Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea

Purification of an Intracellular Fibrinolytic Protease from Ganoderma Lucidum Vk12 and its Susceptibility to Different Enzyme Inhibitors

“…The sensitivity of the purified protease to EDTA clearly shows that it could be a metalloprotease. A similar observation had been made by some authors previously [14] with the same organism; however, the molecular mass of the enzyme was estimated to be 54 kDa, which clearly indicates that the enzyme purified in this study is different from the one reported earlier. The fibrinolytic proteases of the wild mushroom, Tricholoma saponaceum, and the cultivated Pleurotus ostreatus were sensitive to EDTA and 1, 10 phenanthroline [18].…”

Purification and Characterization of a Fibrinolytic Protease from a Culture Supernatant ofFlammulina velutipesMycelia

“…2C). The purified FVP-I was different from that determined for the 20 kDa of GfMEP from Glifora frondosa, 21) 19 kDa of PoMEP purified from Pleurotus ostreatus, 22) 21 kDa of AmMP purified from Armillaria mellea, 20) and 52 kDa of fibirnolytic enzyme purified from Cordyceps militaris. 24) As shown in Fig.…”

“…[16][17][18] These constitute an important source of thrombolytic agents. Some reports have described the fibrinolytic activity of certain edible mushrooms, including Armillaria mellea, 19,20) Grifora frondosa, 21) Pleurotus ostreatus, 22,23) and Cordyceps militaris. 24) The golden needle mushroom, Flammulina velutipes, belonging to the family Tricolomataceae (Hymenomycetes, Basidiomycota), is quite popular in Korea, Japan, China, and Taiwan.…”

Isolation and identification of a novel fibrinolytic <i>Bacillus tequilensis</i> CWD-67 from dumping soils enriched with poultry wastes

“…are 21 kDa, and 18 kDa, respectively (Mander et al, 2011;Wang et al, 2009). In contrast, the fibrinolytic enzyme isolated in this study was much less than that from Bacillus subtilis ICTF-1 (28 kDa) (Mahajan et al, 2012), while it was greater than that from A. mellea (20 kDa) (Lee et al, 2005) and (14 kDa) from P. eryngii (Cha et al, 2010).…”

“…Hence, secondary effects, such as platelet activation related plasmin formation caused by plasminogen activators, could be avoided (Wu et al, 2009). The degradation pattern of fibrin by the purified enzyme showed that the enzyme preferentially hydrolyzed the α chain of fibrin, followed by the β chain, and finally the γ-γ chain, with a pattern similar to those of the enzymes from C. militaris and Armillaria mellea (Lee et al, 2005).…”