Purification and Characterization of a Lectin from Crotalaria paulina Seeds

Pando, Luzia Aparecida; Carvalho, D; Toyama, Marcos H.; Ciero, Luciana Di; Novello, José Camillo; Pascholatti, Sérgio F.; Marangoni, Sérgio

doi:10.1007/s10930-004-5219-9

Cited by 11 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The subunit molecular mass is in the vicinity of 30 kDa, similar to the molecular masses of some chitinases [27][28][29][30][31][32][33][34][35]. and the subunit molecular masses of some agglutinins/lectins [36][37][38][39].…”

Section: Discussionsupporting

confidence: 55%

Isolation of a Mitogenic Agglutinin with Relatively High Thermostability from Seeds of the Variegated Shell Ginger

Wong

Ng²,

Zhang³

et al. 2010

PPL

View full text Add to dashboard Cite

An agglutinin with a molecular mass of 130 kDa has been isolated from the seeds of Alpinia zerumbet cv.'Variegata'. The isolation procedure involved anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. The agglutinin exhibited hemagglutinating activity toward rabbit erythrocytes which could not be inhibited by simple sugars. It was composed of four identical 32-kDa subunits with substantial N-terminal sequence similarity to chitinase and yieldin. The hemagglutinating activity of A. zerumbet agglutinin was stable up to 80(o)C and not affected by presence of a variety of salts. The agglutinin stimulated [methyl-(3)H]-thymidine uptake by mouse splenocytes. It did not exhibit antifungal activity.

show abstract

Section: Discussionsupporting

confidence: 55%

Isolation of a Mitogenic Agglutinin with Relatively High Thermostability from Seeds of the Variegated Shell Ginger

Wong

Ng²,

Zhang³

et al. 2010

PPL

View full text Add to dashboard Cite

show abstract

“…Finally, trichomes are also unlikely to be the main driver of local adaptation patterns because, in the laboratory tests, leaves were offered only during the first two days, and larval mortality during this period was similar in the two plant populations [18]. Therefore, the patterns of adaptation observed at the continental scale may be driven by another resistance trait not yet measured (possible candidate traits include isoflavonoids, non-protein amino acids and proteinase inhibitors [28]–[32]), or, more likely, by the emerging property of the synergistic effect of several of these traits [27].…”

Section: Discussionmentioning

confidence: 99%

“…These represent a small subset of all the possible traits that may affect U. ornatrix . For example, there is sparse information that Crotalaria plants may also have other chemical defenses such as isoflavonoids, non-protein amino acids and proteinase inhibitors [28]–[32], classes of compounds that we were not equipped to measure.…”

Section: Methodsmentioning

confidence: 99%

Varying Herbivore Population Structure Correlates with Lack of Local Adaptation in a Geographic Variable Plant-Herbivore Interaction

2011

View full text Add to dashboard Cite

Local adaptation of parasites to their hosts due to coevolution is a central prediction of many theories in evolutionary biology. However, empirical studies looking for parasite local adaptation show great variation in outcomes, and the reasons for such variation are largely unknown. In a previous study, we showed adaptive differentiation in the arctiid moth Utetheisa ornatrix to its host plant, the pyrrolizidine alkaloid-bearing legume Crotalaria pallida, at the continental scale, but found no differentiation at the regional scale. In the present study, we sampled the same sites to investigate factors that may contribute to the lack of differentiation at the regional scale. We performed field observations that show that specialist and non-specialist polyphagous herbivore incidence varies among populations at both scales. With a series of common-garden experiments we show that some plant traits that may affect herbivory (pyrrolizidine alkaloids and extrafloral nectaries) vary at the regional scale, while other traits (trichomes and nitrogen content) just vary at the continental scale. These results, combined with our previous evidence for plant population differentiation based on larval performance on fresh fruits, suggest that U. ornatrix is subjected to divergent selection even at the regional scale. Finally, with a microsatellite study we investigated population structure of U. ornatrix. We found that population structure is not stable over time: we found population differentiation at the regional scale in the first year of sampling, but not in the second year. Unstable population structure of the herbivore is the most likely cause of the lack of regional adaptation.

show abstract

“…2,7 , C. juncea L. 8 , C. agatiflora S. 9 , C. paulina S. 10 , C. medicaginea L. 11 , C. striata D. 12 e C. mucronata D.…”

Section: Resultsunclassified

Purificação e Atividade Hemaglutinante das Lectinas de Sementes de Crotalaria spectabilis R.

Oliveira

Rego²,

Ristow³

et al. 2013

BBR - Biochem. Biotechnol.

View full text Add to dashboard Cite

RESUMOLectinas são proteínas que se ligam com afinidade a resíduos de glicídios com capacidade de aglutinar células e preciptar glicoconjugados. Essas proteínas são importantes porque estão envolvidas no processo de reconhecimento celular e sinalização em diversas vias metabólicas. O objetivo deste estudo foi isolar, purificar e investigar a atividade biológica das lectinas de Crotalaria spectabilis R. A extração foi feita das sementes, as quais foram moidas e suas células lisadas em solução NaCl 0,15 M. Seguiu-se precipitação protéica por acetona e sulfato de amônio. A partir dos dados da quantificação, observou-se que o método de precipitação salina apresentou maior rendimento de proteínas. Os ensaios de hemaglutinação foram positivos para atividade hemaglutinante. Assim, a partir dos dados obtidos concluímos que nas sementes de C. spectabilis R. existem lectinas com capacidade de reconhecer os receptores presentes na membrana dos eritrócitos humanos ligando-se a estes e promovendo a aglutinação celular. Palavras-chave:Especificidade, Hemaglutinação, Receptores. INTRODUÇÃOLectinas são proteínas com capacidade de se ligarem especificamente a carboidratos e aglutinarem células. Nas plantas estão presentes em todos os órgãos, embora encontradas em maior quantidade nas sementes de leguminosas. Apresentam distribuição e atividade em todos os organismos vivos ocorrendo desde vírus até mamíferos 1,2 , embora tenham sido pesquisadas em maior número em plantas 3,4 . O objetivo da pesquisa foi isolar, purificar e investigar a atividade biológica das lectinas extraídas de sementes de Crotalaria spectabilis R., para que possam ser utilizadas como uma ferramenta molecular em áreas como oncologia, bacteriologia, virologia entre outras aplicações. Dessa forma, pesquisas sobre a atividade biológica de lectinas das sementes de Crotalaria spectabilis R. são importantes para a compreensão de processos envolvidos no reconhecimento celular, para que funcionem como uma ferramenta molecular em novos estudos, assim como na descoberta de produtos biotecnológicos inovadores.

show abstract

Purification and Characterization of a Lectin from Crotalaria paulina Seeds

Cited by 11 publications

References 0 publications

Isolation of a Mitogenic Agglutinin with Relatively High Thermostability from Seeds of the Variegated Shell Ginger

Isolation of a Mitogenic Agglutinin with Relatively High Thermostability from Seeds of the Variegated Shell Ginger

Varying Herbivore Population Structure Correlates with Lack of Local Adaptation in a Geographic Variable Plant-Herbivore Interaction

Purificação e Atividade Hemaglutinante das Lectinas de Sementes de Crotalaria spectabilis R.

Contact Info

Product

Resources

About