Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: A potential additive for laundry detergents

Jain, Deepti; Pancha, Imran; Mishra, Sanjiv K.; Shrivastav, Anupama; Mishra, Sandhya

doi:10.1016/j.biortech.2011.10.081

Cited by 107 publications

(82 citation statements)

References 33 publications

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“…While at 7.5% NaCl there was slight decrease in the protease production to about 79 % of the maximal yield, drastic decrease in enzyme production occurred at 10% NaCl to about 20% of the maximal protease production (Figure 7). The ability of strain AK-R to grow over a wide range of NaCl concentrations (up to 25%), with maximum cells growth at 2.5-5% NaCl, indicated the halo-tolerance nature of this organism (JAIN et al, 2012). Studies on the effect of salinity on growth of halotolerant bacteria have shown a change in the polar lipid composition of the cell membranes and an increased salt concentration creates change in the lipid resulting in decrease of growth rate causing reduced enzyme production (TANG, X. M. et al, 2004).…”

Section: Effect Of Salinitymentioning

confidence: 98%

“…However, other organic nitrogen sources were found to support protease production in other microorganisms including yeast extract (DENG et al, 2010); beef extract (KUMAR et al, 2014), casamino acids (JAIN et al, 2012), skim milk (GOUDA, 2006), peptone (OSKOUIE et al, 2008), and others (SRIVIDYA; MALA, 2011). Slight decrease of alkaline protease production by strain AK-R at high gelatin concentration may due to repression role of excessive amino acid and ammonium ions in alkaline protease production (JOO et al, 2003;CHU, 2007).…”

Section: Effect Of Nitrogen Sourcementioning

confidence: 99%

“…Within the enzyme market, proteases alone contribute approximately 60% of the total sales in the world; and microbial proteases constitute approximately 40% of the total worldwide production of enzymes (JAIN et al, 2012;. Alkaline proteases, characterized by high activity and stability in high alkaline range, have various industrial applications including leather, pharmaceuticals, foods, diagnostic reagents, soy processing, peptide synthesis, re-cycling of X-ray film for silver extraction, and bioconversion of chitinous materials (SHAH et al, 2010;JELLOULI et al, 2011;JOSHI et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…However, the main application of alkaline proteases is in detergent industry, accounting for approximately 30% of the total world enzyme production, which is due to the alkaline pH of laundry detergents ingredients (HADDAR et al, 2009a). Alkaline proteases are used in detergents formulations, with other hydrolytic enzymes, as cleaning additives to facilitate the breakdown and release of proteins (JAIN et al, 2012). The increased commercial demand for highly active alkaline proteases with high specificity and stability continues to stimulate the search for new enzymes (VIJAYARAGHAVAN; VINCENT, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

Ibrahim

Al‐Salamah

Elbadawi³

et al. 2016

Biosci. J.

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Alkaline proteases are hydrolytic enzymes that cleave peptide bonds in proteins and peptides in alkaline conditions, which occupy a pivotal importance with respect to their industrial applications. This study aimed to isolate new alkaline protease producing alkaliphilic bacteria from Egyptian soda lakes and optimize the fermentation process to enhance the enzyme production. The extensive screening process of the samples collected from Egyptian soda lakes resulted in isolation of a potent alkaline protease producing alkaliphilic strain AK-R. The isolate was identified as Bacillus agaradhaerens strain AK-R based on 16S rRNA gene analysis (99%). Wheat bran and gelatin supported maximum alkaline protease production as carbon and nitrogen sources, respectively. Strain AK-R is halo-tolerant thermotolerant alkaliphilic bacterium in nature, as it can grow over a wide range of NaCl concentrations (up to 25%) and up to 55 °C, with maximal growth and enzyme production at 2.5-5%, and pH 11 at 35 °C. Among the tested cations, only Mg 2+ and Ca 2+ ions significantly enhanced the enzyme production by about 1.2, and 1.3 fold compared to control, respectively. Alkaline protease secretion was coherent with the growth pattern, reaching maximal yield after about 32 h (mid stationary phase). In conclusion a new halo-tolerant thermo-tolerant alkaliphilic alkaline protease producing Bacillus agaradhaerens strain AK-R was isolated from Egyptian soda lakes. Optimization of the nutritional and cultivation conditions resulted in increase of enzyme yield by 20 fold. Strain AK-R and its extracellular alkaline protease with salt, pH and temperature, tolerance signify their potential application in laundry and pharmaceuticals industries.

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Section: Effect Of Salinitymentioning

confidence: 98%

Section: Effect Of Nitrogen Sourcementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

Ibrahim

Al‐Salamah

Elbadawi³

et al. 2016

Biosci. J.

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“…Serine proteases find industrial importance [6]. Thermal stability and activity at alkaline pH ensures applications of alkaline protease in detergent formulation [7]. Studies have revealed that bacteria capable of producing alkaline proteases have keratinolytic activity [8].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Puntambekar

Dake

2017

Asian J Pharm Clin Res

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Objective: The main objective of this study is to investigate the industrial applications of a thermophillic alkaline protease from a hot water spring bacterial isolate "A" and to study its production, optimization, and purification. Methods:The alkaline protease was produced using shake flask studies maintaining a pH of 9.0 and a temperature of 50°C. Optimization studies of the enzyme were carried out using variable pH, temperature, organic carbon, and nitrogen sources followed by purification of the enzyme using DEAE-cellulose ion exchange chromatography technique. Stability of the enzyme was analyzed in the presence of organic solvents and surfactants. The efficiency of the enzyme in the removal of proteinaceous stains in the presence of strong detergents under extreme conditions was assessed. The fibrinolytic activity of the enzyme in dissolving the blood clot was confirmed. Results:The isolated alkaline protease was purified to homogeneity with a 16-fold increase. Media optimization studies revealed that 1% glucose and 1 % casein-induced the production of alkaline protease. The purified enzyme retained stability in the presence of ethanol, methanol, and acetone and surfactants such as 0.5% (w/v) sodium dodecyl sulfate (SDS) and 0.5% (v/v) Triton-X-100. The isolated alkaline protease successfully removed the proteinaceous stains and showed significant results in the dissolution of blood clot. Conclusion:The above experimental results confirm that the isolated enzyme has both thermophilic and alkaliphilic protease properties. Thereby the enzyme finds promising industrial applications even in extreme conditions.

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Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and α-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity (ࣙ7.5 IU).

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Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: A potential additive for laundry detergents

Cited by 107 publications

References 33 publications

Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain ak-r isolated from egyptian soda lakes

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

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