Purification and characterization of enterocin 62-6, a two-peptide bacteriocin produced by a vaginal strain of<i>Enterococcus faecium</i>: Potential significance in bacterial vaginosis

DeZwaan, Diane C.; Mequio, Michael J.; Littell, Julia S.; Allen, Jonathan P.; Rossbach, Silvia; Pybus, Vivien

doi:10.3402/mehd.v19i4.7646

Cited by 2 publications

(4 citation statements)

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“…Plantacyclin B21AG was also shown to behave more hydrophobically on RP-HPLC column, requiring a high concentration (~80%) of ACN for elution. This behaviour has been observed for other circular bacteriocins like enterocin AS-48 [63] and enterocin 62-6 [59]. Finally, the protein sequence of the ORF corresponding to plantacyclin B21AG was shown to have similarity to other known circular bacteriocins.…”

Section: Resultssupporting

confidence: 59%

“…The observed behaviour of plantacyclin B21AG in this study was consistent with the properties of a circular bacteriocin. Firstly, plantacyclin B21AG could not be sequenced by N-terminal sequencing, similar to other circular bacteriocins, including enterocin 62-6 [59], lactocyclicin Q [60] and butyrivibriocin AR10 [61]. Secondly, plantacyclin B21AG showed some level of resistance to proteolytic enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…Secondly, plantacyclin B21AG showed some level of resistance to proteolytic enzymes. It has been reported that circular bacteriocins are generally more stable than classical bacteriocins [59, 62, 63]. They are less susceptible to digestion by endoproteinases and this probably increases their spectrum of activity [60, 64].…”

Section: Resultsmentioning

confidence: 99%

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Discovery and characterisation of novel circular bacteriocin plantacyclin B21AG fromLactobacillus plantarumB21

Golneshin

Gor

Vezina

et al. 2020

Preprint

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Lactobacillus plantarum B21 isolated from Vietnamese sausage (nem chua) has previously displayed broad antimicrobial activity against gram positive bacteria including foodborne pathogens Listeria monocytogenes and Clostridium perfringens. This study successfully identified the antimicrobial agent as plantacyclin B21AG, a 5668 Da circular bacteriocin demonstrating high thermostability, resistance to a wide range of pH, proteolytic resistance and temporal stability. We report a reverse genetics approach used to identify and characterise plantacyclin B21AG. The bacteriocin was purified from culture supernatant by a short process consisting of concentration, n-butanol extraction and cation exchange chromatography. A de novo peptide sequencing using LC-MS/MS techniques identified two putative peptide fragments which were mapped to the genome of Lactobacillus plantarum B21. This revealed an ORF corresponding to a putative circular bacteriocin with a 33-amino acid leader peptide and 58-amino acid mature peptide found on native plasmid pB21AG01. The corresponding gene cluster, consisted of seven genes associated with post-translational circularisation, immunity and secretion. The robust nature of plantacyclin B21AG, its antimicrobial activity and associated machinery for cyclisation make it an interesting biotechnological target for further development, and application as a food-safe antimicrobial.

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Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

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Discovery and characterisation of novel circular bacteriocin plantacyclin B21AG fromLactobacillus plantarumB21

Golneshin

Gor

Vezina

et al. 2020

Preprint

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“…Interestingly, another Ent. faecium strain EM297-BC-T6-1 was confirmed to harbour the structural genes for a recently identified bacteriocin, enterocin M (Marekova et al 2007), and also the genes for the two-component bacteriocin enterocin 62-6, which is closely related to enterocins L50A and L50B (Dezwaan et al 2007). MS analysis of the bacteriocins from Ent.…”

Section: Identification and Purification Of Bacteriocinsmentioning

confidence: 88%

Isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly Irish subjects

et al. 2012

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Aims: To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. Methods and Results: Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial-producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial-producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP-118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobialproducing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II.

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Purification and characterization of enterocin 62-6, a two-peptide bacteriocin produced by a vaginal strain ofEnterococcus faecium: Potential significance in bacterial vaginosis

Cited by 2 publications

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Discovery and characterisation of novel circular bacteriocin plantacyclin B21AG fromLactobacillus plantarumB21

Discovery and characterisation of novel circular bacteriocin plantacyclin B21AG fromLactobacillus plantarumB21

Isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly Irish subjects

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