Purification and characterization of metallothionein-like cadmium binding protein from Asian periwinkle Littorina brevicula

Freezing and anoxia stresses induce expression of metallothionein in the foot muscle and hepatopancreas of the marine gastropodLittorina littorea

“…Invertebrate MTs also display a broader range of molecular mass, but with an unusually long N-terminal sequence, Ll-MT is still 25-38 residues longer than nearly all other invertebrate MTs reported to date. A recent study by Park et al (2002), however, described a MT containing 103 amino acids in the Asian periwinkle Littorina brevicula, so a MT sequence of ~100 amino acids may be characteristic of this group of gastropods. Unfortunately, the study by Park et al (2002) presented only a short C-terminal sequence (21 residues) covering the highly conserved molluscspecific motif (boxed region in Fig.·2A), so no comparison of the two species can be made with respect to the novel N terminus that was found in Ll-Mt.…”

“…A recent study by Park et al (2002), however, described a MT containing 103 amino acids in the Asian periwinkle Littorina brevicula, so a MT sequence of ~100 amino acids may be characteristic of this group of gastropods. Unfortunately, the study by Park et al (2002) presented only a short C-terminal sequence (21 residues) covering the highly conserved molluscspecific motif (boxed region in Fig.·2A), so no comparison of the two species can be made with respect to the novel N terminus that was found in Ll-Mt. One unusual feature of MT in both littorine species, however, was the presence of an aromatic phenylalanine residue that is not found in other molluscs.…”

Cadmium specific proteomic responses of a highly resistantPseudomonas aeruginosasan ai

“…Eluates from gel size exclusion chromatography were measured directly, without prior digestion of the samples. 25 The amount of cadmium removed by biosorbent at time t, was calculated according to:…”

Regulation of metallothionein genes in the American oyster (Crassostrea virginica): Ontogeny and differential expression in response to different stressors

“…Therefore, much of the identification and characterization of molluscan MTs has focused on the metal-inducibility, particularly by cadmium, of these proteins (Dallinger et al, 1993;Berger et al, 1995;Mackay et al, 1993;Roesijadi et al, 1989;Park et al, 2002;Khoo and Patel, 1999;Engelken and Hildebrandt, 1999) and very little is known regarding the general function and regulation of these genes. To better understand the functional role of molluscan MTs, a precise understanding of their expression patterns, the factors influencing expression, the molecular mechanisms regulating their expression, and their roles in cellular processes must be elucidated.…”