Purification and characterization of glutamate decarboxylase from Neurospora crassa conidia.

Hao, R; Schmit, Joseph C.

doi:10.1016/s0021-9258(19)67765-3

Cited by 39 publications

(2 citation statements)

References 27 publications

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“…The values obtained for these two parameters were 1.83 × 10 4 µmol min -1 mg -1 of protein and 6.25 mmol L -1 , respectively. When compared with other published K m values (21)(22)(23)(24)(25)(26) and not considering that this was evaluated under flow conditions, in this work, it could be concluded that only Osuji (24) reported a higher value (7.7 mmol L -1 ) for the enzyme extracted from Arachis hypogaea (peanuts). This result indicates lower affinity of the enzyme for the considered substrate.…”

Section: Resultsmentioning

confidence: 46%

Simple and Inexpensive Flow l-Glutamate Determination Using Pumpkin Tissue

Arruda¹,

Filho²,

Montenegro³

et al. 2003

J. Agric. Food Chem.

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This work refers to a very easy to implementate flow injection system with potentiometric detection for l-glutamate determination in food samples. The proposed procedure is based on measurement of carbon dioxide produced by decarboxylation of l-glutamate catalyzed by l-glutamate decarboxylase (E.C. 4.1.1.1.5) from Cucurbita maxima (pumpkin). The FI potentiometric system includes an enzymatic reactor with a length of 8 cm and thickness of 5 mm packed with 200 mg of a C. maxima outer layer cut in to small pieces. The proposed procedure allowed l-glutamate determinations in the concentration interval of 10-100 mmol L(-1) for an injected sample volume of 50 microL. A phosphate buffer (0.1 mol L(-1), pH 5.5) solution flowing at 1.4 mL min(-1) was used as the carrier solution in the system. The results obtained in the analysis of food samples revealed a relative error lower than 5% when compared with those provided by the spectrophometric reference procedure. The immobilized reactor retained its initial activity for 21 days. It was possible to measure 40 samples/h with the flow system proposed.

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Section: Resultsmentioning

confidence: 46%

Simple and Inexpensive Flow l-Glutamate Determination Using Pumpkin Tissue

Arruda¹,

Filho²,

Montenegro³

et al. 2003

J. Agric. Food Chem.

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“…[66,68] GAD is an enzyme that catalysers the decarboxylation of glutamate to GABA and CO 2 . [66,69] GABA is the major inhibitory neurotransmitter found in the CNS that is involved in learning and memory as well as the mechanisms underlying aggressive behaviour in animals. [70] GABAergic system closely interacts with the dopaminergic, serotonergic and cholinergic neurotransmitter systems.…”

Section: Effect Of Ahm and Ethanol On Cortical Expressions Of Nf-κb A...mentioning

confidence: 99%

Aggressive‐like behaviour and neurocognitive impairment in alcohol herbal mixture‐fed mice are associated with increased neuroinflammation and neuronal apoptosis in the prefrontal cortex

Ajayi

Melete

Ben‐Azu

et al. 2022

J Biochem & Molecular Tox

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Alcohol-induced aggression and related violence is a serious and common social problem globally. Alcohol use is increasingly found in the form of alcoholic herbal mixtures (AHM) with indiscriminate and unregulated alcohol content. This study investigated the effects of AHM on aggressive-like, neurocognitive impairment and brain biochemical alteration in mice. Thirty-two male resident mice were paired housed with female mice for 21 days in four groups (n = 8). Resident mice were treated orally with normal saline, AHM, ethanol and AHM + ethanol daily for 14 days. Aggressive-like behaviour was scored based on the latency and frequency of attacks by the resident mouse on the intruder. Neurocognitive impairment was determined using the Y-maze test (YMT) and novel object recognition test (NORT).Acetylcholinesterase, glutamic acid decarboxylase (GAD), pro-inflammatory and oxidative stress parameters were determined in the prefrontal cortex (PFC).Neuronal morphology, cytochrome c (Cyt-c) and nuclear factor-kappa B (NF-ĸB) expressions were determined. AHM and in combination with ethanol showed an increased index of aggression typified by frequency of attack and reduced latency to attack when compared to normal saline-treated animals. Co-administration of AHM and ethanol significantly reduced cognitive correct alternation (%) and discrimination index in the YMT and NORT, respectively. AHM and ethanol increased acetylcholinesterase, Pro-inflammatory cytokines and oxidative stress parameters while they reduced GAD. There were significantly reduced neuronal counts and increased expression of Cyt-c and NF-ĸB, respectively Alcoholic herbal mixture increased aggressiveness and caused neurocognitive impairment via increased oxidoinflammatory stress in the prefrontal cortex.

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Enzyme Assays

Bisswanger¹

2011

Practical Enzymology

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Purification and characterization of glutamate decarboxylase from Neurospora crassa conidia.

Cited by 39 publications

References 27 publications

Simple and Inexpensive Flow l-Glutamate Determination Using Pumpkin Tissue

Simple and Inexpensive Flow l-Glutamate Determination Using Pumpkin Tissue

Aggressive‐like behaviour and neurocognitive impairment in alcohol herbal mixture‐fed mice are associated with increased neuroinflammation and neuronal apoptosis in the prefrontal cortex

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