Purification and characterization of an acid phosphatase from cell membrane fraction of Lactococcus lactis ssp lactis 303

Effects of dissolved oxygen on survival and immune responses of scallop (Chlamys farreri Jones et Preston)

“…Phagocytosis is considered an important way to control and eliminate invaders. It is well known that the life cycle, food supply, diseases, pollutants and other environmental stress affect the circulating haemocyte count both in quantity and quality [9,43]. In addition, the circulating haemocytes and non-circulating haemocytes exist in a state of dynamic flux [20].…”

“…One unit of enzyme specific activity was defined as the amount of enzyme being able to release 1 mM p-nitrophenol per minute per mg serum protein under the assay conditions. Acid phosphatase (EC 3.1.3.2) activity in serum was determined with spectrophotometric method [43]. A tube containing 0.2 ml serum, 0.5 ml 0.1 M sodium citrate buffer (pH 4.8) and 0.2 ml 10 mM p-nitrophenol phosphate (Sigma) was incubated in a 37 C water bath for 30 min.…”

Degradation of a Bitter Peptide Derived from Casein by Lactic Acid Bacterial Peptidase

“…Subcellular fractions Subcellular fractions were prepared according to Akuzawa & Fox (1998) and Coolbear et al (1992). From 100 mL of culture, 16.6 mL of loosely associated cell wall fraction (F1), 4.7 mL of cell wall lysate fraction (F2), 14 mL of cytoplasmic fraction (F3) and 2.7 mL of cell membrane fraction (F4) were obtained.…”

Separation of a milk acid phosphatase from a purified lactoferrin fraction and identification as a member of the mammalian purple acid phosphatase family

“…Enzyme activity was determined by a modification of the method used by Akuzawa and Fox (1998), with slight modifications as described by Miura et al (2010). Enzyme sample (100 mL) was added to 100 mL of 10 mM p-nitrophenylphosphate (p-NPP; Sigma Chemicals, St. Louis, MO, USA) in 10 mM sodium acetate (pH 5.2) and incubated for 30 min at 50 C. The reaction stopped by 200 mL of 1 M NaOH and absorbance was measured at 405 nm.…”