Purification and Characterization of Chitinase from Streptomyces sp. M-20

Kim, Kyoung Ja; Yang, Yu‐Guang; Kim, Jong Gi

doi:10.5483/bmbrep.2003.36.2.185

Cited by 76 publications

(62 citation statements)

References 17 publications

(15 reference statements)

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“…and they would be used in controlling plant pathogens. Kim et al (2003) also reported that the purified chitinase from Streptomyces sp. M-20 showed antifungal activity against Botrytis cinerea, and lysozyme activity against the cell wall of B. cinerea.…”

Section: Discussionmentioning

confidence: 96%

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Nguyen¹,

Naing²,

Lee³

et al. 2012

The Plant Pathology Journal

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The root rot of pepper (Capsicum annuum L.) caused by Phytophthora capsici is one of the most important diseases affecting this crop worldwide. This work presents the evaluation of the capacity of Streptomyces griseus H7602 to protect pepper plants against Phytophthora capsici and establishes its role as a biocontrol agent. In this study, we isolated an actinomycete strain H7602 from rhizosphere soil, identified it as Streptomyces griseus by 16S rRNA analysis and demonstrated its antifungal activity against various plant pathogens including P. capsici. H7602 produced lytic emzymes such as chitinase, β-1,3-glucanase, lipase and protease. In addition, crude extract from H7602 also exhibited destructive activity toward P. capsici hyphae. In the pot trial, results showed the protective effect of H7602 against pepper from P. capsici. Application of H7602 culture suspension reduced 47.35% of root mortality and enhanced growth of pepper plants for 56.37% in fresh root and 17.56% g in fresh shoot as compared to control, resulting in greater protection to pepper plants against P. capsici infestation. Additionally, the enzymatic activities, chitinase and β-1,3-glucanase, were higher in rhizosphere soil and roots of pepper plants treated with H7602 than other treated plants. Therefore, our results indicated a clear potential of S. griseus H7602 to be used for biocontrol of root rot disease caused by P. capsici in pepper.

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Section: Discussionmentioning

confidence: 96%

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Nguyen¹,

Naing²,

Lee³

et al. 2012

The Plant Pathology Journal

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“…M-20 was strongly inhibited with low concentrations of metallic ions. 41) The addition of CoSO 4 at 5 mM was found to enhance the activity of the enzyme, which increased by 325% as compared to that attained without Co 2þ . This result indicates that the enzyme requires Co 2þ for optimal activity.…”

Section: Chemical Modification and Effect Of Metallic Ions On The Actmentioning

confidence: 92%

Purification and Characterization of a Highly Thermostable Chitinase from the Stomach of the Red ScorpionfishScorpaena scrofawith Bioinsecticidal Activity toward Cowpea WeevilCallosobruchus maculatus(Coleoptera: Bruchidae)

Laribi-Habchi

Dziril

Badis

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…These enzymes have widespread applications in the fields of medicine (Kim et al 2003, Toharisman et al 2005, waste management (Odds 2003, Lopes et al 2008, biotechnology (Felse and Panda 1999a, b) and industry (Haki and Rakshit 2003). Chitinases are used for the mild degradation of chitin to prepare biologically active chitooligosaccharides and for the structural determination of the glycoprotein polysaccharide.…”

Section: Introductionmentioning

confidence: 99%

Purification, characterization and structural determination of chitinases produced by Moniliophthora perniciosa

Galante

Taranto

Koblitz

et al. 2012

An. Acad. Bras. Ciênc.

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The enzyme chitinase from Moniliophthora perniciosa the causative agent of the witches' broom disease in Theobroma cacao, was partially purified with ammonium sulfate and filtration by Sephacryl S-200 using sodium phosphate as an extraction buffer. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes were obtained: ChitMp I, ChitMp II, ChitMp III and ChitMp IV. ChitMp I had an optimum temperature at 44-73°C and an optimum pH at 7.0-8.4. ChitMp II had an optimum temperature at 45-73°C and an optimum pH at 7.0-8.4. ChitMp III had an optimum temperature at 54-67°C and an optimum pH at 7.3-8.8. ChitMp IV had an optimum temperature at 60°C and an optimum pH at 7.0. For the computational biology, the primary sequence was determined in silico from the database of the Genome/Proteome Project of M. perniciosa, yielding a sequence with 564 bp and 188 amino acids that was used for the three-dimensional design in a comparative modeling methodology. The generated models were submitted to validation using Procheck 3.0 and ANOLEA. The model proposed for the chitinase was subjected to a dynamic analysis over a 1 ns interval, resulting in a model with 91.7% of the residues occupying favorable places on the Ramachandran plot and an RMS of 2.68. Key words:Chitinase, Moniliophthora perniciosa, kinetic characterization, purification, isoenzymes, heat stability, 3D structure, comparative modeling.

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Purification and Characterization of Chitinase from Streptomyces sp. M-20

Cited by 76 publications

References 17 publications

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Purification and Characterization of a Highly Thermostable Chitinase from the Stomach of the Red ScorpionfishScorpaena scrofawith Bioinsecticidal Activity toward Cowpea WeevilCallosobruchus maculatus(Coleoptera: Bruchidae)

Purification, characterization and structural determination of chitinases produced by Moniliophthora perniciosa

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