Purification and characterization of casein kinase II from lactating rabbit mammary gland

Mitev, Vanio; Pauloin, Alain; Houdebiné, Louis‐Marie

doi:10.1016/0020-711x(94)90167-8

Cited by 3 publications

(3 citation statements)

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“…31, it was clear that the peaks of activity toward phosvitin corresponded with the presence of the CK 2 enzyme in fractions. Elution of the CK 2 holoenzyme from the Mono Q column was mainly between 0.47-0.56 M NaC1, which is consistent with the elution profile reported in other systems (Daya-Makin et al, 1994;Hei et al, 1994;Mitev et al, 1994;Sutherland et al, 1994). Furthermore, this peak of phosvitin phosphotransferase activity was abolished with 10 pg/ml heparin, which is a potent inhibitor of CK 2 (data not shown).…”

Section: Resultssupporting

confidence: 88%

“…For Western blotting, SDS-polyacrylamide gel electrophoresis was performed on 1.5 mm thick gels (4% stacking gels and 11% separating gels) using a similar buffer system described by Laemmli (1970). Mono Q fractions #35-43 were used in electrophoresis because CK 2 elutes in these fractions (Daya-Makin et al, 1994;Hei et al, 1994;Mitev et al, 1994;Sutherland et al, 1994). Fractionated samples were reconstituted in sample buffer L1.25 mM Tris HC1 (pH 6.8) 4% SDS, 0.01% bromophenol blue, 10% 2-mercaptoethanol and 20% glycerol] at a ratio of 2:l for a total volume of 120 pl per lane, where as unfractionated supernatant was loaded at 100 pg protein per lane at the same volume.…”

Section: Methodsmentioning

confidence: 99%

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Changes in casein kinase 2 activity during development of the secondary palate in the hamster

et al. 1996

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Background Casein kinase 2 (CK 2) is a serine/threonine kinase that has been ubiquitously conserved in all eukaryotic cells. The exact functions of this enzyme have not yet been clarified; however, studies have repeatedly suggested that it may play crucial roles in the regulation of cell proliferation. During the formation of the secondary palate in the hamster, bursts of cell proliferation occur during the initial half of vertical shelf development, which decrease during the subsequent steps of palate morphogenesis, thus indicating that the cell cycle in the developing vertical palate may be tightly regulated. Methods In the present study, palatal shelves were dissected at 12‐hour intervals between days 10 and 12 of gestation, which is the period of vertical shelf development in the hamster. The palates were homogenized and cleared by ultracentrifugation and the resultant supernatants were fractionated on a Mono Q column by fast protein liquid chromatography. Results Using phosvitin as a substrate, the phosphotransferase activity in the fractionated samples decreased steadily from days 10 to 11, increased to a fivefold peak on day 11:12, and then decreased on day 12 of gestation. Western blot analysis using two CK 2 specific antibodies demonstrated that both the 42‐kDa (α) and the 38‐kDa (α′) subunits of the CK 2 holoenzyme were found throughout the formation of the vertical palatal shelves in the hamster. The amount of α and α′ subunits appears to remain constant, which suggested that the differential activity of the CK 2 enzyme may be due to posttranslational modifications. CK 2 activity correlated well with DNA synthesis (i.e., cell proliferation) rates from days 10 to 11, but not from days 11 to 12 of gestation. Conclusions It is proposed that the activity of CK 2 may regulate the rate of cell proliferation by stimulation of progression through G1 phase of the cell cycle and may also relate to the effects of various growth factors during the vertical development of mammalian palate. © 1996 Wiley‐Liss, Inc.

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Section: Resultssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Changes in casein kinase 2 activity during development of the secondary palate in the hamster

et al. 1996

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“…Notably, CSNK1A1 was the highest ranking hit from siRNA screen 3, and 2 additional casein kinase (CSNK) family members, CSNK1G2 and CSNK2A2, were found to be negative modulators of autophagy by siRNA screening ( Table S1D ), suggesting casein kinases as possible targets. Currently available CSNK inhibitors include: 1) heparin, which has been reported to inhibit both CSNK1A1 21 and CSNK2A2, 22 2) ionomycin, which has been reported to inhibit CSNK1A1, 23 and 3) suramin, 22 2-aminopurine, 24 and 6-dimethyladenine, 24 which have been reported to inhibit CSNK2A2. Hence, those agents alone or in combination with other drugs could be used to test the hypothesis that stimulating autophagy through inhibition of casein kinases results in cancer cell death.…”

Section: Introductionmentioning

confidence: 99%

A curated census of autophagy-modulating proteins and small molecules

et al. 2014

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Autophagy, a programmed process in which cell contents are delivered to lysosomes for degradation, appears to have both tumor-suppressive and tumor-promoting functions; both stimulation and inhibition of autophagy have been reported to induce cancer cell death, and particular genes and proteins have been associated both positively and negatively with autophagy. To provide a basis for incisive analysis of those complexities and ambiguities and to guide development of new autophagy-targeted treatments for cancer, we have compiled a comprehensive, curated inventory of autophagy modulators by integrating information from published siRNA screens, multiple pathway analysis algorithms, and extensive, manually curated text-mining of the literature. The resulting inventory includes 739 proteins and 385 chemicals (including drugs, small molecules, and metabolites). Because autophagy is still at an early stage of investigation, we provide extensive analysis of our sources of information and their complex relationships with each other. We conclude with a discussion of novel strategies that could potentially be used to target autophagy for cancer therapy.

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Stathmin gene expression in mammary gland and in Nb₂ cells

Puissant

Mitev

Lemnaouar

et al. 1995

Biology of the Cell

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Mammary gland growth occurs essentially during pregnancy and induction of milk synthesis is triggered at parturition. Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for the multiplication of the rat lymphoid Nb2 cells. Stathmin is an ubiquitous and highly conserved phosphoprotein which seems to be involved in the intracellular mechanisms which trigger cell multiplication and differentiation. In the present study, the concentration of stathmin mRNA has been evaluated during the pregnancy-lactation-weaning cycle in mouse and rabbit. Stathmin mRNA appeared at its highest level during pregnancy and it was almost undetectable during lactation. Prolactin injected into mid-pregnant rabbits induced milk synthesis and this effect was not accompanied by any modification of stathmin mRNA concentration. In cultured primary rabbit mammary cells, prolactin induced casein gene expression without any alteration of stathmin mRNA concentration. In Nb2 cells, prolactin induced a progressive increase of stathmin mRNA concentration. This effect was not significant until after 4 h of prolactin action. These data suggest that stathmin is involved in mammary and Nb2 cell multiplication but may not be necessary for mammary cell differentiation.

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Purification and characterization of casein kinase II from lactating rabbit mammary gland

Cited by 3 publications

References 30 publications

Changes in casein kinase 2 activity during development of the secondary palate in the hamster

Changes in casein kinase 2 activity during development of the secondary palate in the hamster

A curated census of autophagy-modulating proteins and small molecules

Stathmin gene expression in mammary gland and in Nb₂ cells

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Purification and characterization of casein kinase II from lactating rabbit mammary gland

Cited by 3 publications

References 30 publications

Changes in casein kinase 2 activity during development of the secondary palate in the hamster

Changes in casein kinase 2 activity during development of the secondary palate in the hamster

A curated census of autophagy-modulating proteins and small molecules

Stathmin gene expression in mammary gland and in Nb2 cells

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Product

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Stathmin gene expression in mammary gland and in Nb₂ cells