Purification and characterization of chitosanase from Bacillus cereus D-11

Gao, Xing-Ai; Ju, Wan-Taek; Jung, Woo‐Jin; Park, Ro-Dong

doi:10.1016/j.carbpol.2007.09.025

Cited by 96 publications

(61 citation statements)

References 31 publications

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“…C-17 (Zhu et al, 2003) and Bacillus cereus D-11 (Gao et al, 2008). Peptone as a better nutrient source is needed by several microorganisms (Chen et al, 2009), but did not favor chitosanase production from S. albus YT2 in higher concentration.…”

Section: Box-behnken Design and Response Surface Methodsologymentioning

confidence: 91%

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Cheng

Wang

Hong

2012

Braz. J. Chem. Eng.

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-The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO 4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH 4 ) 2 SO 4 , 8 g/L urea, 1 g/L NH 4 Cl, 3 g/L KH 2 PO 4 , 1 g/L FeSO 4 ·7H 2 O, 2 g/L ZnSO 4 ·7H 2 O, 2 g/L CaCl 2 ·6H 2 O, 2 g/L MnSO 4 ·H 2 O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.

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Section: Box-behnken Design and Response Surface Methodsologymentioning

confidence: 91%

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Cheng

Wang

Hong

2012

Braz. J. Chem. Eng.

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“…A 72 h old culture of Serratia marcescens TKU011 presented a chitosanse activity around 0.025 U.mL -1 which wan after 30 minutes of a enzyme / substrate (1:5) reaction. The highest level of chitosanase activity in the Bacillus cereus D-11 culture after cultivation for 72 h was 4.85 U.mL -1 , but this activity was investigated after 30 minutes of enzyme / substrate (1:45) reaction (Gao et al 2008). Chitosanase produced by Microbacterium sp OU01 after 96 h had a maximum chitosanase activity of 118 U.mL -1 , after 15 minutes in contact with a 1% chitosan solution, the relation between enzyme and substrate was 1:10 (Sun et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…strains showed optimum activities at pH values between 4.0 and 8.0, the optimum temperatures of activity have been reported to be in the ranges 30-70°C (Kurakake et al 2000;Aktuganov et al 2003;Jo et al 2003;Choi et al 2004;Kim et al 2004;Su et al 2006;Gao et al 2008;Wang and Yeh, 2008). The effect of pH and temperature on the catalytic activity of chitosanase from P. ehimensis was studied by using soluble chitosan as substrate changing the pH and temperature during the assay.…”

Section: Enzyme Activity Optimal Conditionsmentioning

confidence: 99%

“…Enzymatic hydrolysis has some advantages in producing COS. Chitosanases can catalyze the hydrolysis under mild conditions (Kuo et al 2004;Ming et al 2006) Chitosanases have been recognized as enzymes that attack chitosan but not chitin and defined as the enzyme performing endohydrolysis of β-1,4-linkages between D-glucosamine residues in a partly acetylated chitosan (Su et al 2006). Chitosanase has been found in a variety of microorganisms, including various bacterial species (Kurakake et al 2000;Choi et al 2004;Kim et al 2004;Su et al 2006;Sun et al 2007;Zhu et al 2007;Gao et al 2008;Wang and Yeh, 2008;Wang et al 2008a;Wang et al 2008b) and fungi Ike et al 2007). Although, microbial chitosanases shows excellent performances in COS production, they are expensive to be utilized in large-scale industrial applications, because enzyme hydrolysis requires multi-steps, particularly, enzyme preparation and purification.…”

Section: Introductionmentioning

confidence: 99%

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Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis

Pagnoncelli

Araújo

Silva

et al. 2010

Braz. arch. biol. technol.

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“…Chitosanase (EC 3.2.1.132), an enzyme that specifically hydrolyzes chitosan, has been isolated from several bacteria and fungi. Some chitosanases have antifungal activity in vitro: a chitosanase from Bacillus cereus D-11 inhibits the hyphal growth of Rhizoctonia solani (Gao et al 2008); Amycolatopsis sp. CsO-2 produces a 27-kDa chitosanase CtoA that inhibits hyphal growth of Rhizopus oryzae (Saito et al 2009); chitosanase from Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Expression of a bacterial chitosanase in rice plants improves disease resistance to the rice blast fungus Magnaporthe oryzae

et al. 2011

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Plant fungal pathogens change their cell wall components during the infection process to avoid degradation by host lytic enzymes, and conversion of the cell wall chitin to chitosan is likely to be one infection strategy of pathogens. Thus, introduction of chitosan-degradation activity into plants is expected to improve fungal disease resistance. Chitosanase has been found in bacteria and fungi, but not in higher plants. Here, we demonstrate that chitosanase, Cho1, from Bacillus circulans MH-K1 has antifungal activity against the rice blast fungus Magnaporthe oryzae. Introduction of the cho1 gene conferred chitosanase activity to rice cells. Transgenic rice plants expressing Cho1 designed to be localized in the apoplast showed increased resistance to M. oryzae accompanied by increased generation of hydrogen peroxide in the infected epidermal cells. These results strongly suggest that chitosan exists in the enzyme-accessible surface of M. oryzae during the infection process and that the enhancement of disease resistance is attributable to the antifungal activity of the secreted Cho1 and to increased elicitation of the host defense response.

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Purification and characterization of chitosanase from Bacillus cereus D-11

Cited by 96 publications

References 31 publications

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis

Expression of a bacterial chitosanase in rice plants improves disease resistance to the rice blast fungus Magnaporthe oryzae

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