Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by the<i>Lactobacillus salivarius</i>SGL 03

Pidutti, Paolo; Federici, Federica; Brandi, Jessica; Laura, Manna; Rizzi, Eleonora; Marini, U; Cecconi, Daniela

doi:10.1111/jam.13646

Cited by 34 publications

(30 citation statements)

References 36 publications

(64 reference statements)

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“…Despite a rather high bacterial colonization, the oral cavity is actually relatively resistant to infection due to innate host defense mechanisms and biologically active components of the saliva. Moreover, mutual interaction between nonspecific and specific microorganisms present in the mouth helps to create and maintain homeostasis [ 16 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, the presence of many pathogenic bacteria in the oral cavity, including red complex periopathogens, may cause disturbances in subgingival plaque homeostasis, which may lead to the development of severe and chronic periodontitis and various infections inducing inflammation in the oral cavity [ 2 , 5 , 17 , 18 , 27 ]. Red complex bacteria are very effective in blocking the body’s anti-inflammatory response to microbial attack.…”

Section: Introductionmentioning

confidence: 99%

“…They inhibit monocyte chemotaxis by blocking the expression of adhesion molecules in intercellular reactions, blocking the action of interleukin-8. Currently, it is believed that the lack of an appropriate anti-inflammatory response is the main etiopathogenetic factor in the development of periodontitis—which affects the majority of adult populations worldwide [ 6 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…L. salvarius SGL03 has a fairly fast cell life cycle with division every 10 min at 37 °C. It reaches the highest number of cells after 8–9 h in the medium [ 27 ]. In an in vitro study by Pidutti [ 27 ], it was shown that L. salivarius produces L27 and L30 bacteriocins, which inhibit the proliferation of S. pyogenes within 4–6 h after being added to the medium [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Ligilactobacillus salivarius and Other Natural Components against Anaerobic Periodontal Bacteria

Kucia¹,

Wietrak²,

Szymczak

et al. 2020

Molecules

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In this present study, the bacteriostatic effect of Salistat SGL03 and the Lactobacillus salivarius strain contained in it was investigated in adults in in vivo and in vitro tests on selected red complex bacteria living in the subgingival plaque, inducing a disease called periodontitis, i.e., chronic periodontitis. Untreated periodontitis can lead to the destruction of the gums, root cementum, periodontium, and alveolar bone. Anaerobic bacteria, called periopathogens or periodontopathogens, play a key role in the etiopathogenesis of periodontitis. The most important periopathogens of the oral microbiota are: Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and others. Our hypothesis was verified by taking swabs of scrapings from the surface of the teeth of female hygienists (volunteers) on full and selective growth media for L. salivarius. The sizes of the zones of growth inhibition of periopathogens on the media were measured before (in vitro) and after consumption (in vivo) of Salistat SGL03, based on the disk diffusion method, which is one of the methods of testing antibiotic resistance and drug susceptibility of pathogenic microorganisms. Additionally, each of the periopathogens analyzed by the reduction inoculation method, was treated with L. salivarius contained in the SGL03 preparation and incubated together in Petri dishes. The bacteriostatic activity of SGL03 preparation in selected periopathogens was also analyzed using the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests. The obtained results suggest the possibility of using the Salistat SGL03 dietary supplement in the prophylaxis and support of the treatment of periodontitis—already treated as a civilization disease.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Ligilactobacillus salivarius and Other Natural Components against Anaerobic Periodontal Bacteria

Kucia¹,

Wietrak²,

Szymczak

et al. 2020

Molecules

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“…The gel was stained with 50 mL of Coomassie brilliant blue R-250 (0.1 M) for 4 hours. The decolorizing solution was decolorized for 12 h until the electrophoresis band was clear [32].…”

Section: Molecular Weight Estimation With Sodium Dodecyl Sulfate-polymentioning

confidence: 99%

Novel Cytoplasmic Bacteriocin Compounds Derived from Staphylococcus epidermidis Selectively Kill Staphylococcus aureus, Including Methicillin-Resistant Staphylococcus aureus (MRSA)

et al. 2020

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Staphylococcus aureus (S. aureus) is one of the well-known agents causing atopic dermatitis (AD) in susceptible individuals, and Staphylococcus epidermidis (S. epidermidis) produces class I thermostable bacteriocins that can selectively kill S. aureus, suggesting protective roles against AD. There is a large need for developing precise therapies only to target S. aureus and not to harm the beneficial microbiome. On the agar well diffusion assay, live planktonic S. epidermidis showed clear zones of inhibition of S. aureus growth, but heat-killed cells and cell-free supernatants did not show this. These results would lead us to hypothesize that cytoplasmic bacteriocin from S. epidermidis will be a promising agent to inhibit S. aureus growth. Therefore, we have extracted a novel thermolabile cytoplasmic bacteriocin from S. epidermidis using trichloroactic acid (TCA)/acetone precipitation method after cell lysis with a SDS-containing buffer. These bacteriocin selectively exhibited antimicrobial activity against S. aureus and methicillin-resistance Staphylococcus aureus (MRSA), presenting no active actions against S. epidermidis, E. coli, and Salmonella Typhimurium. The extracted cytoplasmic bacteriocin compounds revealed several diffuse bands of approximately 40–70 kDa by SDS-PAGE. These findings suggest that these cytoplasmic bacteriocin compounds would be a great potential means for S. aureus growth inhibition and topical AD treatment.

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Identification of Antimicrobial Peptides from Macroalgae with Machine Learning

Caprani

Slattery

O’Keeffe

et al. 2020

Advances in Intelligent Systems and Computing

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Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by theLactobacillus salivariusSGL 03

Abstract: Our results provide the first insight into the extra-ribosomal activity of L27 and L30 secreted proteins of L. salivarius. This study demonstrated the capacity of L. salivarius SGL 03 to produce antimicrobial molecules and suggested this strain as a promising probiotic candidate.

Cited by 34 publications

References 36 publications

Effect of Ligilactobacillus salivarius and Other Natural Components against Anaerobic Periodontal Bacteria

Effect of Ligilactobacillus salivarius and Other Natural Components against Anaerobic Periodontal Bacteria

Novel Cytoplasmic Bacteriocin Compounds Derived from Staphylococcus epidermidis Selectively Kill Staphylococcus aureus, Including Methicillin-Resistant Staphylococcus aureus (MRSA)

Identification of Antimicrobial Peptides from Macroalgae with Machine Learning

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