Purification and Characterization of Highly Branched α-Glucan–Producing Enzymes from<i>Paenibacillus</i>sp. PP710

Tsusaki, Keiji; Watanabe, Hikaru; Yamamoto, Tameyoshi; Nishimoto, Tetsuya; Chaen, Hiroto; Fukuda, Shigeharu

doi:10.1271/bbb.110855

Cited by 32 publications

(12 citation statements)

References 38 publications

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“…Hence, in the present study, the true content of dietary fibre in these α-glucans has been shown to be lower. For example, although the content of dietary fibre in IMD is approximately 80 % (32) as per the AOAC 2001.03 method (27) , in the present work the true content of dietary fibre in IMD after digestion was found to be 20 % in vivo (ileorectostomised rats) and 30 % in vitro (α-amylase and BBMV). Since digestion-resistant α-glucans were found to be slowly digested, more α-glucan may potentially be available for digestion in the small intestine, perhaps the ileum and, as a result, more glucose may be potentially released.…”

Section: Discussionmentioning

confidence: 45%

Oral intake of slowly digestible α-glucan, isomaltodextrin, stimulates glucagon-like peptide-1 secretion in the small intestine of rats

et al. 2019

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To investigate whether oral intake of highly branched α-glucan isomaltodextrin (IMD) could stimulate ileal glucagon-like peptide-1 (GLP-1) secretion, we examined (1) the digestibility of IMD, (2) the digestion and absorption rates of IMD, in rat small intestine and (3) portal GLP-1 concentration in rats given IMD. In Expt 1, ileorectostomised rats were given a 3 % IMD diet for 10 d. Separately, a 16-h in vitro digestion of IMD, using porcine pancreatic α-amylase and brush-border membrane vesicles from rat small intestine, was conducted. In Expt 2, upon 24-h fasting, rats were given any of glucose, IMD and high-amylose maize starch (HAMS) (1 g/kg of body weight). In Expt 3, caecectomised rats were given 0·2 % neomycin sulphate and a 5 % IMD diet for 10 d. The in vivo and in vitro digestibility of IMD was 70–80 %. The fraction of IMD digested in vitro for the first 120 min was 67 % of that in maize starch. The AUC for 0–120 min of plasma glucose concentration was significantly lower in HAMS group and tended to be lower in IMD group than in the glucose group. Finally, we also observed that, when compared with control rats, glucose of IMD significantly stimulated and improved the concentration of portal active GLP-1 in antibiotic-administered, caecectomised rats. We concluded that IMD was slowly digested and the resulting glucose stimulated GLP-1 secretion in rat small intestine. Oral delivery of slowly released IMD glucose to the small intestine probably exerts important, yet unknown, physiological effects on the recipient.

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Section: Discussionmentioning

confidence: 45%

Oral intake of slowly digestible α-glucan, isomaltodextrin, stimulates glucagon-like peptide-1 secretion in the small intestine of rats

et al. 2019

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“…The unique structural combination of these linkages are responsible for the non-digestible nature of IMD. While these bonds are found in resistant dextrin, resistant maltodextrin and polydextrose, they are not primarily thought to be the reason for their resistance to digestion (Tsusaki et al, 2012). The resistance to digestion by resistant dextrin, resistant maltodextrin and polydextrose is thought to be the result of the various beta bonds in these molecules.…”

Section: Discussionmentioning

confidence: 99%

“…Isomaltodextrin (IMD) is a highly branched α-glucan produced enzymatically from starch using commercially available thermostable α-amylase and α-amylase, and α-glucosyltransferase and α-amylase derived from Paenibacillus alginolyticus PP710 (Tsusaki et al, 2012). IMD is a white powder with no odor, it is freely soluble in water and dissolves into a clear solution, and therefore is unlikely to alter the smell, color or taste of a product in which it is used.…”

Section: Introductionmentioning

confidence: 99%

Safety assessment of a soluble dietary fiber, isomaltodextrin, enzymatically produced from starch

Sadakiyo

Inoue

Ishida

et al. 2017

Fundam. Toxicol. Sci.

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-A series of safety studies were conducted using isomaltodextrin (IMD), a new dietary fiber that is produced from starch using enzymes. IMD consists of only alpha linkaged glucose molecules, has an average molecular weight of approximately 5,000, is freely soluble in water, and contains greater than 80% fiber (AOAC 2001.03). No genotoxicity was observed when IMD was assayed in standardized bacterial reverse mutation, micronucleus, and chromosome aberration tests. The LD 50 of IMD was found to be more than 2,000 mg/kg in an acute toxicity study in rats, and the no observed adverse effect level (NOAEL) was determined to be 1,000 mg/kg/day in a 90-day subacute gavage toxicity study in rats. No animals died, and no abnormal findings due to consumption of IMD were observed in either of these studies. Both NOAEL values were the highest doses tested. The NOAEL for loose stools was examined in humans in two separate studies. Based on these results, the NOAEL for IMD-related loose stools was considered to be 0.8 g/kg-BW. In a 4-week high-dose ingestion study in humans and a 12-week low-dose ingestion study in humans, laboratory values were found to be within the normal range of variation. The results of the current safety assessment studies suggest that isomaltodextrin is safe for human consumption.

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“…Isomaltodextrin (IMD) is a highly branched α-glucan produced enzymatically from starch (Tsusaki et al, 2012). IMD contains only glucose units and alpha bonds, which consist of the following glucosidic linkages: 17% α-1 (nonreducing end groups), 3% α-1,3, 19% α-1,4, 49% α-1,6, 7% α-1,3,6, and 5% α-1,4,6 (Tsusaki et al, 2012). The average molecular weight of IMD is approximately 5,000 ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Safety assessment of isomaltodextrin-producing enzymes from <i>Paenibacillus alginolyticus</i>

Sadakiyo

Watanabe

Mitsuzumi

et al. 2019

Fundam. Toxicol. Sci.

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Isomaltodextrin-producing enzymes (PP enzyme) include two extracellular enzymes (α-glucosyltransferase and α-amylase) produced by Paenibacillus alginolyticus PP710. These enzymes are essential for producing isomaltodextrin (IMD), a highly branched α-glucan, from starch. In this study, we evaluated the safety of this PP enzyme. No genotoxicity was observed when the PP enzyme was assayed in standardized bacterial reverse mutation and chromosome aberration tests. An acute toxicity study in rats showed no toxic effects of PP enzyme at 2,000 mg/kg. No animals died, and no effects of enzyme administration were observed in a 14-day repeated oral-dose toxicity study in rats at the maximum dose of 1,000 mg/kg/day. The no observed adverse effect level was determined to be 1,000 mg/kg/day in a 90-day subchronic gavage toxicity study in rats. No animals died, and no abnormal findings due to consumption of the PP enzyme were observed in this study. These safety evaluation results demonstrated that the PP enzyme was safe for application as an ingredient in the manufacturing of the food ingredient IMD.

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Purification and Characterization of Highly Branched α-Glucan–Producing Enzymes fromPaenibacillussp. PP710

Cited by 32 publications

References 38 publications

Oral intake of slowly digestible α-glucan, isomaltodextrin, stimulates glucagon-like peptide-1 secretion in the small intestine of rats

Oral intake of slowly digestible α-glucan, isomaltodextrin, stimulates glucagon-like peptide-1 secretion in the small intestine of rats

Safety assessment of a soluble dietary fiber, isomaltodextrin, enzymatically produced from starch

Safety assessment of isomaltodextrin-producing enzymes from <i>Paenibacillus alginolyticus</i>

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