Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

Abstract

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was 57.16ºC. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ·OH assays, and its antioxidant activity was stable at 30-50ºC in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

Combination of chick embryo and nutrient mixture prevent D-galactose-induced cognitive deficits, immune impairment and oxidative stress in aging rat model

“…They have been shown for its biological activities, including anti-fatigue, antioxidant activity and improving immunity 11 . In addition, as natural dietary supplement, chick embryo eggs are considered as the nutrition, have quality supplement and illness treatment in China 12 . Studies have shown that chick embryo contains stem cell factor (SCF), nerve growth factor (NGF), epidermal growth factor (EGF), interleukin-4 (IL-4) and interleukin-2 (IL-2) 13 , 14 .…”

Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs

“… Abeyrathne et al (2014) showed that peptide derived from ovalbumin were hydrolyzed by alcalase and trypsin showed strong iron- and copper-binding capacities and antioxidant capacity. The sequence of antioxidant protein obtained from fertilized eggs on day 15 exhibited highly similar to ovalbumin ( Yang et al , 2016 ). Thus, we suspected that the purified protein in this study was a new antioxidant protein.…”

The Functions of Chicken Embryo Extract

“…Chicken embryo eggs are regarded as a high-quality natural nutrition supplement and sickness cure in China (where they are known as Mao Dan) and other Southeast Asian nations [1]. Pregnant women, the elderly and even cancer patients are advised to consume it [2].…”