Purification and characterization of glutathione reductase from rainbow trout (Oncorhynchus mykiss) liver and inhibition effects of metal ions on enzyme activity

Tekman, Bilge; Özdemir, Haşan; Şentürk, Murat; Çiftçi, Mehmet

doi:10.1016/j.cbpc.2008.04.005

Cited by 48 publications

(46 citation statements)

References 30 publications

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“…Thus, we can infer that in P. lineatus the gill GPx is acting with greater intensity in the elimination of reactive species than the CAT. The GR has an important role as an antioxidant within the cell, since it is responsible for catalysing the reduction of GSSG to its reduced form, GSH, necessary for cell protection against oxidative stress (Tekman et al, 2008). Fish from polluted sites can show increased GR activity because of the high concentration of peroxidative components present in aquatic environments (Stephensen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L -1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione Stransferase (GST). The antioxidant defense proved to be greatly affected by MA exposure leading to changes, both in gills and liver, in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and in the content of tripeptide glutathione (GSH). Lipid peroxidation was not detected, but damage to DNA molecule was observed in blood cells. In conclusion, it can be state the lyophilized MA is able to promote changes in the biochemical and genetic parameters of P. lineatus.As microcistinas são metabólitos secundários produzidos por diferentes espécies de cianobactérias, como a Microcystis aeruginosa (MA). Neste estudo, os efeitos bioquímicos e genéticos de liofilizado de MA foram avaliados para juvenis da espécie de peixe Neotropical Prochilodus lineatus expostos a 1 ou 2 mg L -1 de liofilizado de MA (grupo tratado) ou apenas à água (grupo controle), em testes de toxicidade estáticos, durante 24 e 96 h. As brânquias e o fígado foram usados para as análises das enzimas de biotransformação e defesas antioxidantes, células do sangue e das brânquias para análises genéticas e no cérebro e músculo foi determinada a atividade da acetilcolinesterase (AChE). Os resultados mostraram ativação da via de biotransformação devido ao aumento da atividade da CYP1A hepática e da atividade da glutationa S-transferase (GST) hepática e branquial. As defesas antioxidantes foram muito afetadas pela exposição a MA levando a alterações, tanto no fígado como nas brânquias, na atividade da superóxido dismutase (SOD), da catalase (CAT), da glutationa peroxidase (GPx), glutationa redutase (GR) e no conteúdo do tripeptídeo glutationa (GSH). Apesar dessas alterações a peroxidação lipídica não foi detectada em nenhum dos tecidos, mas danos na molécula de DNA foram observados nas células do sangue. Em conclusão, pode-se afirmar que o liofilizado de MA é capaz de promover alterações em parâmetros bioquímicos e genéticos de P. lineatus.

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Section: Discussionmentioning

confidence: 99%

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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“…For instance, medical drugs (Alici et al, 2008;Ekinci et al, 2007a ), metal ions (Ekinci et al, 2007b;Tekman et al, 2008), pesticides and fungicides Ceyhun et al, 2010a) generally inhibit the enzymes at very low concentrations (Ekinci & Beydemir, 2010a). These inhibitions could be very dangerous in some cases (Bayram et al, 2008;Senturk et al, 2009a,b;Coban et al, 2009;Alp et al, 2010).…”

Section: Modulation Of the Enzyme Activitymentioning

confidence: 99%

Interactions of Fungicides and Pesticides with Specific Enzymes

Ekinci¹,

Şentürk²

2010

Fungicides

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“…These enzymes are cooperative in several aspects 32,33 . Our groups recently investigated the interaction of human erythrocyte GR enzyme with several types of analgesic, anaesthetic drugs and antibiotics as well as metal ions 5,[9][10][11][12][13][14][15] .…”

Section: Resultsmentioning

confidence: 99%

“…Some studies found either increases or decreases in mammalian enzyme activities, and the inhibitor or activator effects of some antibiotics have been rarely investigated. Nevertheless, a few recent investigations regarding the inhibitory actions of different type of drugs and chemicals on GR have been reported by our group [9][10][11][12][13][14][15] . Cefuroxime is a second-generation cephalosporin antibiotic whereas ceftriaxone, ceftizoxime and cefoperazone are third-generation cephalosporin antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of cord blood antioxidant enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase to different antibiotics:in vitroapproach

Ekinci

Çankaya

Gül

et al. 2012

Journal of Enzyme Inhibition and Medicinal Chemistry

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Purification and characterization of glutathione reductase from rainbow trout (Oncorhynchus mykiss) liver and inhibition effects of metal ions on enzyme activity

Cited by 48 publications

References 30 publications

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Interactions of Fungicides and Pesticides with Specific Enzymes

Susceptibility of cord blood antioxidant enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase to different antibiotics:in vitroapproach

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