Purification and characterization of pyocins frompseudomonas aeruginosa

Al-Shibib, A.; Al-Mudhaffar, Sami A.; Al-Ani, F. K.; Hassan, Farrukh

doi:10.1007/bf02922493

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…The final purification that was achieved after gel filtration chromatography was 8.2 folds with 2% recovery. These findings are in accordance with another study which also reported the purification of pyocin with an increase in the specific activity as well as purification up to 434-fold ( Al-Shibib et al ., 1985 ). In another study, the specific activity of the pyocin after every step of purification was increased and final recovery after chromatography using Sephacryl S 200 was observed to be 13% ( Sano and Kageyama, 1981 ).…”

Section: Resultssupporting

confidence: 93%

“…In the following step of conventional gel permeation chromatography, on Sephadex G-75 column, pyocin SA189 depicted the activity to reside in fraction 6, 7 and 8 and the chromatogram also showed a single peak of protein ( Figure 2 ). These findings correlate with the study, where pyocin was eluted from the CM Sephadex A-50 (pH 7.2) column as a single protein peak ( Al-Shibib et al ., 1985 ). Furthermore, Duport et al .…”

Section: Resultssupporting

confidence: 90%

“…Similar protease-resistant pyocins with 20–135 kg/mol. molecular masses have been reported earlier ( Al-Shibib et al ., 1985 ). In another study, R-type pyocin, resistant to all tested proteases, depicted a major band of about 30 kDa on SDS-PAGE ( Fontoura et al ., 2009 ).…”

Section: Resultssupporting

confidence: 79%

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Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

Naz¹,

Jabeen²,

Sohail³

et al. 2015

Braz. J. Microbiol.

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Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 90%

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Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

Naz¹,

Jabeen²,

Sohail³

et al. 2015

Braz. J. Microbiol.

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“…Interest in these particles continued to grow in the 1970s and 1980s as more R-pyocins became identified and purified ( Chen and Tai, 1972 ; Garcia Rodriguez and Saenz Gonzalez, 1972 ; Shinomiya, 1972 ; Govan, 1974a ; Al-Shibib et al, 1985 ; Al-Rubiee et al, 1988 ). Some of these R-pyocins were compared with each other and the tail fibers were identified as the component involved in their specificity ( Ohsumi et al, 1980 ; Kumazaki and Ishii, 1982 ).…”

Section: A Brief History Of Ecissmentioning

confidence: 99%

Evolutionary and ecological role of extracellular contractile injection systems: from threat to weapon

Heiman,

Vacheron,

Keel

2023

Front. Microbiol.

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Contractile injection systems (CISs) are phage tail-related structures that are encoded in many bacterial genomes. These devices encompass the cell-based type VI secretion systems (T6SSs) as well as extracellular CISs (eCISs). The eCISs comprise the R-tailocins produced by various bacterial species as well as related phage tail-like structures such as the antifeeding prophages (Afps) of Serratia entomophila, the Photorhabdus virulence cassettes (PVCs), and the metamorphosis-associated contractile structures (MACs) of Pseudoalteromonas luteoviolacea. These contractile structures are released into the extracellular environment upon suicidal lysis of the producer cell and play important roles in bacterial ecology and evolution. In this review, we specifically portray the eCISs with a focus on the R-tailocins, sketch the history of their discovery and provide insights into their evolution within the bacterial host, their structures and how they are assembled and released. We then highlight ecological and evolutionary roles of eCISs and conceptualize how they can influence and shape bacterial communities. Finally, we point to their potential for biotechnological applications in medicine and agriculture.

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Pyocyanin preparation fromPseudomonas aeruginosa isolated from heterogeneous clinical materials

et al. 1986

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Pure pyocyanin was prepared from 120 strains of Pseudomonas aeruginosa isolated from different clinical specimens. The modified method used increased the purification 425-fold. The spectra of pyocyanin of all strains were found to be identical with a single major peak at 363 nm. The only difference between these spectra appeared to be the level value absorbance. Strains recovered from different clinical specimens differ in their ability for pigment production. The strains isolated from urine produced higher amounts of pyocyanin than other isolates.

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Purification and characterization of pyocins frompseudomonas aeruginosa

Cited by 3 publications

References 8 publications

Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

Evolutionary and ecological role of extracellular contractile injection systems: from threat to weapon

Pyocyanin preparation fromPseudomonas aeruginosa isolated from heterogeneous clinical materials

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