Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. I-450

Kumar, C. Ganesh; Joo, Han-Seung; Choi, Ji‐Won; Koo, Yoon-Moo; Chang, Robert Chen-Hao

doi:10.1016/j.enzmictec.2004.03.001

Cited by 304 publications

(145 citation statements)

References 21 publications

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“…3 a, b and c) of the three bioflocculants were analyzed according to Xiong et al (2010). It clearly showed the presence of carboxyl, hydroxyl, amide and amino groups in its molecules, which are the preferred groups for the flocculation, similar to those observed in polyelectrolytes (Ganesh- Kumar et al, 2004). The spectra showed a broad stretching intense peak displayed around 3440/cm that was characteristic of hydroxyl and amino groups (Liu et al, 2009).…”

Section: Characterization Of Bioflocculantsmentioning

confidence: 99%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:The aim of this study was to look for high efficient bioflocculant-producing microorganisms. Among 36 bacterial colonies isolated from a crude petroleum oil sample, three of them as Bacillus subtilis and Pseudomonas spp. exhibited flocculation activity exceeding 90 % after 3 days of cultivation. They were identified by 16 S rDNA sequence analysis as Bacillus subtilis and Pseudomonas sp. Spectroscopic analysis of the polymers by nuclear magnetic resonance and fourier-transform infrared revealed that the polymers were glycoproteins. These polymers were soluble in water and insoluble in any organic solvents tested. The effects of bioflocculant dosage, temperature and pH on the flocculation activity were evaluated. The maximum bioflocculation activities were observed at an optimum bioflocculant dosage of 3.5 mg/L (strains Bacillus subtilis and Pseudomonas) and 5.0 mg/L (strain CPO14), respectively. In addition, these biopolymers were able to flocculate kaolin suspension (5 g/L) over a wide range of pH (pH 3-9) and temperature (5-50 °C) tested in the presence of CaCl 2 . The highest flocculation activities of strains CPO8, CPO13 and CPO14 were 96.03 %, 92.17 % and 97.59 %, respectively in the early stationary phase (at 24 h), while the cell production reached its maximum in the stationary phase (at 72 h). Their efficient flocculation capabilities suggest potential applications in industries.

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Section: Characterization Of Bioflocculantsmentioning

confidence: 99%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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show abstract

“…The purified polysaccharides were ground with KBr powder and then pressed into pellets for FTIR measurement in the frequency range of 4000-500 cm À1 (Kumar, Joo, Choi, Koo, & Chang, 2004). …”

Section: Infrared Spectral Analysismentioning

confidence: 99%

Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

Luo

Zhou

et al. 2010

Carbohydrate Polymers

237

112

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“…The DNP was ground with KBr powder and then pressed into a polymer film for FTIR measurement in the frequency range of 4000-500 cm −1 [21].…”

Section: Infrared Spectral Analysismentioning

confidence: 99%

In vitro antioxidant activities of a water-soluble polysaccharide derived from Dendrobium nobile Lindl. extracts

Luo

Zhou

et al. 2009

International Journal of Biological Macromolecules

125

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Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. I-450

Cited by 304 publications

References 21 publications

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

In vitro antioxidant activities of a water-soluble polysaccharide derived from Dendrobium nobile Lindl. extracts

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