Purification and characterization of an extracellular xylanase produced by the endophytic fungus, Aspergillus terreus, grown in submerged fermentation

Sorgatto, M; Guimarães, Nca; Zanoelo, Fabiana Fonseca; Marques,; Sc, Peixoto-Nogueira; Giannesi, GG

doi:10.5897/ajb11.2686

Cited by 36 publications

(11 citation statements)

References 37 publications

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“…It is why in literature can be found a lot of works of xylanase production using wheat bran, for several Aspergillus species like, Aspergillus terreus (Sorgatto et al, [2012]); Aspergillus niger BCC14405 (Khonzue et al, [2011]); Aspergillus niger BC-1 (Dhillon et al, [2011]); Aspergillus terricola Marchal (Michelin et al, [2010]); Aspergillus foetidus MTCC 4898 (Chapla et al, [2010]); Aspergillus niger (Farinas et al, [2010]); Aspergillus niger DFR-5 (Pal and Khanum, [2010]); Aspergillus niveus RP05 (Peixoto-Nogueira et al, [2009]); Aspergillus niger , Aspergillus niveus and Aspergillus ochraceus (Betini et al, [2009]) and Aspergillus ficuum AF-98 (Fengxia et al, [2008]). Xylanase production on corncob and sugarcane bagasse was also reported for Aspergillus terreus (Sorgatto et al, [2012]), Chaetomium sp. CQ31 (Jiang et al, [2010]), Aspergillus foetidus MTCC 4898 (Chapla et al, [2010]), Aspergillus terreus (Lakshmi et al, [2009]), Thermomyces lanuginosus MC 134 (Kumar et al, [2009]) and Aspergillus niger ANL 301 (Okafor et al, [2007]).…”

Section: Resultsmentioning

confidence: 99%

“…The xylanase production has been reported for bacteria (Sepahy et al, [2011]), actinomycetes (Garg et al, [2011]) and fungi (Das et al, [2013]; Sorgatto et al, [2012]; Facchini et al, [2011, 2012]). Filamentous fungi which produce xylanases are attracting greater attention than bacteria and yeast because they are particularly interesting from an industrial point of view, due to the fact they secrete much higher xylanolytic enzymes into the medium (Okafor et al, [2007]; Polizeli et al, [2005]).…”

Section: Introductionmentioning

confidence: 99%

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Bioprocess and biotechnology: effect of xylanase from Aspergillus niger and Aspergillus flavus on pulp biobleaching and enzyme production using agroindustrial residues as substract

et al. 2013

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This study compares two xylanases produced by filamentous fungi such as A. niger and A. flavus using agroindustrial residues as substract and evaluated the effect of these enzymes on cellulose pulp biobleaching process. Wheat bran was the best carbon source for xylanase production by A. niger and A. flavus. The production of xylanase was 18 and 21% higher on wheat bran when we compare the xylanase production with xylan. At 50°C, the xylanase of A. niger retained over 85% activity with 2 h of incubation, and A. flavus had a half-life of more than 75 minutes. At 55°C, the xylanase produced by A. niger showed more stable than from A. flavus showing a half-life of more than 45 minutes. The xylanase activity of A. niger and A. flavus were somehow protected in the presence of glycerol 5% when compared to the control (without additives). On the biobleaching assay it was observed that the xylanase from A. flavus was more effective in comparison to A. niger. The kappa efficiency corresponded to 36.32 and 25.93, respectively. That is important to emphasize that the cellulase activity was either analyzed and significant levels were not detected, which explain why the viscosity was not significantly modified.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioprocess and biotechnology: effect of xylanase from Aspergillus niger and Aspergillus flavus on pulp biobleaching and enzyme production using agroindustrial residues as substract

et al. 2013

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“…Ability of A. niger to decompose various lignocellulosic substrates like wheat straw (Kaur and Sahota 2004), rice straw (Lee et al 2011) and litter (Song et al 2010) has been reported. Aspergillus terreus was reported as good producer of xylanase but having lower activity of cellulase in liquid medium having wheat bran as carbon source (Sorgatto et al 2012). Production of cellulases by A. terreus in fermentation of different substrates was reported by many authors like oil plam empty fruit bunch fiber (Shahriarinour et al 2011), rice straw and sugar cane bagasse (Kumar and Parikh 2015).…”

Section: Lignocellulolytic Activity In Solid State Fermentationmentioning

confidence: 99%

Crop residue degradation by fungi isolated from conservation agriculture fields under rice–wheat system of North-West India

Choudhary

Sharma

Jat

et al. 2016

Int J Recycl Org Waste Agricult

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Purpose In North West-Indo Gangetic Plains (NW-IGP) of India in situ burning of crop residues is practiced by majority of farmers' which deteriorates soil and environmental quality. Fungi have the potential for lignocellulose degradation and can be used for the in situ decomposition of crop residues. Lignocellulose degrading fungal spp. were isolated and evaluated for the activity of lignocellulolytic enzymes. Method The lignocellulose degrading fungi were isolated by appearance of zone on carboxy methyl cellulose (CMC) agar media and tannic acid (TA) media. Carboxy methyl cellulase, filter paperase, cellobiase, xylanase and laccase activity were estimated in submerged, as well as solid state fermentation using a mixture of rice and wheat straw in the ratio of 4:1. rice-wheat straw as substrate. The residue left after solid state fermentation was evaluated for carbon/nitrogen ratio, dry mass loss, and loss of cellulose, hemicellulose and lignin. Selected potential isolates were further tested in pot experiment for their effect on wheat plants. The interaction among isolates was also studied. Result After primary screening, 19 out of a total of 72 fungal isolates were selected based on their enzymatic activity profile and potential to degrade lignocellulosic residues in submerged fermentation. Out of these 19 isolates, 11 were further selected based on their enzymatic secretions in solid state fermentation. All the 11 strains were identified morphologically. Four fungal isolates (RPW 1/3, RPW 1/6, RPWM 2/2 and RZWM 3/2) showed higher enzymatic activities and more loss of dry mass and cell wall constituents over the other isolates. These isolates were identified by ITS region sequencing as Aspergillus flavus, Aspergillus terreus, Penicillium pinophilum and Alternaria alternata. Conclusion This study revealed that fungal isolates may be used for managing crop residues in conservation agriculture based rice-wheat system of NW-IGP to eliminate ill effects of residue burning.

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“…In studies with A. terreus, the xylanase was thermotolerant at 45 and 50°C, but had half-life of only 25 min at 50°C (Sorgatto et al, 2012). And the xylanase of A. phoenicis had a half-life of only 25 min at 50°C (Rizzatti et al, 2001).…”

Section: Effect Of Different Carbon Sources On Xylanase Production Bymentioning

confidence: 99%

Production of cellulase-free xylanase by Aspergillus flavus: Effect of polyols on the thermostability and its application on cellulose pulp biobleaching

Silva¹,

Guimarães²,

Peixoto-Nogueira³

et al. 2015

Afr. J. Biotechnol.

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The production of xylanase without cellulase is required for prebleaching of pulp in pulp and paper industry. Aspergillus flavus produced high levels of xylanase on agricultural residues with wheat bran and sugarcane bagasse (4.17 U/mg), and wheat bran and corncob (2.97 U/mg). Xylanase was found to be stable at 45°C with 100% of its original activity remaining after 2 h incubation. At 50°C, xylanase was stable for the first twenty minutes, and had half-life of 50 min. The pH stability for the xylanase from A. flavus was most stable in the range of pH 3.0-8.0 retaining more that 100% activity after 1 h. The addition of 5% glycerol, mannitol or xylitol protected the xylanase from thermal inactivation at 50°C. The protective effect by glycerol, xylitol and mannitol resulted in increases of 162, 262.5 and 150% when compared with the control at 120 min, approximately. Increasing the polyols concentration up to 20% (w/v) further improved the thermostability of xylanase after 120 min at 50°C by 300% when compared with the control (no additive). The kappa number reduced 2.56 points, which corresponds to 18.34 kappa efficiency. This xylanase is an attractive enzyme for potential future application in the pulp and paper industries, since industrial application requires a cellulase-free activity, maintenance of high temperature and enzyme stability are desirable.

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Purification and characterization of an extracellular xylanase produced by the endophytic fungus, Aspergillus terreus, grown in submerged fermentation

Cited by 36 publications

References 37 publications

Bioprocess and biotechnology: effect of xylanase from Aspergillus niger and Aspergillus flavus on pulp biobleaching and enzyme production using agroindustrial residues as substract

Bioprocess and biotechnology: effect of xylanase from Aspergillus niger and Aspergillus flavus on pulp biobleaching and enzyme production using agroindustrial residues as substract

Crop residue degradation by fungi isolated from conservation agriculture fields under rice–wheat system of North-West India

Production of cellulase-free xylanase by Aspergillus flavus: Effect of polyols on the thermostability and its application on cellulose pulp biobleaching

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