Purification and Characterization of Lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate

Abstract

The present study aimed to Purification and characterization of lipase produced by the isolate Pseudomonas aeruginosa. The results showed that crude lipase was purified by using the precipitation step of several concentrations ratios of cold acetone. The concentration ratio 4:1 gave the best specific activity 6 U/mg of protein. The subsequent purification step using gel filtration was showed that specific activity reached to 40.5U/mg of protein. The gel electrophoresis (SDS-PAGE) of lipase purity were determined. the result showed there is an appearance of a single protein band predominant in all purification steps, While The molecular weight of purified lipase was 68KDa