Purification and Characterization of Bot33: A Non-Toxic Peptide from the Venom of Buthus occitanus tunetanus Scorpion

ElFessi, Rym; Khamessi, Oussema; Srairi-Abid, Najet; Sabatier, Jean‐Marc; Tytgat, Jan; Peigneur, Steve; Kharrat, Riadh

doi:10.3390/molecules27217278

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…To maximize the purification process, RP-HPLC can be complemented with other techniques. In certain cases, it may be utilized as the final step to achieve the highest level of purification, as demonstrated in the isolation of neurotoxic peptides from Iranian Hemiscorpius lepturus venom and the isolation of a nontoxic peptide (Bot33) from Buthus occitanus tunetanus [ 26 , 51 ]. Most commonly, during scorpion venom analysis, RP-HPLC is followed by a mass spectrometric analysis of the fractions.…”

Section: Methods Used For Separation Of Venom Complex Mixturesmentioning

confidence: 99%

Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods

Nasr,

Borges,

Sahyoun

et al. 2023

Antibiotics

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Scorpion venoms have long captivated scientific researchers, primarily due to the potency and specificity of the mechanism of action of their derived components. Among other molecules, these venoms contain highly active compounds, including antimicrobial peptides (AMPs) and ion channel-specific components that selectively target biological receptors with remarkable affinity. Some of these receptors have emerged as prime therapeutic targets for addressing various human pathologies, including cancer and infectious diseases, and have served as models for designing novel drugs. Consequently, extensive biochemical and proteomic investigations have focused on characterizing scorpion venoms. This review provides a comprehensive overview of the key methodologies used in the extraction, purification, analysis, and characterization of AMPs and other bioactive molecules present in scorpion venoms. Noteworthy techniques such as gel electrophoresis, reverse-phase high-performance liquid chromatography, size exclusion chromatography, and “omics” approaches are explored, along with various combinations of methods that enable bioassay-guided venom fractionation. Furthermore, this review presents four adapted proteomic workflows that lead to the comprehensive dissection of the scorpion venom proteome, with an emphasis on AMPs. These workflows differ based on whether the venom is pre-fractionated using separation techniques or is proteolytically digested directly before further proteomic analyses. Since the composition and functionality of scorpion venoms are species-specific, the selection and sequence of the techniques for venom analyses, including these workflows, should be tailored to the specific parameters of the study.

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Section: Methods Used For Separation Of Venom Complex Mixturesmentioning

confidence: 99%

Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods

Nasr,

Borges,

Sahyoun

et al. 2023

Antibiotics

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“…Four fractions, M1, M2, BotG50 and M3, were eluted [13]. The BotG50 fraction was known to contain peptides active on ion channels and endowed with pharmacological activities [14][15][16]. Accordingly, this fraction was selected for further purification.…”

Section: Screening For Anti-ndv Active Molecules From Bot Scorpion Venommentioning

confidence: 99%

BotCl, the First Chlorotoxin-Like Peptide Inhibiting Newcastle Disease Virus: The Emergence of a New Scorpion Venom AMPs Family

Jlassi,

Mekni-Toujani,

Ferchichi

et al. 2023

Molecules

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Newcastle disease virus (NDV) is one of the most serious contagions affecting domestic poultry and other avian species. It causes high morbidity and mortality, resulting in huge economic losses to the poultry industry worldwide. Despite vaccination, NDV outbreaks increase the need for alternative prevention and control means. In this study, we have screened fractions of Buthus occitanus tunetanus (Bot) scorpion venom and isolated the first scorpion peptide inhibiting the NDV multiplication. It showed a dose dependent effect on NDV growth in vitro, with an IC50 of 0.69 µM, and a low cytotoxicity on cultured Vero cells (CC50 > 55 µM). Furthermore, tests carried out in specific pathogen-free embryonated chicken eggs demonstrated that the isolated peptide has a protective effect on chicken embryos against NDV, and reduced by 73% the virus titer in allantoic fluid. The N-terminal sequence, as well as the number of cysteine residues of the isolated peptide, showed that it belongs to the scorpion venom Chlorotoxin-like peptides family, which led us to designate it “BotCl”. Interestingly, at 10 µg/mL, BotCl showed an inhibiting effect three times higher than its analogue AaCtx, from Androctonus australis (Aa) scorpion venom, on NDV development. Altogether, our results highlight the chlorotoxin-like peptides as a new scorpion venom AMPs family.

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Model Organisms Used in Aquatic Toxicology

Parlak

2024

Springer Water

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Purification and Characterization of Bot33: A Non-Toxic Peptide from the Venom of Buthus occitanus tunetanus Scorpion

Cited by 4 publications

References 44 publications

Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods

Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods

BotCl, the First Chlorotoxin-Like Peptide Inhibiting Newcastle Disease Virus: The Emergence of a New Scorpion Venom AMPs Family

Model Organisms Used in Aquatic Toxicology

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