Purification and Characterization of Protein Nanotubes Assembled from a Single Bacterial Microcompartment Shell Subunit

Noël, Christopher R.; Cai, Fei; Kerfeld, Cheryl A.

doi:10.1002/admi.201500295

Cited by 44 publications

(68 citation statements)

References 49 publications

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“…When shell proteins are expressed outside of their native cellular context they form a diverse array of aberrant structures, including filaments 63,120,150 , rosettes 19,107,120 and nanotubes 23,151,152 . These architectures provide an alternative use for shell proteins in the construction of modular scaffolds that could be used to enhance metabolic efficiency in vivo and in vitro 153 .…”

Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

“…New insights have emerged from the study of two non-natural BMC architectures: nanotubes and two-dimensional layers. Nanotubes can be formed by the expression of a single BMC-H proteins, PduA 151 or RmmH from Mycobacterium smegmatis 152 . For the latter, nanotube assembly is reversible and regulated in a concentration-dependent manner 152 .…”

Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

“…Nanotubes can be formed by the expression of a single BMC-H proteins, PduA 151 or RmmH from Mycobacterium smegmatis 152 . For the latter, nanotube assembly is reversible and regulated in a concentration-dependent manner 152 . Likewise, after their purification, the rosettes formed in vivo by the BMC-H protein of H. ochraceum roll-out into flat, single hexamer-thick sheets in which hexamers are uniformly oriented 19, 40 .…”

Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

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Bacterial microcompartments

et al. 2018

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Bacterial microcompartments (BMCs) are self-assembling organelles that consist of an enzymatic core that is encapsulated by a selectively permeable protein shell. The potential to form BMCs is widespread, found across the Kingdom Bacteria. BMCs have crucial roles in carbon dioxide fixation in autotrophs and the catabolism of organic substrates in heterotrophs. They contribute to the metabolic versatility of bacteria, providing a competitive advantage in specific environmental niches. Although BMCs were first visualized more than sixty years ago, it is mainly in the last decade that progress has been made in understanding their metabolic diversity and the structural basis of their assembly and function. This progress has not only heightened our understanding of their role in microbial metabolism but it is also beginning to enable their use in a variety of applications in synthetic biology. In this Review, we focus on recent insights into the structure, assembly, diversity and function of BMCs.

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Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

Section: Bacterial Microcompartments In Bioengineeringmentioning

confidence: 99%

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Bacterial microcompartments

et al. 2018

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“…It has been shown that it is possible to target fluorescent proteins to these bundles of filaments in vivo utilizing BMC targeting peptides [56]. A PduA homologue RmmH from Mycobacterium smegmatis was similarly found to form nanotubes however in this case the structures observed had a diameter of ~ 14 nm ( Figure 4B) [57]. Researchers have shown that mutations to residues at the hexamer-hexamer interface of PduA results in the formation of more sheet-like assemblies in vivo offering the opportunity to re-design a PduA based scaffold ( Figure 4C) [58].…”

Section: Engineering Bmc Componentsmentioning

confidence: 99%

Biotechnological Advances in Bacterial Microcompartment Technology

Lee

Palmer

Warren

2019

Trends in Biotechnology

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Bacterial microcompartments (BMCs) represent proteinaceous macromolecular nanobioreactors that are found in a broad range of bacteria, and which are associated with either anabolic or catabolic processes. They consist of a semipermeable outer shell that packages a central metabolic enzyme or pathway, providing both enhanced flux and protection against toxic intermediates. Recombinant production of BMCs has led to their repurposing with the incorporation of altogether new pathways. Deconstructing BMCs into their component parts has shown that some individual shell proteins self-associate into filaments that can be further modified into a cytoplasmic scaffold, or cytoscaffold, to which enzymes/proteins can be targeted. BMCs therefore represent a modular system that is highly suited for engineering biological systems for useful purposes.

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“…[26] Proteins are being explored for supramolecular chemistry applications and inspire the design of novel artificial nanomaterials fabricated by biotechnological, chemical, and computational expedients. [27,28] The access to ready-to-use protein assemblies with discrete structural patterns such as cages, [29] rings, [30] and tubes [31] is leading to promising results, for example, in magnetic resonance-based imaging. [32] As proteins, DNA has been used for similar purposes and DNA origami finds now interesting applications as scaffold for nanofabrication.…”

Section: Introductionmentioning

confidence: 99%

Bio‐Assisted Tailored Synthesis of Plasmonic Silver Nanorings and Site‐Selective Deposition on Graphene Arrays

Giovannini

Ardini

Maccaferri

et al. 2019

Advanced Optical Materials

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The spontaneous interaction between noble metals and biological scaffolds enables simple and cost‐effective synthesis of nanomaterials with unique features. Here, plasmonic silver nanorings are synthesized on a ring‐like protein, i.e., a peroxiredoxin (PRX), and used to assemble large arrays of functional nanostructures. The PRX drives the seeding growth of metal silver under wet reducing conditions, yielding nanorings with outer and inner diameters down to 28 and 3 nm, respectively. The obtained hybrid nanostructures are selectively deposited onto a solid‐state 2D membrane made of graphene in order to prepare plasmonic nanopores. In particular, the interaction between the graphene and the PRX allows for the simple preparation of ordered arrays of plasmonic nanorings on a 2D‐material membrane. This fabrication process can be finalized by drilling a nanometer scale pore in the middle of the ring. Fluorescence spectroscopic measurements in combination with numerical simulations demonstrate the plasmonic effects induced in the metallic nanoring cavity. The prepared nanopores represent one of the first examples of hybrid plasmonic nanopore structures integrated on a 2D‐material membrane. The diameter of the nanopore and the atomically thick substrate make this proof‐of‐concept approach particularly interesting for nanopore‐based technologies and applications such as next‐generation sequencing and single‐molecule detection.

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Purification and Characterization of Protein Nanotubes Assembled from a Single Bacterial Microcompartment Shell Subunit

Cited by 44 publications

References 49 publications

Bacterial microcompartments

Bacterial microcompartments

Biotechnological Advances in Bacterial Microcompartment Technology

Bio‐Assisted Tailored Synthesis of Plasmonic Silver Nanorings and Site‐Selective Deposition on Graphene Arrays

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