Purification and characterization of an O-acetylsialic acid-specific lectin from a marine crab Cancer antennarius.

Ravindranath, Mepur H.; Higa, H H; Cooper, Edwin L.; Paulson, James C.

doi:10.1016/s0021-9258(17)39429-2

Cited by 151 publications

(18 citation statements)

References 49 publications

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“…WG/~s lack of activity may be attributable to its relatively restricted range in binding sialyloligosaceharides. For example, WGA does not bind to N-glycolyl sialic acid, while LA recognizes this form of sialic acid as well as the more prevalent N-acetyl form (Bhavanandan and Katlic, 1979;Ravindranath et al, 1985). Furthermore, WGA binds to sialylated neo-lacto glycosphingolipids but fails to recognize brain gangliosides (Molin et al, 1986), while LA binds to both glycolipid classes (True, D. D., B.…”

Section: Discussionmentioning

confidence: 99%

“…The availability of siaiic acidspecific lectins provided the opportunity for further study of the involvement of siaiyloligosaccharides in the adhesive function of HEV. LA, isolated from the slug Limaxflavus, is highly specific for sialic acid in many of its naturally occurring forms and linkages (Miller, 1987;Miller et ai., 1982;Ravindranath et al, 1985;Schulte et ai., 1984;Wagner and Roth, 1988). LA and a series of other lectins, representing a wide range of carbohydrate-binding specificities (see Materials and Methods), were compared for their ability to inhibit the adhesive function of HEV in tissue sections.…”

Section: Effects Of Lectins On Lymphocyte Attachment Sites Of Hevmentioning

confidence: 99%

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Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.

True

Singer

Lasky

et al. 1990

The Journal of Cell Biology

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Abstract. The entry of blood-borne lymphocytes into most secondary lymphoid organs is initiated by a highly specific adhesive interaction with the specialized cuboidal endothelial cells of high endothelial venules (HEV). The adhesive receptors on lymphocytes that dictate interactions with HEV in different lymphoid organs are called homing receptors, signifying their critical role in controlling organ-selective lymphocyte migration. Considerable work has established that the mouse peripheral lymph node homing receptor (pnHR), defined by the mAb MEL-14, functions as a lectin-like adhesive protein. We have previously shown that sialidase treatment of peripheral lymph node (PN) HEV abrogates lymphocyte attachment to the HEV both in vivo and in vitro. We extend this evidence by demonstrating that L/max agglutinin (LA), a sialic acid-specific lectin, when reacted with HEV exposed in cryostat-cut tissue sections, blocks lymphocyte attachment to PN HEV and, unexpectedly, to the HEV of Peyer's patches (PP) as well. Using a recombinant form of the pnHR as a histochemical probe for its cognate adhesive site (HEV-ligand) on PN HEV, we demonstrate that both sialidase and L/max agglutinin functionally inactivate this ligand. It is concluded that the requirement for sialic acid is at the level of the pnHR interaction with its HEV ligand. A distinct sialyloligosaccharide may encode the recognition determinant of a PP HEV ligand.

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Section: Discussionmentioning

confidence: 99%

Section: Effects Of Lectins On Lymphocyte Attachment Sites Of Hevmentioning

confidence: 99%

Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.

True

Singer

Lasky

et al. 1990

The Journal of Cell Biology

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“…They exhibit great diversity in sugar binding specificity. Thus, the lectins are known to specifically recognize the whole sugar, a specific part of a sugar, a sequence of sugars, or their glycosidic linkages (Ravindranath et al, 1985;Murali et al, 1999). Besides, a few studies have demonstrated that the lectins especially from diverse animal sources can additionally recognize certain non-carbohydrate ligands including peptide motif and even simple chemicals containing appropriate determinant structures (Gabius, 1994;Kawagishi et al, 1994;Gokudan et al, 1.6.…”

Section: Binding Specificitymentioning

confidence: 99%

An overview on human serum lectins

Manikandan¹,

Manikandan

Arumugam

et al. 2020

Heliyon

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An extensive literature survey done on the various naturally occurring lectins in human serum upon its salient features such as methods of detection, level and sites of synthesis, binding specificity, cation dependency, modes of isolation, molecular and functional characterization way back from 1930s to till date was presented in a tabulated section. In addition, the generation of lectin and other immune molecules in vertebrates upon treatment with exogenous elicitors has also been framed in a tabular form. Furthermore, ANEW lectin induced in human serum for the very first time by an exogenous elicitor was detected, isolated and characterized by us whose features are also tabulated explicitly.

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“…On the other hand, sialic addspecific lectins isolated from Limulus polyphemus (Marchalonis & Edelman, 1968) or Limax flavus (Miller et al, 1982) have been widely used for cytochemical labelling (Mazzuca et al, 1977;Yamada & Shimizu, 1979;Muresan et al, 1982a, b;Roth et aI., 1984Roth et aI., , 1985Roth et aI., , 1986Charest & Roth, 1985;Hedman et al, 1986). However, both lectins have a rather broad reactivity with the different forms of sialic acid (Miller et al, 1982;Ravindranath et al, 1985) and the Limulus polyphemus lectin has, in addition, a binding site for phosphorylcholine (Mohan et al, 1983). Wheatgerm agglutinin, although often used in histochemical studies is neither a specific nor sensitive probe for sialic acid, but rather for oligomers of N-acetylglucosamine (Goldstein & Poretz, 1986).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, lectins with a narrow specificity for sialic acid have been purified: the Cancer antennaria lectin recognizing 9-0-, and to a lesser extent 5-O-acetylated neuraminic acid (Ravindranath et al, 1985), the Achatina futica lectin reacting with 9-O-acetylated neuraminic acid (Mandal & Basu, 1987) and the Sambucus nigra L. (SNA, elderberry bark) lectin recognizing neuraminic acid ct2,6-1inked to galactose (Gal) or N-acetylgalactosamine (GalNAc) (Shibuya et al, 1987a). Here, we report the preparation of an elderberry bark lectin-gold (SNA-g) complex and its use for light and electron microscopic postembedding labelling as well as detection of nitrocellulose-immobilized glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

Elderberry bark lectin-gold techniques for the detection of Neu5Ac (?2,6) Gal/GalNAc sequences: applications and limitations

Taatjes¹,

Roth²,

Peumans

et al. 1988

Histochem J

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The lectin from the elderberry (Sambucus nigra L.) bark, shown to recognize the sequence neuraminic acid (alpha 2,6) galactose/N-acetylgalactosamine, was applied for detecting binding sites in Lowicryl K4M sections by light and electron microscopy. The lectin was used either directly complexed to colloidal gold or in a two-step cytochemical affinity technique. The lectin-gold complex proved to be superior and thus was extensively tested on rat liver, kidney and hepatoma cells as well as on sheep and bovine submandibular glands. Controls to establish specificity of lectin-gold binding included sugar and glycoprotein inhibition tests and enzymic removal of sialic acid. In agreement with biochemical data demonstrating the potentiating effect of sialic acid on the binding of the lectin to oligosaccharides, enzymic removal of sialic acid from liver sections resulted in abolition of lectin staining. However, in the submandibular glands, neuraminidase pretreatment of the sections had no effect on the subsequent lectin-gold binding. In rat kidney some structures became negative while others retained the lectin-gold staining due to binding to penultimate N-acetylgalactosamine exposed after sialic acid removal. In line with this, spot blot analysis demonstrated that the lectin-gold complex reacted with both fetuin and asialofetuin. Taken together, these results suggest that, for cytochemical staining, the sialic acid and the galactose/N-acetylgalactosamine lectin combining subsites of Sambucus nigra L. lectin are equally reactive with cellular glycoconjugates and that neuraminidase predigestion of tissue sections is of utmost importance to ensure specificity of staining for the sequence neuraminic acid (alpha 2,6) galactose/N-acetylgalactosamine.

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Purification and characterization of an O-acetylsialic acid-specific lectin from a marine crab Cancer antennarius.

Cited by 151 publications

References 49 publications

Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.

Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.

An overview on human serum lectins

Elderberry bark lectin-gold techniques for the detection of Neu5Ac (?2,6) Gal/GalNAc sequences: applications and limitations

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