Purification and Characterization of Extracellular Cr(VI) Reductase from Bacillus amyloliquefaciens (CSB 9) isolated from Chromite Mine Soil for Detoxification of Hexavalent Chromium
Abstract:Chromate is widely used industrial pollutant which can be detoxified effectively using chromate reductase from chromium resistant bacteria. A soluble Cr(VI) reductase from Bacillus amyloliquefaciens (CSB 9) was extracted and purified to electrophoretic homogeneity through (NH4)2SO4 precipitation followed by dialysis and gel filtration chromatography with specific activity of 0.167 U/mg and 6% yield with 30.36-fold increase in purity. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molec… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.