al, 2009).…”

“…Chitinase was purified by affinity chromatography with colloidal chitin as substrate (Escott et al, 1998). The samples were concentrated using Amicon PM30 (MWCO 30kDa) ultrafiltration centrifugal tubes (Brzezinska et al, 2013). At each purification step, chitinase activity was measured using a nitrophenol based chitinase assay kit (Sigma).…”