Purification and characterization of an antitumor polysaccharide from Portulaca oleracea L.

Shen, Huan; Tang, Guo Qing; Zeng, Guang; Yang, Yue; Cai, Xueli; Li, Dongli; Liu, Hongchen; Zhou, Nan

doi:10.1016/j.carbpol.2012.11.107

Cited by 114 publications

(53 citation statements)

References 22 publications

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“…Alpha-tocopherol, ascorbic acid, beta-carotene and glutathione in leaves of purslane exhibited higher amount than did in spinach (Simopoulos et al, 1992). Purification and characterization of an antitumor polysaccharide from purslane in rats model studied by (Shen et al, 2013) showed that purslane enhanced animal's immune responses including an increase in the number of white blood cell (WBC) and CD4(+) Tlymphocytes. Anti-inflammatory properties of ethanolic extract of phenolics from the aerial parts (dried leaves and stem) was compared with the synthetic drug, diclofenac sodium as the active control in mice model for eight www.ccsenet.org/jfr Journal of Food Research Vol.…”

Section: Other Bioactives In Purslanementioning

confidence: 97%

Redefining Unusable Weeds to Beneficial Plants: Purslane as a Powerful Source of Omega-3 for the Future

Agil¹,

Gilbert²,

Tavakoli³

et al. 2015

JFR

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With global consumer demand shifting towards the consumption of healthier foods, it is crucial to discover new sources of edible plants with high nutritional value and low cost. Unique weeds such as purslane have the potential to be used as an untapped source of unconventional food with diverse nutrients and beneficial bioactive properties. Inflammation can cause oxidative stress related diseases including cardiovascular disorders, aging and cancer. One key nutrient of purslane is omega-3 with potential of inhibitory properties against inflammatory and estrogenic mediators. Purslane is known to be a rich source of α-linolenic acid, 18:3 ω-3, an essential fatty acid, carotenes, antioxidants and minerals. However, the precise mechanism of action of its individual components in disease prevention is unknown. This review provides a summary on the role of purslane bioactives, particularly omega-3 fatty acids as one of purslane's main constituents with potential of anti-inflammatory and anti-estrogenic properties. The discovery of new sources of plants rich in omega-3 fatty acids may be a useful strategy in utilizing natural alternative sources of foods that can enhance human health and wellbeing.

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Section: Other Bioactives In Purslanementioning

confidence: 97%

Redefining Unusable Weeds to Beneficial Plants: Purslane as a Powerful Source of Omega-3 for the Future

Agil¹,

Gilbert²,

Tavakoli³

et al. 2015

JFR

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“…In this study, the polysaccharides were extracted from Purslane by ultrasonic method and purified by DEAE-Cellulose anion-exchange and Sephadex gel-permeation chromatography. Three fractions were obtained and named POL1, POL2 and POL3, with molecular weights of 18Kd, 55kd and 108kd, respectively, which were different from Shen's report that the molecular weight of polysaccharides from Purslane was 26kDa by the method of gel permeation chromatography [19]. The possible reasons for the molecular weight difference of polysaccharides were, on the one hand, the source of Purslane used in the study and the kind of polysaccharides were different; on the other hand, the eluent is different during purification.…”

Section: Discussionmentioning

confidence: 99%

Extraction, purification and antibacterial activity of polysaccharides from Purslane

Gu¹,

Shu²

2017

Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)

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Abstract. In this study, the polysaccharides from Purslane were extracted by ultrasonic method and its content was measured by the phenol-sulfuric acid method using glucose as the standard. The crude polysaccharides were collected and purified by DEAE-Cellulose anion-exchange and Sephadex gel-permeation chromatography. Three fractions were obtained and named POL1, POL2 and POL3, respectively. The molecular weights of these three fractions were measured by Sephadex using dextran standard curve. Escherichia coli, Acetic acid bacteria, Saccharomycetes and Aspergillus Niger were used to study the antibacterial activity of the polysaccharides. The results showed that molecular weights of POL1, POL2 and POL3 were 18Kd, 55kd and 108kd, respectively. The polysaccharides had inhibitory effect on microorganisms, and the inhibited order of microorganisms was: Saccharomycetes>Acetic acid bacteria>Aspergillus Niger>Escherichia coli. In addition, the order of antibacterial activities of these three fractions on microorganisms was: POL3>POL2b>POL1b. It could be concluded that three fractions were obtained by purification. These three fractions had inhibitory effect on Saccharomycetes, Acetic acid bacteria, Aspergillus Niger and Escherichia coli. The antibacterial activity of polysaccharides from Purslane against Saccharomycetes was the strongest, while the weakest against Escherichia coli. In addition, POL3 was found to be the most effective against the tested microorganisms among the three fractions.

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“…Antitumor effects in vivo (Shen et al, 2013) and in vitro (Tian et al, 2013) model and several powerful pharmaceutical activities for example soothing, hepatoprotective, neuropharmacological, antiinflammatory, antidiabetic, bronchodilator, antihypertensive, antioxidant and containing the noradrenaline hormone has been reported (Amirul Alam et al, 2014). Noradrenaline and Dopamine are the major catecholamine bioactive compounds present in P. oleracea L. as an immune system modulator for treatment of shock (Chen et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Ghorbani

Omidi

et al. 2015

Turkish JAF Sci.Tech.

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Common purslane (Portulaca oleracea L.) is an annual plant as one of the natural sources for noradrenaline hormone. In this research, hairy root culture of purslane was established by using Agrobacterium rhizogenes strain ATCC 15834. In the following, Box-Behnken model of response surface methodology (RSM) was employed to optimize B5 medium for the growth of P. oleracea L. hairy root line. According to the results, modelling and optimization conditions, including sucrose, CaCl 2 .H 2 O, H 2 PO 4 and NO 3 -/NH 4 + concentrations on maximum dry weight (0.155 g) and noradrenaline content (0.36 mg.g -1 DW) was predicted. These optimal conditions predicted by RSM were confirmed the enhancement of noradrenaline production as an application potential for production by hairy root cultures.

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Purification and characterization of an antitumor polysaccharide from Portulaca oleracea L.

Cited by 114 publications

References 22 publications

Redefining Unusable Weeds to Beneficial Plants: Purslane as a Powerful Source of Omega-3 for the Future

Redefining Unusable Weeds to Beneficial Plants: Purslane as a Powerful Source of Omega-3 for the Future

Extraction, purification and antibacterial activity of polysaccharides from Purslane

Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

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