Purification and Characterization of Glucose 6‐Phosphate Dehydrogenase, 6‐Phosphogluconate Dehydrogenase, and Glutathione Reductase from Rat Heart and Inhibition Effects of Furosemide, Digoxin, and Dopamine on the Enzymes Activities

Adem, Şevki; Çiftçi, Mehmet

doi:10.1002/jbt.21793

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…The results indicated that the G6PD, 6PGD, and GR enzymes are homodimers. Similar results have been observed for rat kidney, rat lung, rat heart, trout liver, human erythrocytes, and calf liver …”

Section: Discussionsupporting

confidence: 88%

In vitro and in vivo effects of iron on the expression and activity of glucose 6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

Baltaci

Guler

Ceylan

et al. 2018

J Biochem & Molecular Tox

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Iron is an indispensable element for vital activities in almost all living organisms. It is also a cofactor for many proteins, enzymes, and other essential complex biochemical processes. Therefore, iron trafficking is firmly regulated by Hepcidin (Hamp), which is regarded as the marker for iron accumulation. The disruption of iron homeostasis leads to oxidative stress that causes various human diseases, but this mechanism is still unclear. The aim of this study is to provide a better in vivo and in vitro understanding of how long‐term iron overload affects the gene expression and activities of some antioxidant enzymes, such as glucose 6‐phosphate dehydrogenase (G6PD), 6‐phosphogluconate dehydrogenase (6PGD), and glutathione reductase (GR) in the spleen. The findings of this study show that iron overload reduces the gene expression of G6pd, 6pgd, and Gr, but its actual effect was on the protein level.

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Section: Discussionsupporting

confidence: 88%

In vitro and in vivo effects of iron on the expression and activity of glucose 6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

Baltaci

Guler

Ceylan

et al. 2018

J Biochem & Molecular Tox

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“…They found that K I values were in the range of 113.30 to 474.70 μM. Adem and Ciftci 96 purified G6PD and 6PGD enzymes from rat hearts and studied the inhibitory effects of dopamine, furosemide, and digoxin on these enzyme activities. These drugs displayed a low millimolar inhibitory effect.…”

Section: Resultsmentioning

confidence: 99%

Screening of in vitro and in silico effect of Fluorophenylthiourea compounds on glucose 6‐phosphate dehydrogenase and 6‐phosphogluconate dehydrogenase enzymes

Yıldız

Demir

Küfrevioğlu

2022

J of Molecular Recognition

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Inhibition studies of enzymes in the pentose phosphate pathway (PPP) have recently emerged as a promising technique for pharmacological intervention in several illnesses. Glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) are the most important enzymes of the PPP. For this purpose, in the current study, we examined the effect of some fluorophenylthiourea on G6PD and 6PGD enzyme activity. These compounds exhibited moderate inhibitory activity against G6PD and 6PGD with K I values ranging from 21.60 ± 8.42 to 39.70 ± 11.26 μM, and 15.82 ± 1.54 to 29.97 ± 5.72 μM, respectively.2,6-difluorophenylthiourea displayed the most potent inhibitory effect for G6PD, and 2-fluorophenylthiourea demonstrated the most substantial inhibitory effect for 6PGD. Furthermore, the molecular docking analyses of the fluorophenylthioureas, competitive inhibitors, were performed to understand the binding interactions at the enzymes' binding site.6-phosphogluconate dehydrogenase, fluorophenylthiourea, glucose 6-phosphate dehydrogenase, molecular docking | INTRODUCTIONOne of the main intracellular reducing agents, nicotinamide adenine dinucleotide phosphate (NADPH), is mainly produced by the pentose phosphate pathway (PPP) and is crucial for the thioredoxin and glutathione systems. 1-4 PPP is the main pathway for glucose catabolism and the biosynthesis of aromatic amino acids, nucleic acids, glucose 6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) enzymes. These enzymes are the most important enzymes of the PPP play an essential role in the formation of NADPH. [5][6][7][8] On the other hand, an activated PPP affects tumor metabolism, such as angiogenesis, favoring tumor metastasis, promoting malignant transformation, protecting cells from apoptosis, and increasing tumor progression. [9][10][11][12] In recent years, overexpression of PPP enzymes in many types of cancer has been the focus of interest for researchers. The mechanism of PPP enzymes associated with cancer progression is still under investigation. The research found that 6PGD was regulated in many types of cancer, such as colon, leukemia, breast, ovarian, liver, and thyroid. [13][14][15] Thiourea structures have several biological activities, like anticancer and antibacterial activities, and especially on the central nervous system of rodents, they have a high impact. [16][17][18] The thiourea skeleton has an essential role in pharmaceutical chemistry. Studies have shown solid cytotoxic activity against cancer cells. [19][20][21] The desired inhibitory activity of these drugs against various inhibitors is crucial in the death of cancer cells. Recently, evaluation of novel derivatives of thiourea as anti-angiogenic and antitumor agents has been published. [22][23][24]

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“…The impact of these drug active agents that are often given for treatment on people is gaining importance. The inhibitory effects were studied on Digoxin Glucose 6-Phosphate Dehydrogenase, 6-phosphogluconate dehydrogenase and glutathione reductase enzymes [23]. Besides these, digoxin is a drug used in health care today generally for cardiac insufficiency and atrial fibrillation disorders [24].…”

Section: Resultsmentioning

confidence: 99%

Investigation Effects of Some Heart Disorder Drugs on Human Carbonic Anhydrase I

Kaplan¹,

Kuzucu²,

Aktaş³

et al. 2017

HJBC

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K alp rahatsızlıklarının tedavisinde, kan yolu ile etki gösteren metoprolol tartarat ve digoksin ilaçları yaygın bir şekilde kullanılmaktadır. Bu ilaç etken maddelerinin kullanımının hayati öneme sahip olması, bunların kanda bulunan enzimlerin aktiviteleri üzerine inhibisyon etkilerinin araştırılması bu çalışmanın önemi ortaya koymaktadır. Bu çalışmada hedeflenen, metoprolol tartarat ve digoksin ilaç etken maddelerinin insan eritrositi karbonik anhidraz I (CA-I) izoenzimi üzerine in vitro etkilerinin araştırılmasıdır. CA-I izoenzimi Sefaroz-4B-L-tirozin-sülfamid afinite kromatografisi yöntemi ile insan eritrositlerinden saflaştırıldı. CA-I izoenzimi %62 verimle yaklaşık 115 kat saflaştırılmıştır. İnhibisyon çalışmaları sonucunda iki inhibitör de yarışmasız inhibisyon göstermiştir. K i değerleri metoprolol tartarat 5.49±1.86 mM ve digoksin için 0.0914±0.0062 mM olarak belirlenmiştir. Anahtar KelimelerKarbonik anhidraz I, afinite kromotografisi, ilaç, inhibisyon.

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Purification and Characterization of Glucose 6‐Phosphate Dehydrogenase, 6‐Phosphogluconate Dehydrogenase, and Glutathione Reductase from Rat Heart and Inhibition Effects of Furosemide, Digoxin, and Dopamine on the Enzymes Activities

Cited by 10 publications

References 34 publications

In vitro and in vivo effects of iron on the expression and activity of glucose 6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

In vitro and in vivo effects of iron on the expression and activity of glucose 6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

Screening of in vitro and in silico effect of Fluorophenylthiourea compounds on glucose 6‐phosphate dehydrogenase and 6‐phosphogluconate dehydrogenase enzymes

Investigation Effects of Some Heart Disorder Drugs on Human Carbonic Anhydrase I

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