Purification and Characterization of Recombinant Varicella-Zoster Virus Glycoprotein gpII, Secreted by Chinese Hamster Ovary Cells

Jacquet, Alain; Massaer, Marc; Haumont, Michèle; Houard, Sophie; Deleersnyder, Virginie; Place, Martine; Bollen, Alex; Jacobs, Paul

doi:10.1006/prep.1995.1012

Cited by 8 publications

(9 citation statements)

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“…Moreover, the sera were shown to neutralize VZV (Table 1). Mean antibody and neutralizing titers were comparable with those obtained after immunizations with adjuvanted native recgB (15). In contrast, vaccination with pNIV2086 encoding recgE, in the same conditions, induced a very weak antibody response, the titres being drastically lower than those elicited with adjuvanted recgE (13).…”

Section: Resultssupporting

confidence: 47%

“…The expression of recgB and recgE was under the control of the human cytomegaloviras (HCMV) immediate early gene promoter with the first intron A (IA), which is known to give a high level of transcription. The resulting expression plasmids pNIV 2085 and pNIV 2086, exactly encoding recombinant proteins identical to those expressed from CHO-K1 cells (13,15), are depicted in Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

“…The results were compared with those obtained after vaccinations with purified adjuvanted recgB and recgE expressed in CHO cells, for which strong neutralizing antibody responses were previously demonstrated (13,15). The study showed that mice immunization with a plasmid encoding a VZV gB soluble form elicited the production of neutralizing antibodies with titres comparable to those obtained with the corresponding recombinant protein.…”

Section: Introductionmentioning

confidence: 83%

“…Ten pg of each recombinant protein, purified from spent culture medium of recombinant CHO-K1 cell lines as previously described (13,15), was adsorbed to aluminium hydroxide and emulsified in MPL (proprietary formulation of SmithKline Beecham Biologicals, Rixensart, Belgium). When indicated, proteins were previously denatured for 5 minutes at 100°C in 2% SDS, 5% ß-mercaptoethanol, then diluted to a final 0.25% SDS concentration before formulation.…”

Section: Introductionmentioning

confidence: 99%

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Differential Neutralizing Antibody Responses to Varicella-Zoster Virus Glycoproteins B and E Following Naked DNA Immunization

Massaer¹,

Haumont²,

Garcia³

et al. 1999

Viral Immunology

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The only available vaccine against varicella-zoster virus (VZV) consists of the VZV-Oka attenuated but persistent virus strain. Development of a safer, subunit vaccine is therefore desirable. In this prospect, nucleic acid vaccines, expressing truncated forms of VZV glycoproteins B (recgB) and E (recgE) from which the anchor and the cytoplasmic domains were deleted, were used to immunize mice. Vaccination with recgB encoding plasmid elicited a strong and specific humoral immune response. Total IgG and neutralizing titres were comparable to those previously obtained by vaccination with purified and adjuvanted native recgB. In contrast, mice immunization with recgE encoding plasmid only induced a very weak immune response whereas we previously showed that vaccination with adjuvanted native or denatured recgE protein led to high neutralizing titres. The weakness of the immune response induced by recgE-encoding plasmid depended neither on the deletion of the anchor domain in the gE gene nor on the animal model. Analysis of antibody isotypes produced by plasmid immunizations revealed a response slightly dominated by IgG2a. Taken together, the data indicate that a VZV subunit vaccine based on adjuvanted recombinant glycoprotein E is more promising than a nucleic acid-based vaccine strategy. As regards recgB, both vaccination approaches might be appropriate.

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Section: Resultssupporting

confidence: 47%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential Neutralizing Antibody Responses to Varicella-Zoster Virus Glycoproteins B and E Following Naked DNA Immunization

Massaer¹,

Haumont²,

Garcia³

et al. 1999

Viral Immunology

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“…Moreover, a recombinant form of gB was shown to induce neutralizing antibodies [Massaer et al, 1993]. For the present study, purified recombinant gE and gB proteins [Jacquet et al, 1995;Haumont et al, 1996] were used to carry out depletion experiments in order to analyze further the potential of these proteins to stimulate the synthesis of neutralizing antibodies during the course of primary infection or reactivation.…”

Section: Introductionmentioning

confidence: 99%

Neutralizing antibody responses induced by varicella-zoster virus gE and gB glycoproteins following infection, reactivation or immunization

Haumont¹,

Jurdan²,

Kangro³

et al. 1997

J. Med. Virol.

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The purpose of this study was to compare the antibody responses to varicella-zoster virus (VZV) gE and gB after natural VZV infection and after vaccination with live attenuated OKA vaccine in order to determine the relative importance of these proteins as components of a subunit vaccine. Anti-VZV antibody titers determined by IFA were of the same order of magnitude in sera from individuals with a history of varicella and in vaccinated children but higher in individuals given booster vaccination. The titers of anti-gE and anti-gB antibodies were measured by ELISA using recombinant gE or gB as capture antigen. From these experiments, it appears that the ratio of anti-gE to anti-gB antibody is highly variable from one individual to another but relatively stable over a long period of time for a particular individual, even after a zoster episode. Neutralizing antibodies directed against gE or gB were also measured by subtracting the neutralization titers obtained before and after depletion of the specific antibodies on immobilized recombinant gE, gB, or both. This showed that, with respect to neutralization, anti-gE and anti-gB are equally prevalent in vaccinated children and that anti-gE is generally, but not always, predominant over anti-gB in VZV-infected individuals. Finally, antibodies to these two glycoproteins appear to be predominant among the neutralizing antibodies directed to other VZV antigens.

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Induction of cellular immunity to varicella‐zoster virus glycoproteins tested with pernasal coadministration of escherichia coli enterotoxin in mice

Tsuji

Shiraki

Sato

et al. 2003

Journal of Medical Virology

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A mutant of Escherichia coli enterotoxin promotes the induction of cellular immunity to a live varicella vaccine (the Oka strain) as a mucosal adjuvant in mice. An investigation was carried out to determine which of the purified glycoproteins of the virus among three induced cellular immunity with a single nasal administration. Spleen cells from mice immunized nasally with the vaccine and toxin produced interleukin-2 (IL-2) at the same level on restimulation in vitro with glycoprotein H: glycoprotein L (gH:gL), gB, and gE:gI, but not IL-4. The spleen cells from mice immunized with gH:gL, gB, or gE:gI and toxin produced IL-2 on restimulation with gH:gL, gB, or gE:gI, respectively, and the vaccine, but not IL-4. Immunization with gH:gL and the toxin showed increased thymidine uptake and production of IL-2 and interferon-gamma (IFN-gamma) of the spleen cells, but not IL-4, depending on the dose of gH:gL used for immunization and restimulation in vitro. Purified gE:gI and gB have been reported to be the strongest stimulators of cellular immunity to varicella upon subcutaneous injection and are useful as a subunit vaccine. All the glycoproteins tested are excellent stimulators of cellular immunity to the virus and itself on nasal co-immunization with the toxin.

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Purification and Characterization of Recombinant Varicella-Zoster Virus Glycoprotein gpII, Secreted by Chinese Hamster Ovary Cells

Cited by 8 publications

References 0 publications

Differential Neutralizing Antibody Responses to Varicella-Zoster Virus Glycoproteins B and E Following Naked DNA Immunization

Differential Neutralizing Antibody Responses to Varicella-Zoster Virus Glycoproteins B and E Following Naked DNA Immunization

Neutralizing antibody responses induced by varicella-zoster virus gE and gB glycoproteins following infection, reactivation or immunization

Induction of cellular immunity to varicella‐zoster virus glycoproteins tested with pernasal coadministration of escherichia coli enterotoxin in mice

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