Purification and characterization of calcium-binding conchiolin shell peptides from the mollusc,Haliotis rufescens, as a function of development

Cariolou, Marios A.; Morse, Daniel E.

doi:10.1007/bf00691002

Cited by 102 publications

(62 citation statements)

References 27 publications

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“…This same trend was reported by Jurkiewicz-Karnkowska (2002), who studied six species of molluscs (three gastropod and three bivalves) from Poland and found concentrations of P varying from 0.63 and 2.68% dw. Shells of molluscs also contain phosphorus (e.g., calcium phosphate), and the phosphorylated protein conchiolin is abundant in their soft tissues, playing a key role in the aggregation of minerals and organic compounds to produce shells (Cariolou & Morse, 1988). Thus, the presence of conchiolin is crucial to raise the tissue concentrations of P in molluscs.…”

Section: Nitrogen and Phosphorusmentioning

confidence: 99%

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Diniz

Barbarino

Oiano-Neto

et al. 2014

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ABSTRACT. The chemical profiles of Desmapsamma anchorata, Hymeniacidon heliophila (Porifera), Bunodosoma caissarum, Renilla muelleri (Cnidaria), Aplysia brasiliana, Eledone massyae, Isognomon bicolor (Mollusca), Echinaster brasiliensis, Echinometra lucunter, Holothuria grisea, Lytechinus variegatus (Echinodermata), and Phallusia nigra (Chordata) were determined. Hydrosoluble protein was the most abundant class of substances for all species, except for the ascidian Phallusia nigra, in which the carbohydrate content was higher. The percentages of hydrosoluble protein (dry weight, dw) varied widely among the invertebrates, ranging from 5.88% (R. muelleri) to 47.6% (Eledone massyae) of the dw .The carbohydrate content fluctuated from 1.3% (R. muelleri) to 18.4% (Aplysia brasiliana) of the dw. For most of the species, lipid was the second most abundant class of substances, varying from 2.8% (R. muelleri) to 25.3% (Echinaster brasiliensis) of the dw. Wide variations were also found for the invertebrates nitrogen content, with the lowest value recorded in the cnidarian R. muelleri (2.02% of the dw) and the highest in the molluscan E. massyae (12.7% of the dw). The phosphorus content of the dw varyed from 0.24% (R. muelleri) to 1.16% (E. massyae). The amino acid composition varied largely among the species, but for most of the species glycine, arginine, glutamic acid, and aspartic acid were the most abundant amino acids, with histidine and tyrosine among the less abundant amino acids. The actual content of total protein in the samples was calculated by the sum of amino acid residues, establishing dw values that fluctuated from 11.1% (R. muelleri) to 66.7% (E. massyae). The proteinaceous nitrogen content was high in all species, with an average value of 97.3% of the total nitrogen. From data of total amino acid residues and total nitrogen, specific nitrogen-to-protein conversion factors were calculated for each species. The nitrogen-to-protein conversion factors ranged from 5.10 to 6.15, with an overall average of 5.45. The use of the specific nitrogen-to-protein conversion factors established here is recommended, since it would yield more accurate determinations of total protein in the species tested in this study. Keywords: nitrogen-to-protein conversion factors, total protein, amino acid composition, lipid, carbohydrate, tropical Atlantic. Composición proximal de invertebrados marinos de aguas costeras tropicales,con énfasis en la relación entre los contenidos de nitrógeno y proteína RESUMEN. Se determinaron los perfiles químicos de Desmapsamma anchorata, Hymeniacidon heliophila (Porifera), Bunodosoma caissarum, Renilla muelleri (Cnidaria), Aplysia brasiliana, Eledone massyae, Isognomon bicolor (Mollusca), Echinaster brasiliensis, Echinometra lucunter, Holothuria grisea, Lytechinus variegatus (Echinodermata), y Phallusia nigra (Chordata). La clase más abundante de sustancias para todas las especies fueron las proteínas hidrosolubles, excepto para la ascidia Phallusia nigra, en la que el contenido de carbohidratos fue ...

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Section: Nitrogen and Phosphorusmentioning

confidence: 99%

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Diniz

Barbarino

Oiano-Neto

et al. 2014

lajar

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“…The organic molecules can be extracted with aqueous and organic solvents. The water-soluble organic matrix dictates which calcium carbonate crystal structure is formed and when it is deposited (Cariolou & Morse, 1988). The bulk of the watersoluble biopolymer is thought to consist of a complex mixture of proteins and peptides (Kono et al, 2000;Weiss et al, 2000).…”

Section: Biological Action Of Nacre Extractsmentioning

confidence: 99%

Nacre, a Natural Biomaterial

Rousseau¹

2011

Biomaterials Applications for Nanomedicine

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“…-binding and other strongly anionic proteins blue. [31][32][33] Stains-All stained the AP8 proteins blue ( Figure 1, lane 4), with staining intensity relatively higher than Coomassie Brilliant Blue (Figure 1, lane 3), demonstrating that the proteins are acidic and likely to bind Ca 2+ ions. Although the AP8 proteins were the only ones observed in gels of the ammonium sulfate supernatant, the proteins were further purified for additional analyses and crystal growth experiments.…”

Section: Ap8 Protein Purification and Biochemical Characterizationmentioning

confidence: 99%

CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

et al. 2005

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Acidic proteins from many biogenic minerals are implicated in directing the formation of crystal polymorphs and morphologies. We characterize the first extremely acidic proteins purified from biomineralized aragonite. These abalone nacre proteins are two variants of 8.7 and 7.8 kDa designated AP8 (for aragonite proteins of approximately 8 kDa). The AP8 proteins have compositions dominated by Asx (∼35 mol %) and Gly (∼40 mol %) residues, suggesting that their structures have high Ca 2+ -binding capacity and backbone flexibility. The growth of asymmetrically rounded CaCO 3 crystals in the presence of AP8 reveals that both proteins preferentially interact with specific locations on the crystal surface. In contrast, CaCO 3 crystals grown with nacre proteins depleted of AP8 retain the morphology of unmodified calcite rhombohedra. Our observations thus identify sites of protein-mineral interaction and provide evidence to support the long-standing theory that acidic proteins are more effective crystal-modulators than other proteins from the same biomineralized material.

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Purification and characterization of calcium-binding conchiolin shell peptides from the mollusc,Haliotis rufescens, as a function of development

Cited by 102 publications

References 27 publications

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Nacre, a Natural Biomaterial

CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

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Purification and characterization of calcium-binding conchiolin shell peptides from the mollusc,Haliotis rufescens, as a function of development

Cited by 102 publications

References 27 publications

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Proximate composition of marine invertebrates from tropical coastal waters, with emphasis on the relationship between nitrogen and protein contents

Nacre, a Natural Biomaterial

CaCO3Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

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Product

Resources

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CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology