Purification and Characterization of Peroxidase From Anthracnose Disease Infected Papaya (Carica papaya L.)

Bari, Luthfunnesa; Hassan, Parvez; Absar, Nurul; Khatun, Serida; Hossain, Md. Azad

doi:10.3329/bjmb.v6i2.17643

Cited by 4 publications

(2 citation statements)

References 26 publications

(27 reference statements)

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“…Both isoperoxidases gave final yields significantly lower than those previously obtained (28 %) (Dicko et al, 2006) for the purification of cationic POX from sorghum. However, the yields are significantly better than those (2.5 %) obtained by Bari et al (2013) with the purification of a peroxidase from papaya. Nevertheless, it was lower than those obtained by Goyal and Chugh (2013) with the POX of pearl millet grains (41 %), those obtained by Mall et al (2013) with the POX from Citrus medica leaf (28.6 %) and those of apple seed POXs (39.99 %) obtained by Zia et al (2011).…”

Section: Resultsmentioning

confidence: 64%

Biochemical characterization of Burkina red radish (<i>Raphanus sativus</i>) peroxidase

Diao¹,

Dibala²,

Ayékoué³

et al. 2018

J. App. Bioscience.

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Objectives: Peroxidases (POX), isoenzymes were purified to homogeneity from bulbs of Raphanus sativus (Radish) grown in Burkina Faso and characterized for use as an alternative source of POX for biotechnological applications. Methodology and Results: The enzymes were purified using cation exchange, anion-exchange and hydrophobic interaction chromatography. Two isoperoxidases, cationic POX1 and anionic POX2, were isolated and purified 18.5 and 27.2 fold, respectively. Purified enzymes were found to be monomeric proteins with molecular masses of 70 kDa and 47 kDa for POX1 and POX2, respectively, as determined by SDS-PAGE. The effect of pH and temperature was done using guaicol as a substrate. The optimal pH of the both purified POX was 5.6 and 80 % of its activity was retained at pH values between 4.0-8.0 after incubation for 2 h, but POX2 appeared to be highly stable than POX1. POX1 and POX2 had optimum temperatures of activity at 35° and 40 °C, respectively. Activities of these isoenzymes were determined using different phenolic substrates in the presence and absence of ionic effectors. The results show that both POX activities were activated by bivalent cations such as Mg 2+ and Ca 2+ but were inhibited by K + , Na + , Zn 2+ , EDTA and reducing compounds. While Ba 2+ is an activator for POX2, it had no effect on POX1. They oxidize all the phenolic compounds used. The greatest rate was obtained with ABTS and guaiacol, for POX1 and POX2, respectively. Conclusions and application of findings: POXs oxidize a wide range of phenolic substrates and have high stability at a wide range of pH. These properties make these enzymes potential biotechnological tools. They could be use in industrial applications to produce food dyes, phenolic resins from natural phenolic compounds or in the construction of biosensors and kits for analyses and diagnostics. They could play also an important role in the oxidation of phenolic compounds in polluted water. This is interesting because it could be useful in finding solutions to the thorny problem of recalcitrant phenolic compounds that resist to conventional methods for bioremediation.

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Section: Resultsmentioning

confidence: 64%

Biochemical characterization of Burkina red radish (<i>Raphanus sativus</i>) peroxidase

Diao¹,

Dibala²,

Ayékoué³

et al. 2018

J. App. Bioscience.

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“…The activity of peroxidase is closely associated with the quality of plant-based products, particularly in relation to flavor, in both raw and processed foods. In numerous instances, peroxidase activity has been correlated with fruit ripening and enzymatic browning processes, often in conjunction with the activity of polyphenol oxidase [67,68].…”

Section: Oil Blocks Based On Post-pyrolysis Oilsmentioning

confidence: 99%

Assessment of the Environmental Impact of Solid Oil Materials Based on Pyrolysis Oil

Staroń,

Kijania-Kontak,

Dziadas

et al. 2023

Materials

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One method of managing used car tires is decomposition by thermochemical conversion methods. By conducting the process at temperatures of 450–750 °C, three fractions are obtained from tires: oil, gas, and solid. The liquid product of the pyrolysis of used car tires is pyrolysis oil, which consists of aromatic, polyaromatic, and aliphatic hydrocarbons. Unconventional building materials were obtained from tire pyrolysis oil and the environmental impact was evaluated. Blocks made from pyrolysis oil showed mechanical strength of up to about 1700 N. No heavy metals or polycyclic aromatic hydrocarbons, which were found in the crude heavy-PO fraction, were detected in the filtrates after incubation of the block obtained from the heavy-PO fraction at 240 °C. The highest inhibition of Sorghum saccharatum shoot (74.4%) and root (57.5%) growth was observed for solid materials from the medium-PO fraction obtained at 240 °C. The most favorable values of the parameters for the process of obtaining blocks based on post-PO were an annealing temperature of 180 °C, time of 20 h, and mass ratio of catalyst to catalyzed oil of 0.045.

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Yacon (Smallanthus sonchifolius) peel as a promising peroxidase source for the treatment of phenolic wastewater

Souza

Fernandes

Kempka

et al. 2022

Biocatalysis and Agricultural Biotechnology

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Purification and Characterization of Peroxidase From Anthracnose Disease Infected Papaya (Carica papaya L.)

Cited by 4 publications

References 26 publications

Biochemical characterization of Burkina red radish (<i>Raphanus sativus</i>) peroxidase

Biochemical characterization of Burkina red radish (<i>Raphanus sativus</i>) peroxidase

Assessment of the Environmental Impact of Solid Oil Materials Based on Pyrolysis Oil

Yacon (Smallanthus sonchifolius) peel as a promising peroxidase source for the treatment of phenolic wastewater

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