Purification and characterization of minor brain proteolipids: use of fast atom bombardment — mass spectrometry for peptide sequencing

Lepage, Pierre; Helynck, G.; Chu, Ji-Yu; Luu, Bang; Sorokine, Odile; Trifilieff, Élisabeth; Dorsselaer, Alain Van

doi:10.1016/s0300-9084(86)80161-4

Cited by 24 publications

(18 citation statements)

References 23 publications

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“…The high molecular weight form represents aggregated forms of PLP (Chan and Lees, 1974), and the lower molecular weight form appears to have similar sequence to the major myelin proteolipid forms (Lepage et al, 1986). The brain lysate preparation showed two bands at the 16 and 14 kD, two forms that were previously described (Lepage et al, 1986). In the extracts from immune cells 2 major bands were evident, the high and low molecular weight (16 and 50 kD), but there was no band of 25 kD.…”

Section: Western Blotting With Anti-plp Antibodiesmentioning

confidence: 64%

“…With the commercial antibody AHP261, the myelin preparation showed 4 major bands in the regions of 7, 16, 25, and 50 kD. The high molecular weight form represents aggregated forms of PLP (Chan and Lees, 1974), and the lower molecular weight form appears to have similar sequence to the major myelin proteolipid forms (Lepage et al, 1986). The brain lysate preparation showed two bands at the 16 and 14 kD, two forms that were previously described (Lepage et al, 1986).…”

Section: Western Blotting With Anti-plp Antibodiesmentioning

confidence: 69%

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Expression of autoimmune disease-related antigens by cells of the immune system

1998

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The process of thymic selection is critical for the generation of the mature T-cell repertoire, yet the nature of the self-peptides that serve this function is not known. Several studies suggest that tissue-specific auto-antigens are expressed in the thymus. We initiated this study to examine the expression of a panel of auto-antigens related to several autoimmune diseases in the thymus, peripheral lymphoid organs, and various cell lines. We looked for the expression of these antigens by reverse transcriptase-polymerase chain reaction, fluorescence-activated cell sorter (FACS) analysis, immunoblotting, and immunoprecipitation. We found that in the thymus there is evidence for the expression of a wide variety of disease-related self-antigens including myelin antigens, insulin, cardiac myosin, and retinal S antigen. By FACS analysis, several monoclonal anti-myelin basic protein antibodies were found to bind to immune cells. In Western blotting, we could find in the thymus and other lymphoid organs the expression of myelin basic protein, proteolipid protein, and cyclic nucleotide phosphodiesterase; in contrast, the staining for myelin oligodendrocyte glycoprotein, microtubule-associated Tau protein, and insulin were negative in these organs. The results of these studies confirm that there is evidence for the expression of a variety of auto-antigens in the immune system, both at the mRNA and protein levels, potentially enabling them to participate in the process of thymic education.

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Section: Western Blotting With Anti-plp Antibodiesmentioning

confidence: 64%

Section: Western Blotting With Anti-plp Antibodiesmentioning

confidence: 69%

Expression of autoimmune disease-related antigens by cells of the immune system

1998

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“…an isoform of PLP generated by alternative splic ing in exon 3 [14]. Lepage et al [15], later confirmed by Schindler el al. [16], found minor proteolipids in the mammalian CNS.…”

Section: Alternative Splicing Of Plpmentioning

confidence: 89%

Genetics of Pelizaeus-Merzbacher Disease

Hodes

Pratt

Dlouhy³

1993

Dev Neurosci

123

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Pelizaeus-Merzbacher disease (PMD) has been recognized as a clinical entity for more than a century. It has gradually become apparent that the disorder is a dysmyelination, in distinction to demyelinating conditions such as adrenoleukodystrophy. The failure to deposit myelin is due to decreased production of its chief protein, proteolipid protein (PLP). In about 30% of patients with the diagnosis of PMD there is a mutation in the coding portion of the proteolipid protein gene, PLP This gene is located at Xq22 so the disease in these families shows an X-linked pattern of inheritance. The expression of the mutant gene is generally recessive, but some mutations are expressed frequently in females. At least some patients with PMD that do not show mutations in the coding region of PLP demonstrate linkage between the disease and PLP. As additional mutations in PLP are discovered, it is becoming apparent that the nosology of PLP-associated disease is changing. PMD now comprises a spectrum of disorders with similar but not necessarily identical clinical pictures. Some of these disorders may be certain forms of X-linked paraplegia, SPG2. Finally, some diseases that look like PMD may not be X-linked.

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“…In the rat cultures a low molecular weight (approximately 14 kD) PLP immunoreactive protein was observed, following the same developmental course as the PLP protein which was not detected in mouse OL. These bands likely represent the low molecular weight species of proteolipid protein that is related to the major PLP reported by Lepage et al [17]. From these data it was not possible to determine if the 14-kD protein is absent from mouse OL or simply below the detection limits of this assay.…”

Section: Developmental Expression Of Myelin Proteins In Enriched Mousmentioning

confidence: 82%

“…Monitoring the num ber of OL in the mixed glial cultures during development revealed that the rat cultures contained approximately five-fold more OL than the mouse cultures, and this ratio remained constant throughout development [8,13], Even taking this difference into consideration, the number of developing OL in the enriched mouse cultures was still less than anticipated. This could indicate that mouse OL require a longer period of maturation in primary culture before they can be successfully subcultured [17] and/or that more of the mouse OL are in a bipotential stage (O-2A or 0 4) during the isolation protocol and upon replat ing in serum-containing media redifferentiate into astro cytes [16], This theory may get some support from the greater response of the enriched mouse cultures to treat ment with PDGF/FGF. Wolswijk et al [27J report the PDGF is a potent mitotic factor for 0-2A adul1 progenitor cells, and upon the addition of PDGF along with FGF to our cultures, a 25% increase in the number of GC-posi- 78 Ducliala/Asolra/Macklin Cell Surface Markers and Myelin Proteins in Cultured Oligodendrocytes tive cells in enriched mouse cultures was noted, whereas the comparable rat cultures only exhibited a 15% increase.…”

Section: Discussionmentioning

confidence: 99%

Expression of Cell Surface Markers and Myelin Proteins in Cultured Oligodendrocytes from Neonatal Brain of Rat and Mouse: A Comparative Study (Part 2 of 2)

Duchala¹,

Asotra²,

Macklin³

1995

Dev Neurosci

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Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD₃ appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.

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Purification and characterization of minor brain proteolipids: use of fast atom bombardment — mass spectrometry for peptide sequencing

Cited by 24 publications

References 23 publications

Expression of autoimmune disease-related antigens by cells of the immune system

Expression of autoimmune disease-related antigens by cells of the immune system

Genetics of Pelizaeus-Merzbacher Disease

Expression of Cell Surface Markers and Myelin Proteins in Cultured Oligodendrocytes from Neonatal Brain of Rat and Mouse: A Comparative Study (Part 2 of 2)

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