Purification and characterization of lignin peroxidase from white-rot fungi &lt;i&gt;Pleurotus pulmonarius &lt;/i&gt;CPG6&lt;i&gt; &lt;/i&gt;and its application in decolorization of synthetic textile dyes

Giáp, Vũ Đình; Duc, Hoang Thanh; Huong, Pham Thi; Hanh, Do Thi; Nghị, Đỗ Hữu; Duy, Vu Dinh; Quynh, Dang Thu

doi:10.2323/jgam.2022.05.005

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…These results were similar to those [2] in the case that Ca 2+ ions increased LiP activity and Hg 2+ , K + , Fe 2+ , and Mg 2+ ions decreased LiP activity, while Na + and Co 2+ ions in this study increased LiP activity. These results were also in line with [18] in case of K + , Na + , Hg 2+ , Mn 2+ , and Zn 2+ ions, but opposite to the effect of Fe 2+ , EDTA, Cu 2+ , and Mg 2+ [18]. These results were presumably because the LiP isoenzyme produced in this experiment was different from the LiP isoenzyme.…”

Section: Effect Of Metal Ions and Inhibitors On Lip From P Chrysospor...supporting

confidence: 83%

“…The LiP enzyme activity after incubation for 20, 24, and 30 h at the highest temperature of 58 °C still displayed the activity of 58, 53, and 42%, respectively, indicating that LiP from P. chrysosporium isolate ITB was a moderate thermostable enzyme. The results were better than the LiP enzyme from P. chrysosporium BKMF-1767, which had an activity of only 38% after 24 h of incubation at 60 °C [2], and much better than the purified LiP from P. pulmonarius, where the activity became 25 and 20% after 2 h incubation at 40 and 60 °C [18].…”

Section: Thermal Stability Of Lip From P Chrysosporium Itb Isolatementioning

confidence: 79%

“…Unfortunately, there was no similar research that can be used as a comparison. Recent studies have used the filtration method at the stage of concentration of crude LiP extract [18]. The use of this method minimized the occurrence of denaturation due to changes in the dielectric constant of the medium as happened in this study.…”

Section: Fractionation Profile Of Crude Extract Of Lip Enzyme From P ...mentioning

confidence: 94%

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Characterization of Lignin Peroxidase from the Suspected Novel Strain <i>Phanerochaete chrysosporium</i> ITB Isolate

Susanti

Ardyati²,

Suharjono³

et al. 2022

Indones. J. Chem.

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This study was aimed to characterize lignin peroxidase (LiP) obtained from Phanerochaete chrysosporium ITB isolate. The characterizations included molecular weight, the pH and optimum working temperature of the crude extract of the enzyme, the temperature stability, the effect of metal ions and inhibitors, their precipitation with ethanol, and the storage stability. The LiP of P. chrysosporium ITB isolates was 34 kDa. The crude extract of LiP displays high activity at pH between 3 until 5 and 26–32 °C, has good thermal stability at 26–32 °C for 20 h. The activity is affected by Pb2+, K+, Co2+, Fe2+, Cd2+, Mg2+, and Cu2+ EDTA, Na+, Cr3+, Hg2+, NaN3, Ni2+, and Ca2+ ions, is not affected by Mn2+ and Zn2+ ions, precipitated with the optimum ethanol at 64% ethanol saturation which results in an increase in specific activity of 2.3 times. The crude extract storage at 0 °C is more stable than the precipitate resulting from ethanol precipitation and resuspension from ethanol precipitation. These results strengthen that LiP from P. chrysosporium is another LiP isoenzyme that can be used for bioremediation processes. Unfortunately, the concentration using the ethanol precipitation method has not been effective, so further studies using other methods should be required.

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Section: Effect Of Metal Ions and Inhibitors On Lip From P Chrysospor...supporting

confidence: 83%

Section: Thermal Stability Of Lip From P Chrysosporium Itb Isolatementioning

confidence: 79%

Section: Fractionation Profile Of Crude Extract Of Lip Enzyme From P ...mentioning

confidence: 94%

See 1 more Smart Citation

Characterization of Lignin Peroxidase from the Suspected Novel Strain <i>Phanerochaete chrysosporium</i> ITB Isolate

Susanti

Ardyati²,

Suharjono³

et al. 2022

Indones. J. Chem.

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“…The Lignin Peroxidase (LiP) enzyme activity was assessed by Giap et al using veratraldehyde substrate at pH 3.0 (0.25 M sodium tartrate) and 10 mm H2O2. LiP from an isolated strain of Pleurotus pulmonarius CPG6 was purified with a specific activity of 6.59 U/mg by Giap et al in their research [20].…”

Section: Resultsmentioning

confidence: 99%

The Effect of 1-hydroxybenzotriazole on the Decolorization of Anthraquinone and Diazo Dyes Using Peroxidase Enzyme

ÇELEBİ,

ALTIKATOĞLU

2023

Open Journal of Nano

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Textile dyeing wastewater involving resistant dyestuffs contaminate rivers because of their color and the production of hazardous or carcinogenic intermediates, which must be removed before being released into streams. The ligninolytic enzymes have many uses, including the elimination of dyestuffs from industrial wastewater. In this study, the Lignin Peroxidase enzyme was used to eliminate synthetic solutions of Naphthol Blue Black (NBB) and Remazol Brilliant Blue R (RBBR) dyestuffs at pH 5.0 and 30 °C. In addition, the effect of 1-hydroxybenzotriazole (HOBt), a redox mediator, on dye removal at different concentrations was investigated. Once a redox mediator was added to the LiP enzyme, the percentage of RBBR dye eliminated increased from 10% to 26% after 24 hours. On the first day after adding HOBt to the LiP enzyme, the percentage of NBB dye eliminated increased from 5% to 89%. The effect of HOBt was significantly greater in the NBB than in the RBBR. The redox mediator-added Lignin Peroxidase enzyme decolorized the NBB dye more efficiently than the RBBR dye.

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A consolidated review of commercial-scale high-value products from lignocellulosic biomass

Zheng

Chen

et al. 2022

Front. Microbiol.

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For decades, lignocellulosic biomass has been introduced to the public as the most important raw material for the environmentally and economically sustainable production of high-valued bioproducts by microorganisms. However, due to the strong recalcitrant structure, the lignocellulosic materials have major limitations to obtain fermentable sugars for transformation into value-added products, e.g., bioethanol, biobutanol, biohydrogen, etc. In this review, we analyzed the recent trends in bioenergy production from pretreated lignocellulose, with special attention to the new strategies for overcoming pretreatment barriers. In addition, persistent challenges in developing for low-cost advanced processing technologies are also pointed out, illustrating new approaches to addressing the global energy crisis and climate change caused by the use of fossil fuels. The insights given in this study will enable a better understanding of current processes and facilitate further development on lignocellulosic bioenergy production.

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Purification and characterization of lignin peroxidase from white-rot fungi <i>Pleurotus pulmonarius </i>CPG6<i> </i>and its application in decolorization of synthetic textile dyes

Cited by 3 publications

References 41 publications

Characterization of Lignin Peroxidase from the Suspected Novel Strain <i>Phanerochaete chrysosporium</i> ITB Isolate

Characterization of Lignin Peroxidase from the Suspected Novel Strain <i>Phanerochaete chrysosporium</i> ITB Isolate

The Effect of 1-hydroxybenzotriazole on the Decolorization of Anthraquinone and Diazo Dyes Using Peroxidase Enzyme

A consolidated review of commercial-scale high-value products from lignocellulosic biomass

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