Purification and characterization of a xylanase from Bipolaris sorokiniana

Peltonen, Sari; Karjalainen, Reijo; Niku‐Paavola, Marja‐Leena

doi:10.1016/s0953-7562(09)80339-0

Cited by 12 publications

(2 citation statements)

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“…The xylanase preparation used in this study was purified from the B. sorokiniana culture filtrates grown on barley cell walls (Peltonen et al,, 1994). The enzyme was diluted (20 /ig mr', 5 //g ml"', 50 ng ml"') in 10 mM phosphate buffer (pH 6.0), In wheat leaves, only the xylanase at 20 /Ig ml^' was used.…”

Section: Materials and Methods Plant Materialsmentioning

confidence: 99%

“…Helminthosporium sativum Pammel, King and Bakke) is a widespread necrotrophic fungus; barley and wheat are the most important cereal hosts (Tinline, 1988). It causes common root rot, seedhng blight, black nodes and stem breakage, spot blotch of leaves, headblight and seed smudge (Jergensen, 1974;Raemakers and Tinline, 1981;Mathre, 1982), Peltonen et al (1994) showed that xylanase activity in B. sorokiniana was high compared with other cell wall degrading enzymes when the fungus was grown in media containing barley cell wall preparation as the sole carbon source. It has been suggested that xylanases are involved in many host-pathogen systems (Hancock, 1967;Van Etten and Bateman, 1969;Mullen and Bateman, 1975;Cooper et al, 1988), but their role in pathogenesis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Phenylalanine Ammonia‐lyase Activity in Barley After Infection with Bipolaris sorokiniana or Treatment with its Purified Xylanase

Peltonen

Karjalainen

1995

Journal of Phytopathology

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Phenylalanine ammonia‐lyase (PAL) activity was determined from leaves and roots of two barley (Hordeum vulgare L.) cultivars after infection with a necrotrophic pathogen, Bipolaris sorokiniana (Sacc.) Shoem., and treatment with its purified xylanase. PAL activity increased in leaves of both cultivars 16 h after fungal inoculation but two phases, with activity peaks at 24–32 h and 40 h, were recorded only for the more resistant cultivar, Agneta. Attempts to use a PAL inhibitor, χ‐amin, ooxyacetic acid, to increase susceptibility to B. sorokiniana in barley leaves were unsuccessful. Treatments of leaves with purified xylanase resulted in more rapid (4–12 h after injection), although reduced, induction of PAL compared with fungal injection. The higher the concentration of xylanase applied the earlier the activity peaks were detected. Fungal inoculation only slightly increased PAL activity in barley roots while xylanase treatment had no effect. The basal level of PAL was however much higher in roots than in leaves. In wheat, Triticum aestivum L. resistant to B. sorokiniana, the time‐course of PAL induction after fungal infection and xylanase treatment resembled that for cv. Agneta, while in oats, Avena sativa L. (non‐host) PAL activity did not change after the treatments. The results suggest that the second phase of PAL induction, associated only with responses of barley cv. Agneta and wheat, is linked with their resistance to B. sorokiniana infection. The possible role of xylanase as an elicitor of PAL is discussed.

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Section: Materials and Methods Plant Materialsmentioning

confidence: 99%