Purification and characterization of extracellular laccase produced by<i>Ceriporiopsis subvermispora</i>and decolorization of triphenylmethane dyes

Chmelová, Daniela; Ondrejovič, Miroslav

doi:10.1002/jobm.201600152

Cited by 29 publications

(13 citation statements)

References 51 publications

(70 reference statements)

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“…Enzymes allow the development of sustainable catalytic processes compared to their chemical counterparts, since they are more specific to target substrates, allow a lower consumption of chemicals, and are natural, renewable, and biodegradable (Datta et al, 2013). For these reasons, enzymes are important catalysts, exhibiting high potential in many industrial applications, such as in the chemical, textile, food, and pharmaceutical industries (Chapman et al, 2018;Rajhans et al, 2021). However, to make these enzymatic processes economically viable, it is crucial to achieve the reuse of the selected biocatalyst while maintaining its catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

Sustainable liquid supports for laccase immobilization and reuse: Degradation of dyes in aqueous biphasic systems

Ferreira

Valente

Castro

et al. 2021

Biotech & Bioengineering

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Novel liquid supports for enzyme immobilization and reuse based on aqueous biphasic systems (ABS) constituted by cholinium-based ionic liquids (ILs) and polymers for the degradation of dyes are here proposed. The biocatalytic reaction for dye decolorization using laccase occured in the biphasic medium, with the enzyme being "supported" in the IL-rich phase and the dye and degradation products being enriched in the polymer-rich phase. An initial screening of the laccase activity in aqueous solutions of ABS constituents, namely cholinium dihydrogen citrate ([Ch][DHC]), cholinium dihydrogen phosphate ([Ch][DHP]), cholinium acetate ([Ch][Acet]), polypropylene glycol 400 (PPG 400), polyethylene glycol 400 (PEG 400) and K 2 HPO 4 was carried out. Compared to the buffered control, a relative laccase activity of up to 170%, 257%, and 530% was observed with PEG 400, [Ch][DHP], and [Ch][DHC], respectively. These ABS constituents were then investigated for the in situ enzymatic biodegradation of the Remazol Brilliant Blue R (RBBR) dye. At the optimized conditions, the ABS constituted by PPG 400 at 46 wt% and [Ch][DHC]at 16 wt% leads to the complete degradation of the RBBR dye, further maintaining the enzyme activity. This ABS also allows an easy immobilization, recovery, and reuse of the biocatalyst for six consecutive reaction cycles, achieving a degradation yield of the dye of 96% in the last cycle. In summary, if properly designed, high enzymatic activities and reaction yields are obtained with ABS as liquid supports, while simultaneously overcoming the safety and environmental concerns of conventional organic solvents used in liquid-liquid heterogeneous reactions, thus representing more sustainable biocatalytic processes.

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Section: Introductionmentioning

confidence: 99%

Sustainable liquid supports for laccase immobilization and reuse: Degradation of dyes in aqueous biphasic systems

Ferreira

Valente

Castro

et al. 2021

Biotech & Bioengineering

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“…The most common method for the production of laccase and other lignocellulolytic enzymes is based on the cultivation of appropriate microbial producer, such as white-rot fungus, on the lignocellulosic material such as wood, straw or hay (Gonzáles et al 2013;Kneževic et al 2013;Chmelová and Ondrejovič 2016). The results of this method are not suitable for the commercial production of target enzymes.…”

Section: Resultsmentioning

confidence: 99%

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

Hazuchová¹,

Chmelová²,

Ondrejovič³

2017

Nova Biotechnologica Et Chimica

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The lignocellulolytic enzymes are routinely produced by submerged fermentation using lignocellulosic material, but for more effective production, it would be suitable to precede the production phase on the lignocellulose by propagation phase in the nutrition medium suitable for growth of the fungi. Therefore, the aim of this study was to increase the laccase production by the white-rot fungus Pleurotus ostreatus by two-step cultivation strategy. In the first step, propagation medium was optimized for the maximal biomass growth, the second step included the laccase production by produced fungal biomass in media with the selected lignocellulosic material (pine sawdust, alfalfa steam and corn straw). From our experiments, parameters such as glucose concentration, yeast extract concentration and pH of propagation medium were selected as key factors affecting growth of P. ostreatus. The optimal conditions of propagation medium for maximal fungal growth determined by response surface methodology were: glucose concentration 102.68 g/L, yeast extract concentration 43.65 g/L and pH of propagation medium 7.24. These values were experimentally verified and used statistical model of biomass production prediction was appropriate adjusted. Thus prepared fungal biomass produced in the media with lignocellulose approximately 9-16 times higher concentrations of the laccase in 3 times shorter time than the fungal biomass without propagation phase in optimized propagation medium.

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“…Previous studies have reported that prior upregulation of the required enzymes is the most efficient method for degrading dyes (Ma et al, 2016;Kittl et al, 2012). Microbial degradation of dyes primarily depends on enzymes related to lignin degradation, such as laccase, lignin peroxidase and manganese peroxidase (Grandes-Blanco et al, 2020;Chmelová and Ondrejovic, 2016;Jia et al, 2004;Wang et al, 2016). Pleurotus eryngii, a white-rot fungus, has a high potential to produce various ligninolytic enzymes capable of degrading lignin, cellulose and hemicelluloses.…”

Section: Introductionmentioning

confidence: 99%

New cost-effective mediator enhanced enzymatic degradation of aniline blue

Lu¹,

Luo²,

Dong³

et al. 2021

JEB

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Aim: In this study, the effects of various agricultural wastes as low-cost, readily available alternatives to chemical mediators for the enzymatic degradation of aniline blue were investigated. Methodology: Enzymes for degrading aniline blue were obtained through solid-state fermentation (SSF) of Pleurotus eryngii. Agricultural waste including grape skin/seed and orange peel, rich in organic acids, were used instead of chemical reagents as mediators. The degradation products were subjected to liquid chromatography-mass spectrometry (LC-MS) analysis. Results: The results show that the enzymes manganese peroxidase and versatile peroxidase were associated with aniline blue degradation. The organic acid-rich agricultural waste (grape seed/skin, orange peels) serving as substitutes for chemical reagents as mediators reduced the cost of the mediator by 99.8% at best, and the 2 hr degradation rate of aniline blue was 97.4% and 95.8%, respectively, increased by at least 4.3%. The LC-MS analysis revealed that aniline blue was degraded into smaller compounds with significantly lower toxicity. Interpretation: Overall, the enzyme–organic acid-rich agricultural waste mediator (grape seed/skin) system effectively degraded aniline blue. This cheap and efficient method for aniline blue degradation can serve as an environmentally friendly technology to treat waste with waste. Key words: Agricultural waste, Aniline blue, Enzymatic degradation, Organic acids, Pleurotus eryngii

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Purification and characterization of extracellular laccase produced byCeriporiopsis subvermisporaand decolorization of triphenylmethane dyes

Cited by 29 publications

References 51 publications

Sustainable liquid supports for laccase immobilization and reuse: Degradation of dyes in aqueous biphasic systems

Sustainable liquid supports for laccase immobilization and reuse: Degradation of dyes in aqueous biphasic systems

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

New cost-effective mediator enhanced enzymatic degradation of aniline blue

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