Purification and Characterization of Midgut α-Amylase in a Predatory Bug,Andralus spinidens

Abstract

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Alpha-amylase activity in the salivary glands and the midgut of Apodiphus Amygdali Germar (Hemiptera: Pentatomidae)

“…Taken collectively, it can be concluded that Ca 2 had critical role in amylolytic activity of A. spinidens salivary glands and midgut. Similar results have been observed in case of E. integriceps and A. spinidens (9,12,13).…”

“…Mehrabadi et al (11) determined amylolytic activity in the salivary glands and the midgut of Eurygaster integriceps Puton (Hemiptera: Scutelleridae). Zibaee et al (9) and Sorkhabi-Abdolmaleki et al (12) reported amylolytic activity in saliva and midgut of Andrallus spinidens Fabricius (Hemiptera: Pentatomidae). Enzymes showed various reaction toward substrates and inhibitors when they are exposed in various pH and temperature.…”

“…Zeng and Cohen (15) reported optimal pH of 6 for α-amylase of Lygus herperus and L. lineolaris. Zibaee et al (9) and Sorkhabi-Abdolmaleki et al (12) found optimal pHs of 8 and 7 for salivary and midgut α-amylase of A. spinidens. In case of temperature, obtained values are similar to finding on other hemipterans like E. integriceps, Lygus spp and A. spinidens (9)(10)(11)(12)(13)(14)(15).…”

“…Zibaee et al (9) and Sorkhabi-Abdolmaleki et al (12) found optimal pHs of 8 and 7 for salivary and midgut α-amylase of A. spinidens. In case of temperature, obtained values are similar to finding on other hemipterans like E. integriceps, Lygus spp and A. spinidens (9)(10)(11)(12)(13)(14)(15). In agro-ecosystems, many fertilizers are used to improve quality and quantity of agricultural products.…”

Biochemical characterization and overexpression of an α‐amylase (BmAmy) in silkworm, Bombyx mori

“…Alpha-amylases (α-amylases) are major digestive enzymes that hydrolyse α-1,4 glycosidic linkages at random locations of food maltopolysaccharide during digestion in animals (Da Lage, 2018). Insect α-amylases have three domains, namely, domains A, B and C. Domain A is the central domain, which comprises the core of the molecule and the catalytic amino acid residues, while domains B and C are located on each side of domain A (Sorkhabi-Abdolmaleki et al, 2014;Strobl Hao Yan, Feng Wen and Haiying Xiang contributed equally to this work. et al, 1998).…”