Purification and characterization of leukotriene A4 epoxide hydrolase from dog lung

Leukotriene A4 Metabolites Are Endogenous Ligands for the Ah Receptor

“…Additional LTA 4 metabolites are produced through hydrolysis of the strained oxirane ring, a reaction capable of proceeding through both enzyme-catalyzed and nonenzymatic reaction mechanisms, ultimately resulting in the formation of various dihydroxy products. For example, LTA 4 hydrolase catalyzes the formation of leukotriene B 4 (LTB 4 ) , , while the generation of 5( S ),6( R )-DiHETE is mediated by a soluble epoxide hydrolase , . Although the enzyme-catalyzed reactions are stereospecific, producing only a single reaction product, the nonenzymatic addition of water to LTA 4 results in the formation of multiple dihydroxy positional and stereoisomers.…”